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Matched Antibody Pairs and ELISA-Validated Antibodies

Antibodies Shouldn’t Be a Variable in Your Immunoassay Development

The success of your immunoassay development depends on identifying the optimal antibody pair to ensure reproducible and reliable results. Finding the ideal pair is time-consuming and if it doesn’t meet specificity and sensitivity requirements, it can result in the loss of more time and resources.

Cell Signaling Technology scientists perform sandwich ELISA to identify and validate capture and detection antibodies, and all matched antibody pairs and ELISA-validated antibodies are provided carrier-free (BSA-and azide-free at ≥ 1 mg/ml in 100 µl) making them conjugation-ready for assay platform-compatibility:

  • Meso Scale Discovery (MSD)
  • Alpha Technologies (AlphaLISA, AlphaScreen)
  • TR-FRET, HTRF, LANCE
  • Luminex xMAP
  • Quanterix Simoa

Streamline your assay development and project timelines with assay results that are accurate and reproducible at scale.

Find the Validated Antibodies for Your Assay

Browse a selection of ready-to-ship matched antibody pairs, ELISA-validated antibodies, and matched antibody pairs for custom order.

Matched Antibody Pairs

  • 285+ ready-to-ship pairs and growing
  • Detect key signaling nodes/readouts and biomarkers across multiple diseases
  • Pairs share non-overlapping epitopes and deliver the required specificity and sensitivity for your assay
  • Carrier-free and conjugation-ready

ELISA-Validated Antibodies

CST Solutions for Drug Discovery and Immunoassay Development

Drug Discovery Solutions Powered by CST

De-risk your drug discovery and development projects through a comprehensive portfolio of products and services including:

  • Tools and resources for research in oncology, immunotherapy, neurodegeneration, and targeted protein degradation.
  • Products and services like custom, biosimilar antibodies, proteomics analytical services, carrier-free custom formulations, and more.
  • Platform-ready antibodies for high-throughput assays including high-content analysis, automated western blotting, and multiplex IHC.

Solutions for Antibody Pair-Based Immunoassay Development

Learn how CST can support your assay development projects throughout their lifetime:

  • Solutions and products to kick off assay development projects efficiently.
  • Services to customize and tailor solutions specific to your assay requirements.
  • Bulk ordering and lot reservations to future-proof assay projects.

Custom Order Matched Antibody Pairs

Additional antibody pairs and validated antibodies are available for custom order. Once you have identified your selection, click the button below to submit your custom order.

Find Your Matched Antibody Pair

Custom Matched Antibody Pairs
Protein Target Site Specificity Species Reactivity

Phospho-c-Abl

Tyr412

H

ACE2

Total

H

Axl

Total

H

B7-H4

Total

H

BAX

Total

H

BCKDH-E1α

Total

H, M, R

Phospho-BCKDH-E1α

Ser293

H, M, R

Bcl-2

Total

H

Phospho-Beclin-1

Ser30

H

Bmi1

Total

H, M, Mk

BRD4 Isoform C

Total

H

CD79B

Total

H

CDK5

Total

H, M, R, Mk

Phospho-Cofilin

Ser3

H, M

DPP4/CD26

Total

H

Human EGF

Total

H

FAK

Total

H, M

Phospho-FAK

Tyr397

H

Phospho-FRA1

Ser265

H

Gasdermin D

Total

H

Cleaved Gasdermin D

Asp276

M

GCN2

Total

H

GDF15/MIC1

Total

H

Glucocorticoid Receptor

Total

H, M

Phospho-Glucocorticoid Receptor

Ser134

H, R

Ser226

H, M, R

GSK-3α

Total

H, M, R, Mk

Phospho-GSK-3α

Ser21

H, M, R, Mk

HDAC4

Total

H, M, Mk

HIF-1α

Total

H, M, R, Mk

Human IL-4

Total

H

Human IL-8

Total

H

Human IL-17A

Total

H

Phospho-Insulin Receptor β

Tyr1345

H, M

IRF-7

Total

M

Phospho-IRF-7

Ser437/438

M

KEAP1

Total

H

LbCpf1 (Strain ND2006)

Total

All

LC3A

Total

H, M

Human LIF

Total

H

Lysozyme C

Total

H

MEK1

Total

H, M

Phospho-MEK1

Ser217/221

H, M

p27 Kip1

Total

H, M

p38α MAPK

Total

H, M, R, Mk

Phospho-p70 S6 Kinase

Thr389

H, M, R, Mk

PTEN

Total

H, M, R, Mk

Phospho-PTEN

Ser380

H, M, Mk

B-Raf

Total

H, M, R, Mk

c-RAF

Total

H, M, R, Mk

S100A9

Total

H

SARS-CoV-2 Nucleocapsid Protein

Total

Vir

SynGAP

Total

H, M, R

TFEB

Total

H

Phospho-TFEB

Ser211

H

Human TNF-α

Total

H

TrkA

Total

H

Phospho-TrkA

Tyr674/Tyr675

H

ULK1

ULK1

H, M

Phospho-ULK1

Ser555

H, M

Ser638

H, M

Ser757

H, M

Species Reactivity Key: H—Human M—Mouse R—Rat Mk—Monkey B—Bovine Pg—Pig Mi—Mink Dm—D. melanogaster Z—Zebrafish Sc—S. cerevisiae

Matched Antibody Pair FAQs

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