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Changing the primary antibody dilution and incubation conditions affects fluorescence intensity and signal-to-noise ratio in IF experiments.
An outline and examples of the steps performed at Cell Signaling Technology to validate target specificity of an antibody for use in immunohistochemical analysis.
Learn how to visualize protein expression in tissue with immunohistochemistry (IHC). See examples of successfully optimized IHC assays.
For years, the TME could only be viewed in single-cell snapshots by flow cytometry or limited tissue staining. IMC offers a panorama of this dynamic world.
Streamline your oncology therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.
Organelle markers for immunofluorescence analysis from CST Cell Signaling Technology
The ESC and Lineage Markers diagram provides an overview of ESC differentiation along lineage-specific pathways and links to products from CST.
Custom-build antibody conjugates that match your assay requirements and deliver superior performance, and choose from a broad catalog of labels.
Antibodies that recognize peptide motifs in the phosphorylated state are valuable research tools in western blotting. Learn how to use them in this post.
Autophagy helps normal cells maintain homeostasis and acts as a survival mechanism in response to stress, where ULK1 plays an important role in signaling.
Use our protocol compatibility table to understand which immune signaling and phenotyping antibodies will work together in your multicolor flow cytometry experiment.