Technical Support Articles
Results (694)
How should I resuspend and aliquot the PTMScan® LysC Protease #84748?
To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, spin the vial at 1,0...
Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?
The SignalStar Multiplex IHC kits and reagents have been optimized with respect to fluorophore pairing and order of antibodies. As tissues vary in quality and expression level of biomarkers, increasin...
Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?
The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:
PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2B
PMID: 249...
Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Human” in the first step of the SignalStar Multiplex IHC Panel Builder ...
How are the SignalStar™ Multiplex IHC Kits & Reagents validated?
CST thoroughly validates each antibody available in the Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p... Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77... We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reagents into single use ... Possibly. CST scientists test all our antibodies on multiple species when feasible, depending on the target protein and the experimental systems available. If a tested species does not show cross-reac... Our CUT&RUN Assay Kit #86652 is compatible with downstream qPCR analysis. The pr... The Concanavalin A beads can clump together rather easily, especially when the cells become lysed. To avoid this, make sure your cells are healthy and be sure to treat them very gently during the cell... Human Fc-gamma Receptor 1a (FCGR1A) exhibits strong cross-reactivity with rabbit IgG, and mouse IgG2a, IgG2c, and IgG3. Of course, mouse Fc-gamma Receptors will react with mouse antibodies. In flow cy... The Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 is a mouse mAb from a single clone, whereas the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 is a mix of rabbit mAbs from multiple clon... Our Akt (pan) (C67E7) Rabbit mAb #4691 does not detect over-expressed or endogenous levels of PKC. The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 has been tested succe... These samples represent the non-reduced protein and the denatured protein reduced by DDT. Standard western blotting protocols use denaturing conditions to expose epitopes, therefore the r... We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with human samples. These are:
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??
How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?
Have species been tested if they are not listed as cross-reactive with a CST antibody on the datasheet?
Can CUT&RUN enriched DNA be analyzed by qPCR, or do I have to do NGseq to analyze my data?
Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&RUN experiments?
Do I need to perform Fc blocking when using rabbit or mouse antibodies in flow cytometry?
How do the Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 and the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 compare in terms of their sensitivity and specificity?
Does your Akt (pan) (C67E7) Rabbit mAb #4691 detect PKC?
Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?
The gel images for your mFc-Tag products show reduced and non-reduced samples. Can you tell me what this means?
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
Why do the TCF4/TCF7L2 antibodies detect two bands?
The two dominant bands detected by our TCF4/TCF7L2 antibodies (#2565, #2569, and #2953) represent different splice variants of TCF4/TCF7L2 [see Weise, A. (2010) Nucleic Acids Res 38, 1964-81 (PMID: 20...