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Results (694)

Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?

Yes, please select “Human” in the first step of the SignalStar Multiplex IHC Panel Builder ...

How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?

We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reagents into single use ...

Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?

The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:

PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2B

PMID: 249...

Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??

Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77...

How should I resuspend and aliquot the PTMScan® LysC Protease #84748?

To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, spin the vial at 1,0...

Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?

The SignalStar Multiplex IHC kits and reagents have been optimized with respect to fluorophore pairing and order of antibodies. As tissues vary in quality and expression level of biomarkers, increasin...

How are the SignalStar™ Multiplex IHC Kits & Reagents validated?

CST thoroughly validates each antibody available in the

Can I modify the CST-recommended protocol for a given application?

CST antibodies undergo thorough validation processes to ensure reliable performance for every approved application. This includes testing different protocol variations to identify the most effective o...

Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?

The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 has been tested succe...

Can I reuse PTMScan® antibody beads?

The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic elution step, which l...

Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&RUN experiments?

The Concanavalin A beads can clump together rather easily, especially when the cells become lysed. To avoid this, make sure your cells are healthy and be sure to treat them very gently during the cell...

Why do I need to use the three buffers in the Cell Fractionation Kit #9038?

Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p...

How do the Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 and the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 compare in terms of their sensitivity and specificity?

The Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 is a mouse mAb from a single clone, whereas the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 is a mix of rabbit mAbs from multiple clon...

In terms of cytokine activity, what is the difference between International Units (IU) and specific activity?

International Units (IU) and specific activity are two different ways of measuring...

Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?

We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with human samples. These are:

  • Cleaved Caspase-3 (Asp175) Antibody #9661
  • Cleaved ...

    My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?

    Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex...

    Does this antibody work for my application (western blot, immunoprecipitation, immunofluorescence, immunohistochemistry, ELISA, flow cytometry, chromatin immunoprecipitation, etc.)?

    CST routinely tests antibodies in relevant applications. Those that pass rigorous application-specific validation standards are recommended. We only recommend applications that have passed rigorous va...

    Which total Akt antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?

    We have several monoclonal antibodies for detecting total Akt protein that are validated for IHC-P with mouse samples. These are as follows: 

    • Akt (pan) (C67E7) Rabbit mAb #4691
    • Akt (pa...

      Why can't I add a target to the channel I want when using the SignalStar™ Multiplex IHC Panel Builder?

      During our extensive validation process, we found that some targets do not perform optimally with specific fluorophore pairings. When this is the case, the SignalStar Multiplex IHC Panel Builder will ...

      Which total Akt antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?

      We have several monoclonal antibodies for detecting total Akt protein that are validated for IHC-P in human samples. These are as follows: 

      • Akt (pan) (C67E7) Rabbit mAb #4691
      • Akt (pan)...

        Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?

        We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with mouse samples. These are:

        • Cleaved Caspase-3 (Asp175) Antibody #9661
        • Cleaved ...

          What are the specifications for the 96-well plate in the CST ELISA kits?

          For the 96-well colorimetric PathScan® and FastScan® ELISA plates, the specification information can be found in the 

          Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?

          We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include: 

          • β-Actin (13E5) Rabbit mAb #4970
          • β-Actin (D6A8) Rabbit mAb #...

            What advice do you have for collecting samples of adherent cell lines versus suspension cell lines for CUT&Tag experiments?

            For CUT&Tag assays using adherent cell lines, the first step is to detach the cells from the dish. We recommend using trypsin to detach the cells. Alternatively, Accutase Cell Dissociation Reagent...

            How does the specificity of the F(ab’)2 fragments differ from the corresponding full-length monoclonal antibodies?

            Since the paratopes of the antibody fragments are identical to that of the corresponding full-length monoclonal antibodies, their specificity for scFv linkers remains unchanged. However, the abs...

            Why is the PTMScan® LysC Protease #84748 digestion performed at room temperature and not at 37°C?

            The digestion is performed at room temperature to minimize carbamylation (also known as carbamoylation) of the sample peptides in the urea buffer, which can interfere with downstream PTMScan enrichmen...

            Can I order a custom formulation of an antibody?

            For custom (carrier-free) orders, many antibodies are available pre-formulated in PBS without BSA or glycerol. This carrier-free formulation is amenable to conjugation and can be conjugated to fluorop...

            Why is the concentration of my antibody so low?

            At CST, our antibodies are formulated on the basis of their reactivity and performance for each approved application at a recommended dilution. For antibodies with high affinity, optimal performan...

            Can I use PTMScan® LysC Protease #84748 for tandem mass tag (TMT) labeling?

            We have used LysC in conjunction with trypsin for TMT-labeling-based studies to reduce miscleavages. For TMT-based workflows, we recommend a higher LysC:substrate ratio of 1:100 compared to the 1:250 ...

            Can CUT&Tag-enriched DNA be analyzed by qPCR, or do I have to do NGS to analyze my data?

            If qPCR analysis is desired, we recommend performing CUT&RUN, as CUT&Tag DNA is not compatible with qPCR. The sample incubation step at 58℃ required for tagmented DNA solubilization in the ...