Technical Support Articles
Results (709)
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic deterg...
How should I resuspend and aliquot the PTMScan® LysC Protease #84748?
To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, ...
How are the SignalStar™ Multiplex IHC Kits & Reagents validated?
CST thoroughly validates each antibody available in the The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers: Yes, please select “Human” in the first step of the SignalStar Multiplex IH... Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&a... The Concanavalin A beads can clump together rather easily, especially when the cells become lysed. To avoid this, make sure your cells are healthy and be sure to treat them very ge... CST routinely tests antibodies in relevant applications. Those that pass rigorous application-specific validation standards are recommended. We only recommend applications that hav... We have several antibodies for detecting Akt phosphorylation at Ser473 that are validated for IHC-P with human samples. These are:
CST antibodies undergo thorough validation processes to ensure reliable performance for every approved application. This includes testing different protocol variations to identify ... The SignalStar Multiplex IHC kits and reagents have been optimized with respect to fluorophore pairing and order of antibodies. As tissues vary in quality and expression level of b... We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include: The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic e... We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with human samples. These are:
The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 h... We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with mouse samples. These are:
We do not observe any bias toward euchromatin or heterochromatin in our assays. In CUT&RUN, the target-specific antibody recruits the pAG-MNase and directs digestion to chromat... Our CUT&RUN Assay Kit is optimized for use with whole cells and works with a large number of cell lines, both adherent and suspension. While it is not typically necessary to pr... For custom (carrier-free) orders, many antibodies are available pre-formulated in PBS without BSA or glycerol. This carrier-free formulation is amenable to conjugation and can be c...
Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?
PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure...
Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?
Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??
Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&RUN experiments?
How does the specificity of the F(ab’)2 fragments differ from the corresponding full-length monoclonal antibodies?
Does this antibody work for my application (western blot, immunoprecipitation, immunofluorescence, immunohistochemistry, ELISA, flow cytometry, chromatin immunoprecipitation, etc.)?
Which phospho-Akt (Ser473) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
Can I modify the CST-recommended protocol for a given application?
Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?
Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?
Can I reuse PTMScan® antibody beads?
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
Does CUT&RUN show a bias toward euchromatin or heterochromatin?
Does your CUT&RUN Assay Kit work with isolated nuclei?
Can I order a custom formulation of an antibody?
In terms of cytokine activity, what is the difference between International Units (IU) and specific activity?