Technical Support Articles
Results (709)
Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Mouse” in the first step of the SignalStar Multiplex IH...
My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?
Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are st...
What is an appropriate positive control to include in this SignalStar™ assay? Are multiple controls necessary?
Any tissue shown to be positive for each marker via chromogenic IHC can serve as a positive control tissue for a SignalStar™ assay. Each target will therefore require a positive co...
Can I autoclave the 6-Tube Magnetic Separation Rack #7017?
No, these racks cannot be autoclaved. Exposing them to high autoclave temperatures may cause the magnets to lose their magnetism. Instead, we suggest submerging or spraying the ...
Does the CUT&Tag assay show a bias toward euchromatin or heterochromatin?
With the appropriate salt concentration in the tagmention buffer, we don't observe bias toward euchromatin or heterochromatin in our assays. In CUT&Tag, the target-specific...
Why is the PTMScan® LysC Protease #84748 digestion performed at room temperature and not at 37°C?
The digestion is performed at room temperature to minimize carbamylation (also known as carbamoylation) of the sample peptides in the urea buffer, which can interfere with downstre...
Why does the material in my new vial of Echinomycin #51434 look different?
Echinomycin #51434 is lyophilized into the vial, and any difference in the appearance of the lyophilized echinomycin is due to a dry coating of the compound in the vial. When you a...
Which phospho-S6 ribosomal protein (Ser235/236) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
We have several antibodies for detecting the phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 that are validated for IF-IC with human samples. These are as follows:
What advice do you have for collecting samples of adherent cell lines versus suspension cell lines for CUT&Tag experiments?
For CUT&Tag assays using adherent cell lines, the first step is to detach the cells from the dish. We recommend using trypsin to detach the cells. Alternatively, Accutase Cell ...
What is the most suitable control DNA sample for my CUT&RUN NG-seq experiment?
When performing CUT&RUN with downstream NG-seq analysis, one can use a normal IgG antibody enriched sample as the negative control. However, we have seen and heard from other s...
Which total Akt antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) with mouse samples?
We have several total Akt antibodies that are validated for IF-IC with mouse samples. These are:
- Akt (pan) (C67E7) Rabbit mAb #4691
- Akt (pan) (11E7) Rabbit mAb #468...
How do I avoid batch effects and what sample run order should I use for PTMScan® experiments?
To avoid intra-sample batch effects during liquid chromatography with tandem mass spectrometry (LC-MS/MS), we recommend a run order that involves running one technical replicate of...
What is the expected signal strength for the PTMScan® Control peptides?
We have observed >1E6 peak area for each control peptide when used at the supplied concentration followed by PTMScan and PTMScan HS enrichment...
Which p44/42 MAPK (Erk1/2) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with mouse samples?
We have several monoclonal antibodies for studying p44 and p42 mitogen-activated protein kinases (MAPKs), also referred to as Erk2 and Erk1, that are validated in IF-IC with mouse ...
Is there a method I can use to quantify the amount of protein in my lysate after adding SDS loading buffer?
We use the Pierce 660nm Protein Assay Kit supplemented with the ionic detergent compatibility reagent (IDCR) to determine the protein concentration of lysates prepared in 1X SDS + ...
Do you have a methylene blue dot blot staining protocol?
We have found that the methylene blue staining of the amounts of DNA spotted when performing a DNA dot blot can be tricky and may need optimization.
1. It is very important ...
Does your Cas9 (7A9-3A3) Mouse mAb #14697 detect the D10A/H840A mutant (dCas9) and the D10A mutant (niCas9)?
Our Cas9 (7A9-3A3) Mouse mAb #14697 was produced with a recombinant protein specific to the amino terminus of Cas9 from Streptococcus pyogenes (UniProt ID #Q99ZW2; https://w...
Can CUT&RUN enriched DNA be analyzed by qPCR, or do I have to do NGseq to analyze my data?
Our CUT&RUN Assay Kit #86652 is compatible with downstream q...
What is the expected size distribution of CUT&RUN DNA?
When using antibodies for CUT&RUN against histone modifications, we typically see fragment sizes as small as 150 bp (size of a mono-nucleosome) and quite often see a nucleosoma...
How much CUT&RUN DNA is required for NG-seq analysis?
The amount of CUT&RUN DNA required for NG-seq analysis depends on the sensitivity of the DNA library prep kit. The typical yield for CUT&RUN DNA is 0.6 to 6 ng per reaction...
Can I use PTMScan® LysC Protease #84748 for tandem mass tag (TMT) labeling?
We have used LysC in conjunction with trypsin for TMT-labeling-based studies to reduce miscleavages. For TMT-based workflows, we recommend a higher LysC:substrate ratio of 1:100 co...
Which total Akt antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several monoclonal antibodies for detecting total Akt protein that are validated for IHC-P with mouse samples. These are as follows:
- Akt (pan) (C67E7) Rabbit mAb #...
What are the specifications for the 96-well plate in the CST ELISA kits?
For the 96-well colorimetric PathScan® and FastScan® ELISA plates, the specification information can be found in the We have had customers perform this assay using frozen cell pellets, prior to adding any fractionation buffer, and the subsequent fractionation worked fine. Once you start fracti... Smad8 and Smad9 are often used interchangeably in the literature to refer to the same protein. However, the official name of the protein is Smad9. Moreover, there ... The suggested protocol for treating cells with poly(dA:dT) can be found below: The composition of our DNA Elution Buffer #10009 is 10 mM Tris-Cl, pH 8.5. We have several antibodies for detecting ribosomal protein S6 (rpS6) that are validated for IF-IC with mouse samples. These are S6 Ribosomal Protein (5G10) Rabbit mAb #2217 and S6 ... We have several antibodies for detecting the phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 that are validated for IHC-P with human samples. These are Phospho-S6 Ribo... We have several antibodies for detecting the phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 that are validated for IHC-P with human samples. These are S6 Ribosomal Pr...
Can the Cell Fractionation Kit #9038 be used for frozen cell pellets?
Are Smad8 and Smad9 the same protein?
Does CST have a protocol for treating cells with poly(dA:dT)?
Poly(dA:dT) Treatment Protocol (5 μg/mL final concentra...
What is the composition of the DNA Elution Buffer #10009?
Which S6 ribosomal protein antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) with mouse samples?
Which phospho-S6 ribosomal protein (Ser235/236) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
Which S6 ribosomal protein antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?