Technical Support Articles
Results (689)
Does the Human IL-22 Recombinant Protein #74808 contain any human or animal material and is it free of HIV/AIDS, HCV/HBC or other infectious material?
No materials of animal origin are used in the production of our Human IL-22 Recombinant Protein #74808. Therefore, additional testing for infectious material is not relevant.
Can I use your Protein A Agarose Beads #9863 and Protein G Agarose Beads #37478 if they have been frozen?
Although we are unable to guarantee that both our Protein A Agarose Beads #9863 and Protein G Agarose Beads #37478 will work after being stored at -20C, we have used beads that have been frozen and th...
Can I use mixed species samples for a PTMScan® experiment?
The PTMScan® technology is compatible with samples derived from all kingdoms of life as well as samples containing mixed species like xenografts (mouse and human), microbiome samples (bacterial and ho...
Can I reuse PTMScan® antibody beads?
The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic elution step, which l...
How are the SignalStar™ Multiplex IHC Kits & Reagents validated?
CST thoroughly validates each antibody available in the Yes, the lyophilized PTMScan® LysC Protease #84748 may be stored at -80°C. However, we do not recommend storing the LysC powder in a frost-free freezer or a freezer that undergoes freeze-thaw cycles. ... We have several antibodies for detecting total Akt protein that are validated for IF-IC with human samples. These are as follows:
Can I store the unopened, lyophilized PTMScan® LysC Protease #84748 at -80°C instead of -20°C?
Which total Akt antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
Will the Non-phospho (Active) YAP (Ser127) (E6U8Z) Rabbit mAb #29495 cross-react with TAZ?
YAP and TAZ are co-expressed abundantly in every cell line that has been shown to be positive for either protein, and likewise, they are negative/low in the same cell lines (essentially, they appear t...
How many assays can be performed with the Senescence β-Galactosidase Staining Kit #9860?
The reagents provided with our Senescence β-Galactosidase Staining Kit #9860 are sufficient to stain 125 x 35 mm wells. The volumes listed in our protocol are intended for one 35 mm well in a 6-we...
Can you share your protocol for preparing Jurkat -/+ Anti-CD3 antibody (10 µg/mL for 2 min) cell extracts?
We prepare Jurkat -/+ Anti-CD3 antibody (10 µg/mL for 2 min) cell extracts as follows:
- Jurkat cells are cultured to the desired volume (200-400 mL) and a density of approximately 1x10...
Can I freeze the PTMScan® HS Magnetic Immunoaffinity Beads?
The PTMScan HS Magnetic Immunoaffinity Beads should not be frozen. We recommend storing this product at 4C. However, we have performed functional testing by IAP and subsequent mass spec analysis after...
Why does your AMPKα Antibody #2532 detect bands at approximately 50 kDa?
While we know from our experiments that our AMPKα Antibody #2532 can detect both the alpha1 and alpha2 isoforms of the catalytic subunit, these should both run above 60kDa. We do occasionally see some...
Why doesn't PD-L2 migrate at 31 kDa?
The predicted molecular weight of PD-L2 is 31 kDa (based on amino acid composition). However, in most models, PD-L2 migrates at an observed molecular weight of 45-60 kDa on Tris-Glycine gels. This dis...
What is the minimal amount of tissue required for a CUT&RUN experiment?
While we recommend 1mg of tissue per assay in most cases, we have had some success with as little as 0.5 mg of tissue per assay. Easier tissue types, such as liver, or easier target types, such as his...
How long can I leave glycerol on my plates for the Senescence β-Galactosidase Staining Kit #9860?
From our limited in-house testing, we would suggest leaving glycerol on the plates for 3-5 days at most. We have not tested longer time periods than this.
What is the composition of the DNA Elution Buffer #10009?
The composition of our DNA Elution Buffer #10009 is 10 mM Tris-Cl, pH 8.5.
How many LC-MS/MS injections are obtained from a PTMScan® enrichment?
Each PTMScan® enrichment contains sufficient peptides for three to four LC-MS/MS runs. Therefore, one enrichment can produce enough peptides for technical replicates and enough remaining peptides for ...
Does your Ki-67 (D2H10) Rabbit mAb #9027 cross-react with mouse tissue by IHC?
We have tested the Ki-67 (D2H10) Rabbit mAb #9027 on a variety of FFPE mouse tissues and have not observed specific staining. Based on this, we have concluded that #9027 does not detect mouse Ki-67, h...
Why should I use your recommended protocol or reagents for my IHC experiment?
We have performed a number of comparisons over the years looking at protocol variables such as the retrieval solution, diluent, incubation, and detection methodology. We have seen that the choice of r...
Will the RAG1 (D36B3) Rabbit mAb #3968 cross-react with human RAG2?
Our RAG1 (D36B3) Rabbit mAb #3968 was produced using a recombinant protein fragment with a sequence toward the N-terminal region of human RAG1. No significant homology is found in this region when ali...
Are the light chains of the β-Actin (8H10D10) Mouse mAb #3700 kappa or lambda?
The β-Actin (8H10D10) Mouse mAb #3700 has kappa light chains.
Why are there two non-reduced bands in the gel image for your Human ACE2 (18-652) Recombinant Protein (mFc-Tag) #83986?
The top band in the gel image for our Human ACE2 (18-652) Recombinant Protein (mFc-Tag) #83986 likely represents dimerization due to the mFc tag.
Why do I see a doublet when using my total Histone H3 antibody for western blot?
We see this doublet phenomenon often in a number of different cell lines that we test with our Histone H3 antibodies. It does appear to be sample dependent. The doublet may be due to a proteolytic fra...
Does your Tau (D1M9X) XP® Rabbit mAb #46687 recognize all six Tau isoforms?
Our Tau (D1M9X) XP® Rabbit mAb #46687 is predicted to recognize 2N4R (tau40), 2N3R (tau39), 1N4R (tau46), 1N3R (tau37), 0N4R (tau24), and 0N3R (tau23), as all six isoforms include the antigenic peptid...
Is TMT labeling compatible with PTMScan®?
We understand some customers choose to pursue TMT labeling for PTMScan proteomics analysis, but we do not have a defined protocol for this method. However, there are two questions we can address:<...
Why does MAFA (D2Z6N) Rabbit mAb #79737 recognize a band at approximately 48 kDa when the predicted molecular weight is approximately 37 kDa?
MAFA is a phosphoprotein. The multiple phosphorylation sites on this protein cause a mobility shift, resulting in the protein migrating slower by gel electrophoresis. This has been confirmed by treatm...
What is the expected CUT&RUN DNA yield when starting with extremely low cell numbers, such as 5,000 - 10,000 cells?
If you start with 100,000 cells, you typically get 0.6-6ng DNA. However, if you start with 5,000 - 10,000 cells, the DNA yield can be as low as 0.1-0.2 ng or even undetectable by the picogreen assay. ...
Why is it important to include both positive and negative controls in your IF experiment?
Positive and negative controls are at the heart of any good experiment. Without them, it is difficult to assess root causes when troubleshooting. Positive controls should be included to demonstrate: <...
Can your Streptavidin-HRP #3999 be used for ELISA?
Regrettably, we do not test our Streptavidin-HRP #3999 for use in ELISA . Therefore, we cannot provide any firm guarantees or a recommended dilution. However, it should work for ELISA with optimizatio...
I don’t see my target of interest in your menu of available antibodies for SignalStar™ Multiplex IHC. Can I still use it in my panel in some way?
SignalStar Multiplex IHC kits and reagents haven’t yet been validated for use with antibodies outside of our menu. We’re in the process of developing cust...