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PTMScan® Motif Antibody Kits

The PTMScan® Motif Antibody Kits are a product line that enables the use of PTMScan Technology, a proprietary technology developed at Cell Signaling Technology (CST). The kits provide a selection of our proprietary Motif Antibodies ready for use in studies that employ PTMScan Technology. These antibodies are identical to those utilized by scientists at Cell Signaling Technology in PTMScan Discovery Services. For our customers with in-house expertise in mass spectrometry proteomics, these kits provide a cost-effective means of accessing any of the CST Motif IAP Beads and a Limited Use License to perform PTMScan Technology. Reagents for 10 assays as well as detailed protocols are provided in each kit.

Why consider antibody enrichment for studying phosphorylation?

  • Focused discovery of phosphosites based on amino acid (S/T/Y) or motif of interest
  • Increased coverage of lower occurring phosphorylation events compared to IMAC
  • View data showing complementarity between antibody enrichment and IMAC

PTMScan® Motif Antibody Kits

No.PTMScan® Kit DescriptionRecommended Protease TreatmentModification TypeLCMS Data & Output Results
No.PTMScan® Kit DescriptionRecommended Protease TreatmentModification TypeLCMS Data & Output Results
59322PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) KitTrypsin

WALP
Ubiquitin

SUMOylation
Mouse Liver
XLS  |  RAW
HEK293 Cells
XLS  |  RAW
13416PTMScan® Acetyl-Lysine Motif [Ac-K] KitTrypsinAcetylationMouse Liver
XLS  |  RAW 1 2
38572PTMScan® HS Phospho-Tyrosine (P-Tyr-1000) Kit #38572TrypsinPhosphorylationHCT-116 Cells
XLS  |  RAW 1 2
14499PTMScan® Pilot Acetyl-Lysine Motif [Ac-K] KitTrypsinAcetylationMouse Liver
XLS  |  RAW 1 2
5561PTMScan® Phospho-Akt Substrate Motif mAb 1 (RXXS*/T*) KitLysC*PhosphorylationMouse Liver
XLS  |  RAW 1 2
5563PTMScan® Phospho-Akt Substrate Motif mAb 2 (RXRXXS*/T*) KitLysC*PhosphorylationMouse Liver
XLS  |  RAW 1 2
5564PTMScan® Phospho-AMPK Substrate Motif (LXRXXS*/T*) KitLysC*PhosphorylationMouse Liver
XLS  |  RAW 1 2
12267PTMScan® Phospho-ATM/ATR Substrate Motif [pSQ] KitTrypsinPhosphorylationMouse Liver
XLS  |  RAW 1 2
12235PTMScan® Mono-Methyl Arginine Motif [mme-RG] KitTrypsin*MethylationMouse Liver
XLS  |  RAW 1 2
13474PTMScan® Asymmetric Di-Methyl Arginine Motif [adme-R] KitTrypsin*MethylationMouse Liver
XLS  |  RAW 1 2
13563PTMScan® Symmetric Di-Methyl Arginine Motif [sdme-RG] KitTrypsin*MethylationMouse Liver
XLS  |  RAW 1 2
12810PTMScan® Cleaved Caspase Substrate Motif [DE(T/S/A)D] KitTrypsinCleavedMouse Liver
XLS  |  RAW 1 2
12170PTMScan® Phospho-CK2 Substrate Motif (S*/T*DXE) KitTrypsinPhosphorylationMouse Liver
XLS  |  RAW 1 2
14809PTMScan® Pan-Methyl Lysine KitTrypsin*MethylationMouse Liver
XLS  |  RAW 1 2
14990PTMScan® Phospho-MAPK Substrate Motif [PXpTP] KitTrypsinPhosphorylationMouse Liver
XLS  |  RAW 1 2
4652PTMScan® Phospho-MAPK/CDK Substrate Motif (PXS*P and S*PXK/R) KitTrypsinPhosphorylationMouse Liver
XLS  |  RAW 1 2
5565PTMScan® Phospho-PKA Substrate Motif (RRXS*/T*) KitLysC*PhosphorylationMouse Liver
XLS  |  RAW 1 2
14989PTMScan® Phospho-Ser-Pro-Pro Motif [pSPP] KitTrypsinPhosphorylationMouse Liver
XLS  |  RAW 1 2
5636PTMScan® Phospho-Tyrosine Mouse mAb (P-Tyr-100) KitTrypsinPhosphorylationMouse Brain
XLS  |  RAW
8803PTMScan® Phospho-Tyrosine Rabbit mAb (P-Tyr-1000) KitTrypsinPhosphorylationMouse Liver | XLS | RAW 1 2
14478PTMScan® Pilot Phospho-Tyrosine Rabbit mAb (P-Tyr-1000)TrypsinPhosphorylationMouse Liver | XLS | RAW 1 2
5567PTMScan® Phospho-T*PP Motif (T*PP) XP® KitTrypsinPhosphorylationMouse Liver
XLS  |  RAW 1 2
5566PTMScan® Phospho-ST*P Motif (ST*P) XP® KitTrypsinPhosphorylationMouse Liver
XLS  |  RAW 1 2
13764PTMScan® Succinyl-Lysine Motif [Succ-K] KitTrypsinSuccinylationMouse Liver
XLS  |  RAW 1 2
5562PTMScan® Ubiquitin Remnant Motif (K-ε-GG) KitTrypsinUbiquitinMouse Liver
XLS  |  RAW 1 2
14482PTMScan® Pilot Ubiquitin Remnant Motif (K-ε-GG) KitTrypsinUbiquitinMouse Liver
XLS  |  RAW 1 2
32948PTMScan® Basophilic Kinase Substrate Motif [(R/X)(R/X)Xp(S/T)] KitLysC*PhosphorylationMouse Liver
XLS  |  RAW 1 2
28303PTMScan® Phospho-Ser/Thr-Pro Motif [p(S/T)P] KitTrypsinPhosphorylationMouse Liver
XLS  |  RAW 1 2
25081PTMScan® Phospho-Ser/Thr Motif [pS/T] KitLysC*PhosphorylationMouse Liver
XLS  |  RAW 1 2
17848PTMScan® Propionyl-Lysine [Prop-K] KitTrypsinPropionylationMouse Liver
XLS  |  RAW 1 2
26101PTMScan® Glutaryl-Lysine [Glut-K] KitTrypsinGlutarylationMouse Liver
XLS  |  RAW 1 2
93872PTMScan® Malonyl-Lysine [Mal-K] KitTrypsinMalonylationMouse Liver
XLS  |  RAW 1 2
10821PTMScan® Phospho-PDK1 Docking Motif [(F/Y)p(S/T)(F/Y)] KitTrypsinPhosphorylationMouse Liver
XLS  |  RAW 1 2
91336PTMScan® Phospho-Thr-X-Arg Motif [pT(P/X)R] KitTrypsinPhosphorylationHT29 Cells
XLS
71652PTMScan® Phospho-PKC Substrate Motif [(R/K)(R/K)Xp(S/T)X(R/X)] KitLysC*PhosphorylationMouse Liver
XLS  |  RAW 1 2
Mouse Liver [pT(P/X)R]
XLS  |  RAW 1 2
41343PTMScan® Anti-Biotin Kit TrypsinBiotinylationβ2AR-APEX 293 cells
XLS  |  RAW 1 2 3 4

* Recommended to perform a secondary trypsin digest post-IP