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How should I resuspend and aliquot the PTMScan® LysC Protease #84748?

To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, spin the vial at 1,0...

Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??

Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77...

Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?

Yes, please select “Human” in the first step of the SignalStar Multiplex IHC Panel Builder ...

Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?

The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:

PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2B

PMID: 249...

How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?

We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reagents into single use ...

Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?

The SignalStar Multiplex IHC kits and reagents have been optimized with respect to fluorophore pairing and order of antibodies. As tissues vary in quality and expression level of biomarkers, increasin...

How are the SignalStar™ Multiplex IHC Kits & Reagents validated?

CST thoroughly validates each antibody available in the

Can I modify the CST-recommended protocol for a given application?

CST antibodies undergo thorough validation processes to ensure reliable performance for every approved application. This includes testing different protocol variations to identify the most effective o...

In terms of cytokine activity, what is the difference between International Units (IU) and specific activity?

International Units (IU) and specific activity are two different ways of measuring...

Can I order a custom formulation of an antibody?

For custom (carrier-free) orders, many antibodies are available pre-formulated in PBS without BSA or glycerol. This carrier-free formulation is amenable to conjugation and can be conjugated to fluorop...

Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?

We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include: 

  • β-Actin (13E5) Rabbit mAb #4970
  • β-Actin (D6A8) Rabbit mAb #...

    Can I reuse PTMScan® antibody beads?

    The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic elution step, which l...

    Does this antibody work for my application (western blot, immunoprecipitation, immunofluorescence, immunohistochemistry, ELISA, flow cytometry, chromatin immunoprecipitation, etc.)?

    CST routinely tests antibodies in relevant applications. Those that pass rigorous application-specific validation standards are recommended. We only recommend applications that have passed rigorous va...

    Which phospho-AMPKα antibody do you suggest for my western blot experiment?

    The selection of a phospho-AMPKα antibody depends on the subunit of interest. Cell Signaling Technology has four antibodies that target phospho-AMPKα:

    • Phospho-AMPKα (Thr172) (40H9) R...

      What controls should I include when performing a Simple Western™ experiment?

      In addition to a known positive control sample, we recommend the following negative controls for your Simple Western™ experiment.

      1. A no lysate control. This includes the primary antibody at t...

      Is Fc blocking needed for F(ab') antibody fragments used in live cell flow cytometry assays?

      No, the use of Fc block is not required when using F(ab') or F(ab')2 antibody fragments in live cell flow cytometry assays, as removal of the Fc domain eliminates the interaction between...

      What isoforms does the ZBP1 Antibody #60968 recognize?

      ZBP1 (Z-DNA binding protein 1), also referred to as DAI (DNA-dependent activator of IFN-regulatory factors) and DLM-1, is a nucleotide-binding protein that plays a role in tumorigenesis and innate imm...

      Which phospho-Akt (Ser473) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?

      We have several antibodies for detecting Akt phosphorylation at Ser473 that are validated for IHC-P with human samples. These are: