AcetylScan® (Acetylation Proteomics)
Histone acetyltransferases (HATs) and histone deacetylases (HDACs) have regulator functions in numerous aspects of cell physiology. AcetylScan® is a unique and unparalleled strategy for global analysis of HDAC and HAT activity on protein acetylation. Our AcetylScan Services utilize antibodies with high affinity to acetylated-lysine (Ac-K) peptides for enrichment of acetylated peptides from protease digested cell samples, followed by LC-MS/MS analysis for quantitative profiles of many hundreds of non-redundant acetylated sequences.
AcetylScan® Services have been applied to class I, II and III HDAC studies, and have been performed on sub-cellular compartment fractions for substrate, target pathway and compound biomarker discovery.
AcetylScan® Workflow Diagram 0.5 MB
Example Studies and Deliverables
|Description||Antibody||Kit||Consenus Site||LCMS Data & Output Results|
|Acetylated-Lysine||9814||Ac-K||Mouse Liver XLS RAW|