Product Information
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
SUPPLIED REAGENTS:
ADDITIONAL REAGENTS (NOT SUPPLIED):
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
NOTE: This protocol begins after membrane exposure to film or digital imager. Best results are obtained if the membrane is not allowed to dry. Membranes can be stored in 1X TBST at 4℃.
NOTE: Quantitative comparison of targets before and after stripping is not recommended due to the removal of small amounts of membrane-bound protein with each round of stripping.
Protocol Id: 2564
Having to re-run gels and repeat western blotting efforts is costly and time-consuming. Western Blot Stripping Buffer (5X) enables the membranes to be reused with the added benefit of not having to re-load expensive or limited samples into the gel. Allowing for the detection of a different target with an alternative primary antibody or by reprobing with a different concentration of a primary antibody saves valuable time.
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Cat. # | Size | Qty. | Price |
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91925S | 100 ml (25 western blots) |
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