Technical Support Articles
Results (690)
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with mouse samples. These are:
- Cleaved Caspase-3 (Asp175) Antibody #9661
- Cleaved ...
Have you done any cross-reactivity testing with α-CGRP, β-CGRP and unprocessed precursors using the CGRP (D5R8F) Rabbit mAb #14959?
Our CGRP (D5R8F) Rabbit mAb #14959 is produced with a synthetic peptide corresponding to residues surrounding Val114 of human CGRP protein (UniProt ID P06881 isoform 3; http://www.uniprot.org/uniprot/...
Which phospho-S6 ribosomal protein (Ser235/236) antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) with mouse samples?
We have several antibodies for detecting the phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 that are validated for IF-IC with mouse samples. These are Phospho-S6 Ribosomal Protein (Ser23...
Does your Insulin (C27C9) Rabbit mAb #3014 detect proinsulin?
Our Insulin (C27C9) Rabbit mAb #3014 was produced with an antigenic peptide corresponding to residues within the insulin B chain of the human insulin protein (UniProt ID #P01308; https://www.uniprot.o...
Which isotype control should I use for IHC?
For mouse IgGs, you will need to determine the isotype of the primary antibody and use the appropriate isotype control:
IgG1: Mouse (G3A1) mAb IgG1 Isotype Control #5415
IgG2a: Mouse (E5Y6Q) mAb...
Do I need to optimize the Tn5 tagmentation conditions for different cell types when performing CUT&Tag?
Our optimized tagmentation conditions for the CUT&Tag assay work well across multiple cell lines, both adherent and suspension. We recommend using 2 μL of CUT&Tag pAG-Tn5 (Loaded) #79561 in...
What is the benefit of carrier vs. carrier free cytokines?
Our cytokines and growth factors are lyophilized and supplied either with 20ug BSA per 1ug of cytokine (with carrier) or without added protein (carrier free). BSA minimizes adsorption to surfaces and ...
Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&Tag experiments?
Concanavalin A beads can clump together rather easily, especially when cells become lysed. Make sure your cells are healthy and be sure to treat them very gently during the cell wash. We also recommen...
Is it possible to perform CUT&Tag on frozen tissue samples?
Yes, our CUT&Tag Assay Kit works with flash frozen tissue samples or tissue samples directly frozen at -80℃. After thawing, fixation of the tissue sample is optional, as we haven't found that ...
Which phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have several antibodies for detecting p44 and p42 MAPK when phosphorylated at Thr202 and Tyr204 of Erk1, or Thr185 and Tyr187 of Erk2, that are validated for IHC-P with human samples. These are Pho...
Which beta-actin antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have several antibodies for β-actin that are validated for IHC-P in human samples. These are β-Actin (13E5) Rabbit mAb #4970 and β-Actin (8H10D10) Mouse mAb #3700. Both work well and there is no pr...
Which beta-actin antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several antibodies for β-actin that are validated for IHC-P in mouse samples. These are β-Actin (13E5) Rabbit mAb #4970 and β-Actin (8H10D10) Mouse mAb #3700. We would recommend β-Actin (13E5)...
Which phospho-S6 ribosomal protein (Ser235/236) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
We have several antibodies for detecting the phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 that are validated for IF-IC with human samples. These are as follows:
- Phospho-S6 Ribo...
Why should I include a stage tip step in my PTMScan® experiment?
For PTMScan experiments, the stage tip, or small-scale peptide desalting is a critical part of the experiment for a number of reasons. Detailed instructions for performing stage tip peptide desalting ...
Do you have a methylene blue dot blot staining protocol?
We have found that the methylene blue staining of the amounts of DNA spotted when performing a DNA dot blot can be tricky and may need optimization.
1. It is very important to perform the methy...
Do you have a recommended protocol for stripping/re-probing blots?
The western blot stripping/re-probing protocol for our Western Blot Stripping Buffer (5X) #91925 is below. However, it is important to note that we typically advise against stripping/re-probing becaus...
Can I combine antibodies used in a SignalStar™ assay with direct conjugates?
SignalStar Multiplex IHC kits and reagents have been validated for use in combination with direct conjugates. The SignalStar™ Fluorescence Removal Kit For the 96-well colorimetric PathScan® and FastScan® ELISA plates, the specification information can be found in the Human Fc-gamma Receptor 1a (FCGR1A) exhibits strong cross-reactivity with rabbit IgG, and mouse IgG2a, IgG2c, and IgG3. Of course, mouse Fc-gamma Receptors will react with mouse antibodies. In flow cy... We have several antibodies for ribosomal protein S6 (rpS6) that are validated for IHC-P with mouse samples. These are S6 Ribosomal Protein (5G10) Rabbit mAb #2217 and S6 Ribosomal Protein (54D2) Mouse... We have several monoclonal antibodies for studying p44 and p42 mitogen-activated protein kinases (MAPKs), also referred to as Erk2 and Erk1, that are validated for IHC-P with human samples. These are ... The concentration of the pAG-MNase Enzyme #57813 in our CUT&RUN kits is 25ug/ml. The recommended primary antibody dilution buffer for western blotting (i.e., BSA or nonfat dry milk) can be found either in a grey box on the bottom of the first page of the product datasheet or near ... The PTMScan® technology is compatible with samples derived from all kingdoms of life as well as samples containing mixed species like xenografts (mouse and human), microbiome samples (bacterial and ho... Since the Cell Fractionation Kit #9038 is primarily meant to be used for cultured cell lines, the buffers have not been optimized for use with tissue samples. Therefore, the fractions may not be compl... Occasionally, Cell Lysis Buffer (10X) #9803 stored at -20C may not appear frozen, which is not a reason for concern. This phenomenon is known as nucleation, or the initial formation of ice from a liqu... Our Anti-biotin, HRP-linked Antibody #7075 is intended for use with our Biotinylated Protein Ladder Detection Pack #7727. However, it is possible for this antibody to also detect endogenously bioti... Although we are unable to guarantee that both our Protein A Agarose Beads #9863 and Protein G Agarose Beads #37478 will work after being stored at -20C, we have used beads that have been frozen and th... For a recently purchased antibody, the concentration will be found either printed directly on the vial label or in the Certificate of Analysis (CoA). CoAs for the current shipping lot of many of our p... The Senescence β-Galactosidase Staining Kit #9860 is intended to be a qualitative assay to determine if cells are senescent. We have not determined a means of quantification in-house. However, we usua...What are the specifications for the 96-well plate in the CST ELISA kits?
Do I need to perform Fc blocking when using rabbit or mouse antibodies in flow cytometry?
Which ribosomal protein S6 (rpS6) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
Which p44/42 MAPK (Erk1/2) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
What is the concentration of the pAG-MNase Enzyme #57813?
Where can I find the recommended primary antibody dilution buffer for western blotting (BSA or milk)?
Can I use mixed species samples for a PTMScan® experiment?
Can I use the Cell Fractionation Kit #9038 with tissue samples?
Why isn't my bottle of Cell Lysis Buffer (10X) #9803 frozen?
Will your Anti-biotin, HRP-linked Antibody #7075 cross-react with endogenous biotin in cell lysates?
Can I use your Protein A Agarose Beads #9863 and Protein G Agarose Beads #37478 if they have been frozen?
Where can I find the concentration of my antibody?
Can I quantify my samples using the Senescence β-Galactosidase Staining Kit #9860?