Frequently Asked Questions
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Why did my antibody ship at room temperature?
In line with Cell Signaling Technology’s (CST) environmentally conscientious efforts, we having been shipping antibodies at room temperature since 2003 when possible to reduce packing materials waste. Each CST™ antibody is stability tested by exposure to room temperature and 37°C for one week followed by western blot testing to ensure no change in antibody performance. If you received an antibody at room temperature, rest assured that it has passed stability testing and that the antibody performance is still optimal. Upon receipt, please store the antibody as indicated on the product datasheet.
How should I store my antibody tube? How much does temperature affect stability?
Please store the antibodies in the original tube at -20°C and do not aliquot as this may introduce variables and waste antibody. The antibodies are provided in a buffer that contains 50% glycerol and do not freeze under the recommended storage conditions. This prevents degradation of the antibody due to repeated freeze-thaw cycles. Conjugated antibodies may indicate storage at 4°C, please store as suggested on the product datasheet.
Have species been tested if they are not listed as cross-reactive with a CST™ antibody on the datasheet?
Possibly. CST scientists test all CST antibodies on multiple species, if possible (this depends on the target and the experimental systems available). If tested species do not cross-react, we do not list that species as cross-reactive. However, it is possible that the model we test may contain a low level of particular protein or the inductions we used are not ideal in that particular model system. If you need additional information regarding cross-reactivity with a particular species, please feel free to contact CST technical support team at firstname.lastname@example.org for assistance.
Why can't I use my own western blotting protocol with CST antibodies?
CST™ antibodies are optimized to provide the strongest signal with the least background, using our standard western blotting protocol. Using the recommended, optimized CST protocol saves you costly time and materials.
Does this antibody work for my application (western blot, immunoprecipitation, immunofluorescence, immunohistochemistry, flow cytometry, chromatin immunoprecipitation)?
CST routinely tests antibodies in relevant applications. Those that pass rigorous application-specific validation standards are recommended. We only recommend applications that have passed rigorous validation standards, if performance data is ambiguous we do not recommend the application. Please contact the CST technical support team at email@example.com for more information about a particular antibody, or for a recommendation on which antibody will perform best in your desired application.
Does this antibody work for fluorescent western blotting?
CST does not routinely test antibodies for fluoresecent western blotting, though many work well in this application. Performing a fluorescent western blot using CST™ antibodies requires a modified immunoblotting protocol. We find that omitting Tween® 20 detergent from the blocking step (using only 5% nonfat dry milk in TBS) and drying the membrane prior to imaging are the only necessary changes from our standard protocol. Full details can be found in our Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In BSA) and our Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In Milk). Additionally, our Prestained Protein Marker, Broad Range (Premixed Format) #7720 serves well as a marker in fluorescent western blotting because it is autofluorescent at near-infrared wavelengths.
When there are multiple antibody choices, which will work optimally for in my application?
Often, CST offers more than one antibody against a target. To chose the antibody that will perform the best in your desired application, please visit our Antibody Comparison Tables or contact CST technical support at firstname.lastname@example.org for assistance in selecting the optimal antibody for your application.
How does CST ensure the quality of their antibodies?
CST scientists test all our products in relevant applications such as western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, flow cytometry, and chromatin immunoprecipitation. When an antibody is recommend for a particular application, it indicates that the antibody has passed rigorous application-specific testing standards. Additionally, our scientists create specialized (optimized) immunostaining protocols for individual products, saving you time and reagents. We routinely test our antibodies on multiple species including human, monkey, mouse, and rat. The high quality of our products is the most important consideration at CST. If you are interested in a particular application not listed on the product webpage, please contact CST technical support at email@example.com for further information.
How does CST quality control for different lots?
CST scientists strive to achieve product consistency from lot to lot. As a standard, we routinely test the new lot in all the applications we recommend side-by-side with the old lot. The recommended dilution for each application is optimized for each lot following our protocols. Please visit our western blot validation webpage to learn more.
What is the concentration of my antibody?
At CST, we formulate antibodies based on activity to eliminate the necessity for you to make adjustments. Concentrations between lots of antibodies are sometimes adjusted to optimize results. For the concentration of a specific antibody lot, please contact CST technical support at firstname.lastname@example.org.
Can I order a custom formulation of this antibody?
For custom (carrier-free) orders, many antibodies prepared formulated in PBS without BSA or glycerol are in stock. This formulation is amenable to conjugation and can be conjugated to fluorophores, beads, or dyes. In order to meet the requirements of your assay or your laboratory, we are also happy to provide you with bulk sales. Please contact us for pricing and availability at email@example.com.
Could CST develop an antibody for me?
CST does not develop custom antibodies, but we are happy to accept your target suggestions. Please send your suggestion to firstname.lastname@example.org and a CST scientist will contact you shortly.
Need further help?
For general product questions, contact email@example.com.
updated February 2013