CUT&RUN Resource Center

CUT&RUN Protocol: A Visual Guide

CUT&RUN: An Introduction

Cleavage Under Targets & Release Using Nuclease (CUT&RUN) is a low-cell number chromatin profiling technology that utilizes target-specific primary antibodies and pAG-MNase to isolate protein-DNA complexes on native chromatin for analysis by qPCR or next-generation sequencing (NGS). The assay can also be implemented with two types of spike-in normalization controls to correct for technical variability between samples. Drosophila spike-in provides end-to-end normalization from cells through sequenced DNA, while fragmented yeast DNA provides downstream normalization from DNA purification through sequenced DNA. We have compiled a collection of videos, application notes, eBooks, blogs, and more to demonstrate how CUT&RUN is compatible with histones, transcription factors, and cofactors, allows you to progress from cell to DNA in 1–2 days, and requires fewer NGS reads per sample due to very low background signal.

_{U.S. Patent No. 11,733,248, foreign equivalents, and child patents deriving therefrom.}

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_{U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.}

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