Technical Support Articles
Results (713)
What is the solution formulation of the PTMScan Control peptides?
The solution formulation of the PTMScan Control peptides is 25% acetonitrile, 74.9% water, 0.1% trifluoroacetic acid (V:V).<...
How should I resuspend and aliquot the PTMScan® LysC Protease #84748?
To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, ...
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic deterg...
How are the SignalStar™ Multiplex IHC Kits & Reagents validated?
CST thoroughly validates each antibody available in the Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&a... CST antibodies undergo thorough validation processes to ensure reliable performance for every approved application. This includes testing different protocol variations to identify ... The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers: We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reage... The SignalStar Multiplex IHC kits and reagents have been optimized with respect to fluorophore pairing and order of antibodies. As tissues vary in quality and expression level of b... Our CUT&RUN Assay Kit #86652 is compatible with downstream q... The Concanavalin A beads can clump together rather easily, especially when the cells become lysed. To avoid this, make sure your cells are healthy and be sure to treat them very ge... SignalStar Multiplex IHC kits and reagents have been validated for use in combination with direct conjugates. The SignalStar™ Fluorescence Removal Kit We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include: Possibly. CST scientists test all our antibodies on multiple species when feasible, depending on the target protein and the experimental systems available. If a tested species does... The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic e... We have several total Akt antibodies that are validated for IF-IC with mouse samples. These are:
Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??
Can I modify the CST-recommended protocol for a given application?
Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?
PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure...
How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?
Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?
Can CUT&RUN enriched DNA be analyzed by qPCR, or do I have to do NGseq to analyze my data?
Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&RUN experiments?
Can I combine antibodies used in a SignalStar™ assay with direct conjugates?
Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?
Have species been tested if they are not listed as cross-reactive with a CST antibody on the datasheet?
Can I reuse PTMScan® antibody beads?
Which total Akt antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) with mouse samples?
How do the Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 and the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 compare in terms of their sensitivity and specificity?
The Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 is a mouse mAb from a single clone, whereas the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 is a mix of rabbit mAb...
What is the expected size distribution of CUT&RUN DNA?
When using antibodies for CUT&RUN against histone modifications, we typically see fragment sizes as small as 150 bp (size of a mono-nucleosome) and quite often see a nucleosoma...
Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?
The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 h...
What is the most suitable control DNA sample for my CUT&RUN NG-seq experiment?
When performing CUT&RUN with downstream NG-seq analysis, one can use a normal IgG antibody enriched sample as the negative control. However, we have seen and heard from other s...
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with human samples. These are:
- Cleaved Caspase-3 (Asp175) Antibody #9...
Can I order a custom formulation of an antibody?
For custom (carrier-free) orders, many antibodies are available pre-formulated in PBS without BSA or glycerol. This carrier-free formulation is amenable to conjugation and can be c...
What DNA library prep kit do you recommend using for CUT&RUN?
We recommend using our SimpleChIP® ChIP-seq DNA Library Prep Kit for Illum...
Why is the concentration of my antibody so low?
At CST, our antibodies are formulated on the basis of their reactivity and performance for each approved application at a recommended dilution. For antibodies with high affinit...
Can the Cell Fractionation Kit #9038 be used for frozen cell pellets?
We have had customers perform this assay using frozen cell pellets, prior to adding any fractionation buffer, and the subsequent fractionation worked fine. Once you start fracti...
How does the specificity of the F(ab’)2 fragments differ from the corresponding full-length monoclonal antibodies?
Which total Akt antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have several monoclonal antibodies for detecting total Akt protein that are validated for IHC-P in human samples. These are as follows:
- Akt (pan) (C67E7) Rabbit mAb #46...