SignalSilence® siRNA Reagents
SignalSilence® siRNA reagents from Cell Signaling Technology (CST) allow the researcher to specifically inhibit protein expression. These products utilize RNA interference, a method in which gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. Two equally potent siRNAs are available for each target (siRNA I and II). In addition, a fluorescein-labeled non-targeted siRNA control #6201 allows the user to monitor transfection efficiency, while an unconjugated control siRNA #6568 can be used to control for specificity. SignalSilence® siRNA for both human and mouse targets are now available.
SignalSilence® LC3B siRNA
Confocal immunofluorescent analysis of chloroquine-treated HeLa cells, transfected with SignalSilence® Control siRNA (Unconjugated) #6568 (left) or transfected with SignalSilence® LC3B siRNA I #6212 and SignalSilence® LC3B siRNA II #6213 (right), using LC3B (D11) XP® Rabbit mAb #3868 (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
SignalSilence® c-Myc siRNA
Confocal immunofluorescent analysis of HeLa cells, transfected with SignalSilence® Control siRNA (Unconjugated) #6568 (left) or transfected with SignalSilence® c-Myc siRNA I #6341 (right), using c-Myc (D84C12) XP® Rabbit mAb #5605 (green). Actin filaments have been labeled wth DY-554 phalloidin (red).