Technical Support Articles
Results (713)
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic deterg...
How should I resuspend and aliquot the PTMScan® LysC Protease #84748?
To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, ...
Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Human” in the first step of the SignalStar Multiplex IH...
Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?
The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:
PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure...
Can I order a custom formulation of an antibody?
For custom (carrier-free) orders, many antibodies are available pre-formulated in PBS without BSA or glycerol. This carrier-free formulation is amenable to conjugation and can be c...
How can I confirm the PTMScan® LysC Protease #84748 digested my proteins efficiently?
To confirm digestion, we recommend taking a ~1% aliquot of the protein sample pre- and post-digestion and running them on an SDS-PAGE gel with Coomassie blue staining. After di...
Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&RUN experiments?
The Concanavalin A beads can clump together rather easily, especially when the cells become lysed. To avoid this, make sure your cells are healthy and be sure to treat them very ge...
Can I store the unopened, lyophilized PTMScan® LysC Protease #84748 at -80°C instead of -20°C?
Yes, the lyophilized PTMScan® LysC Protease #84748 may be stored at -80°C. However, we do not recommend storing the LysC powder in a frost-free freezer or a freezer that undergoes ...
Can CST develop an antibody for me?
CST does not develop custom antibodies. However, we are happy to accept your target suggestions. Please CST routinely tests antibodies in relevant applications. Those that pass rigorous application-specific validation standards are recommended. We only recommend applications that hav... Our CUT&RUN Assay Kit is optimized for use with whole cells and works with a large number of cell lines, both adherent and suspension. While it is not typically necessary to pr... We have several antibodies for detecting Akt phosphorylation at Ser473 that are validated for IF-IC with mouse tissues. These are: Yes, please select “Mouse” in the first step of the SignalStar Multiplex IH... These synthetic peptides are recoverable using our immunoaffinity purification (IAP) antibody beads, and thus provide users with a measure of PTM... Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are st... ZBP1 (Z-DNA binding protein 1), also referred to as DAI (DNA-dependent activator of IFN-regulatory factors) and DLM-1, is a nucleotide-binding protein that plays a role in tumorige... SignalStar Multiplex IHC kits and reagents haven’t yet been validated for use with antibodies outside of our menu. We’re in the proces... During our extensive validation process, we found that some targets do not perform optimally with specific fluorophore pairings. When this is the case, the SignalStar Multiplex IHC... SignalStar Multiplex IHC kits and reagents have been validated for use in combination with direct conjugates. The SignalStar™ Fluorescence Removal Kit We have found 100,000 cells per assay to be sufficient for enriching target loci for multiple histone modifications, transcription factors, and transcription cofactors. However, on... When selecting an antibody for use in CUT&RUN, we recommend starting with a ChIP or ChIP-seq-validated antibody; however, we have found that not all ChIP-validated antibodie... We have several antibodies for detecting cleaved PARP that are validated for IF-IC with human samples. These are Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625 and Cleaved-PAR... During the course of optimization, we’ve found that fluorescent staining may show higher %-positivity than chromogenic staining. To ensure any excess staining is specific, confirm ... Our Ras (G12D Mutant Specific) (D8H7) Rabbit mAb #14429 and Ras (G12V Mutant Specific) (D2H12) Rabbit mAb #14412 will detect all three types of Ras (H-Ras, K-Ras, and N-Ras) if the... Any tissue shown to be positive for each marker via chromogenic IHC can serve as a positive control tissue for a SignalStar™ assay. Each target will therefore require a positive co... We have several monoclonal antibodies for detecting p44 and p42 MAPK when phosphorylated at Thr202 and Tyr204 of Erk1, or Thr185 and Tyr187 of Erk2, that are validated for IF-IC wi... Possibly. CST scientists test all our antibodies on multiple species when feasible, depending on the target protein and the experimental systems available. If a tested species does... The most sensitive antibody will depend on whether you are trying to detect Cas9 protein from Streptococcus pyogenes, Staphylococcus aureus, or Campylobacter jejun... SignalStar™ Multiplex IHC Kits & Reagents have not been validated for use in combination with other assays. The SignalStar technology does not destroy the tissue, so it may be ...
Does this antibody work for my application (western blot, immunoprecipitation, immunofluorescence, immunohistochemistry, ELISA, flow cytometry, chromatin immunoprecipitation, etc.)?
Does your CUT&RUN Assay Kit work with isolated nuclei?
Is Fc blocking needed for F(ab') antibody fragments used in live cell flow cytometry assays?
Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) with mouse samples?
Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?
What is the intended use of the PTMScan® Control peptides?
My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?
What isoforms does the ZBP1 Antibody #60968 recognize?
I don’t see my target of interest in your menu of available antibodies for SignalStar™ Multiplex IHC. Can I still use it in my panel in some way?
Why can't I add a target to the channel I want when using the SignalStar™ Multiplex IHC Panel Builder?
Can I combine antibodies used in a SignalStar™ assay with direct conjugates?
Is there a maximum number of cells that can be used in a single CUT&RUN assay?
What is the best way to choose an antibody for my CUT&RUN assay if I cannot find a CUT&RUN validated antibody for my target protein?
Which cleaved PARP antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) in human samples?
When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. How do I know if this excess staining is correct?
What types of Ras are recognized by your G12D and G12V mutant specific antibodies?
What is an appropriate positive control to include in this SignalStar™ assay? Are multiple controls necessary?
Which Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with mouse samples?
Have species been tested if they are not listed as cross-reactive with a CST antibody on the datasheet?
Which Cas9 antibody is the most sensitive by western immunoblot?
Can my slides be used for other assays after SignalStar™ Multiplex IHC?