Protocols

For the best possible results, Cell Signaling Technology (CST) strongly recommends using our optimized application-specific protocols for each product. These protocols are the result of extensive in-house validation performed at CST and ensure accurate and reproducible results.

Product specific protocols will be linked from matching product web pages.

Western Blotting (Immunoblot)

• Western Immunoblotting Protocol (Western)
• Western Immunoblotting (Fluorescent) Protocol (Fluorescent Western)

Immunoprecipitation

• Immunoprecipitation Protocol / (For Native Protein) (IP / Native Protein)
• Immunoprecipitation Protocol Utilizing Magnetic Separation / (For Analysis By Western Immunoblotting) (IP Magnetic)
• Immobilized Immunoprecipitation Protocol / (For Analysis By Western Immunoblotting) (Immobilized IP)

Immunohistochemistry

• Immunohistochemistry Protocol (Paraffin) for SignalStain^® Boost Detection Reagent (IHC / Paraffin)
• Immunohistochemistry Protocol (Frozen) for SignalStain^® Boost Detection Reagent (IHC / Frozen)
• Fluorescent Multiplex Immunohistochemistry Protocol (mIHC/Paraffin)
• Immunohistochemistry Leica^® BOND™ Protocol

Immunofluorescence

• Immunofluorescence Protocol with Formaldehyde Fixation
• Immunofluorescence Protocol for Cell-based Assays Requiring Methanol Fixation
• Immunofluorescence Protocol with Methanol Permeabilization (IF Methanol-perm)

Flow Cytometry

• Flow Cytometry Methanol Permeabilization Protocol
• Flow Cytometry Triton™ X-100 Permeabilization Protocol
• Flow Cytometry FoxP3/Transcription Factor Fixation and Permeabilization Protocol
• Flow Cytometry Live Cell Protocol

Chromatin Immunoprecipitation

• SimpleChIP^® Chromatin Immunoprecipitation Protocol (Agarose Beads) (ChIP Agarose)
• SimpleChIP^® Chromatin Immunoprecipitation Protocol (Magnetic Beads) (ChIP Magnetic)
• SimpleChIP^® Plus Chromatin Immunoprecipitation Protocol (Agarose Beads) (ChIP Agarose)
• SimpleChIP^® Plus Chromatin Immunoprecipitation Protocol (Magnetic Beads) (ChIP Magnetic)
• SimpleChIP^® Plus Sonication Chromatin Immunoprecipitation Protocol (Magnetic Beads) (ChIP Magnetic)
• DNA Library Prep Kit for Illumina^® Protocol (ChIP-seq, CUT&RUN)
• Multiplex Oligos for Illumina^® Protocol (Single Index Primers) (ChIP-seq, CUT&RUN)
• Multiplex Oligos for Illumina^® Protocol (Dual Index Primers) (ChIP-seq, CUT&RUN)

CUT&RUN

• CUT&RUN Protocol
• Digestion of CUT&RUN Input DNA by MNase
• DNA Library Prep Kit for Illumina^® Protocol (ChIP-seq, CUT&RUN)
• Multiplex Oligos for Illumina^® Protocol (Single Index Primers) (ChIP-seq, CUT&RUN)
• Multiplex Oligos for Illumina^® Protocol (Dual Index Primers) (ChIP-seq, CUT&RUN)

CUT&Tag

• CUT&Tag Protocol
• CUT&Tag Dual Index Primers and PCR Master Mix

Sandwich Immunoassays (ELISA)

• FastScan^® Sandwich ELISA Protocol (Colormetric ELISA)
• PathScan^® Sandwich ELISA Protocol (Colormetric ELISA)
• PathScan^® Sandwich ELISA Protocol (Colormetric ELISA - for kits with Lyophilized Antibodies)
• PathScan^® Sandwich ELISA Protocol (Chemiluminescent ELISA)
• PathScan^® Sandwich ELISA Protocol (Chemiluminescent ELISA - for kits with Lyophilized Antibodies)
• PathScan^® Sandwich ELISA Antibody Pair Protocol (ELISA-Pair)
• ELISA-Peptide Assay Protocol (ELISA-P)
• HRP Chemiluminescence ELISA-P (HRP Chemiluminescent ELISA-P)

In-Cell Western

• In-Cell Western Protocol (In-Cell Western)

SignalStar Multiplex IHC

• SignalStar™ Multiplex Immunohistochemistry Assay Manual
• SignalStar™ Multiplex Immunohistochemistry Assay for Use on BOND RX Fully Automated Research Stainer by Leica Biosystems

Resources

• Troubleshooting
• Technical Documentation
• Technical Support