Technical Support Articles
Results (713)
What is the intended use of the PTMScan® Control peptides?
These synthetic peptides are recoverable using our immunoaffinity purification (IAP) antibody beads, and thus provide users with a measure of PTM...
What is the expected yield of a CUT&Tag DNA library?
The expected yield of an amplified CUT&Tag DNA library depends on the DNA quantification system used.
- If you are using a NanoDrop Spectrophotometer or QIAxpert System, ...
Can I use the Cyclic AMP XP® Assay Kit #4339 with tissue samples?
We have not tested tissue samples in-house using the Cyclic AMP XP Assay Kit #4339, so although we cannot guarantee it will work, there is no reason it should not. We recommend loa...
Can I use your Protein A Agarose Beads #9863 and Protein G Agarose Beads #37478 if they have been frozen?
Although we are unable to guarantee that both our Protein A Agarose Beads #9863 and Protein G Agarose Beads #37478 will work after being stored at -20C, we have used beads that hav...
Can you provide data that confirms the specificity of your BACE (D10E5) Rabbit mAb #5606?
The specificity of our BACE (D10E5) Rabbit mAb #5606 has been confirmed in the literature on brain extracts prepared from BACE1 (-/-) mice. Please see Figure 1 in Shahani, N. et al...
Can I use RIPA Buffer (10X) #9806 for ELISA experiments?
Our ELISA kits are compatible with our RIPA Buffer (10X) #9806, however, this bu...
I don’t see my target of interest in your menu of available antibodies for SignalStar™ Multiplex IHC. Can I still use it in my panel in some way?
SignalStar Multiplex IHC kits and reagents haven’t yet been validated for use with antibodies outside of our menu. We’re in the proces...
How long after the completion of staining can I wait to image my slides when performing a SignalStar™ Multiplex IHC assay?
For Imaging Round 1, the staining should show robust signal when imaged up to 8 hours post completion of staining. For Imaging Round 2, imaging should be performed as close to the ...
What are the high molecular weight bands detected by the β1-Adrenergic Receptor Antibody #12271?
The antigen for the β1-Adrenergic Receptor Antibody #12271 surrounds Gly406 of the human β1-Adrenergic Receptor protein and is contained entirely in the cytoplasmic domain. #12271 ...
Will your Anti-biotin, HRP-linked Antibody #7075 cross-react with endogenous biotin in cell lysates?
Our Anti-biotin, HRP-linked Antibody #7075 is intended for use with our Biotinylated Protein Ladder Detection Pack #7727. However, it is possible for this antibody to also detec...
What samples and species were tested with the Griess Reagent Nitrite Measurement Kit #13547?
We have tested the Griess Reagent Nitrite Measurement Kit #13547 with media, cell culture supernatant, and urine. It should cross-react with all species types.
Where can I find the concentration of my antibody?
For a recently purchased antibody, the concentration will be found either printed directly on the vial label or in the Certificate of Analysis (CoA). CoAs for the current shipping ...
What are the differences between the BrdU Cell Proliferation Chemiluminescent Assay Kit #5492 and the BrdU Cell Proliferation Assay Kit #6813? Which should I choose?
In general, the BrdU Cell Proliferation Chemiluminescent Assay Kit #5492 offers a broader dynamic range and higher sensitivity compared to the BrdU Cell Proliferation Assay Kit #68...
Are the Protein A Magnetic Beads #73778 and the Protein G Magnetic Beads #70024 hydrophilic or hydrophobic?
The base particle of the Protein A Magnetic Beads #73778 and the Protein G Magnetic Beads #70024 is somewhat hydrophilic. It is difficult to predict the hydrophilicity or hydrophob...
Do the recombinant SARS-CoV-2 spike proteins from CST have the same folding as the native SARS-CoV-2 spike protein?
Unfortunately, we cannot confirm that the recombinant SARS-CoV-2 spike proteins we provide are in exactly the same conformation that would be expected in nature, as we do not yet h...
Does Cell Lysis Buffer (10X) #9803 work with the Bradford assay?
Cell Lysis Buffer (10X) #9803 will interfere with the Bradford protein assay. This is because it contains detergent. To make the assay somewhat compatible, dilute your protein quan...
Can you help me calculate the bioactivity of my cytokine?
Specific activity (expressed in units/mg) is a function of cytokine or growth factor purity and appropriate protein folding. To determine the specific activity, typically the reado...
Can I purchase your Sample Normalization Spike-In DNA (2 pg/µl) #29987 as a stand-alone product?
Unfortunately, we are unable to provide the Sample Normalization Spike-In DNA (2 pg/µl) #29987 as a stand-alone product. This product is m...
What PD-L2 antibodies do you currently offer?
We currently offer three products targeting PD-L2:
Our PD-L2 (D7U8C) XP® Rabbit mAb #82723 recognizes endogenous levels of total human PD-L2 protein and was produced by immu...
Does your Anti-mouse IgG, HRP-linked Antibody #7076 cross react with mouse IgM?
Our Anti-mouse IgG, HRP-linked Antibody #7076 shows approximately 25% reactivity to mouse IgM.
Can I combine antibodies used in a SignalStar™ assay with direct conjugates?
SignalStar Multiplex IHC kits and reagents have been validated for use in combination with direct conjugates. The SignalStar™ Fluorescence Removal Kit Our CUT&Tag kit is optimized for use with whole cells and works with a large number of cell lines, both adherent and suspension. While our kit works with pre-isolated nuclei be... PTMScan® is compatible with the use of stable isotope labeling using amino acids in cell culture, or SILAC. The incorporation of heavy stable iso... Protein A predominantly binds intact IgG and does not bind denatured IgG well. It can be used in IP experiments to help avoid the potential masking of signal by denatured IgG heavy... When performing In-Cell Western assays with DyLight 680-conjugated secondary antibodies, we recommend using either Hoechst 33342 or DAPI as a nuclear dye. DRAQ5 cannot be used beca... SignalStar™ Multiplex IHC Kits & Reagents have not been validated for use in combination with other assays. The SignalStar technology does not destroy the tissue, so it may be ... Due to the nature of the Cell Fractionation Kit #9038 and the localization of each of the proteins in the Loading Control Sampler Kit #5142, the proteins will not be equally expres... We have shown that our CUT&RUN Assay Kit #86652 works with as few as 5,000-10... The amount of CUT&RUN DNA required for NG-seq analysis depends on the sensitivity of the DNA library prep kit. The typical yield for CUT&RUN DNA is 0.6 to 6 ng per reaction... We have had customers perform this assay using frozen cell pellets, prior to adding any fractionation buffer, and the subsequent fractionation worked fine. Once you start fracti...
Does the CST CUT&Tag kit work with isolated nuclei?
Is PTMScan® compatible with SILAC?
Why should I use the Protein A (HRP Conjugate) #12291 in place of the secondary antibody in my western blot for IP experiments?
Which nuclear dyes can be used for In-Cell Western assays in combination with DyLight 680-conjugated secondary antibodies?
Can my slides be used for other assays after SignalStar™ Multiplex IHC?
What can I use as a loading control with the Cell Fractionation Kit #9038?
What is the fewest number of cells I can use in a reaction with your CUT&RUN Assay Kit #86652?
How much CUT&RUN DNA is required for NG-seq analysis?
Can the Cell Fractionation Kit #9038 be used for frozen cell pellets?