Technical Support Articles
Results (711)
Which total Akt antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have several monoclonal antibodies for detecting total Akt protein that are validated for IHC-P in human samples. These are as follows:
- Akt (pan) (C67E7) Rabbit mAb #46...
Which total Akt antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several monoclonal antibodies for detecting total Akt protein that are validated for IHC-P with mouse samples. These are as follows:
- Akt (pan) (C67E7) Rabbit mAb #...
What advice do you have for collecting samples of adherent cell lines versus suspension cell lines for CUT&Tag experiments?
For CUT&Tag assays using adherent cell lines, the first step is to detach the cells from the dish. We recommend using trypsin to detach the cells. Alternatively, Accutase Cell ...
Which Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several antibodies for detecting p44 and p42 MAPK when phosphorylated at Thr202 and Tyr204 of Erk1, or Thr185 and Tyr187 of Erk2,<...
Does the PathScan® Total Androgen Receptor Sandwich ELISA Kit #12850 recognize the AR-V7 isoform?
Our PathScan® Total Androgen Receptor Sandwich ELISA Kit #12850 recognizes full-length AR protein and is predicted to also recognize the AR-V7 isoform.
How do I determine the concentration of my fractionated samples using the Cell Fractionation Kit #9038?
Since the buffers in the Cell Fractionation Kit #9038 contain SDS, you should use a commercially available ready-to-use, detergent- and reducing agent-compatible assay to quickly m...
Why is there a difference in the amino acid region of Human ACE2 (18-652) Recombinant Protein (mFc-Tag) #83986 and Human ACE2 (18-615) Recombinant Protein (8xHis-Tag) #73775?
The slight difference in the amino acid span of these products is not significant for binding to SARS-CoV-2. The Human ACE2 (18-652) Recombinant Protein (mFc-Tag) #839...
What isoforms does the TGF-β Antibody #3711 detect and will it detect the 25kDa mature form of TGF-β?
The TGF-β Antibody #3711 detects recombinant TGF-β1, TGF-β2, and TGF-β3. The antibody also detects endogenous levels of the TGF-β1 precursor proteins. The different sizes we have l...
Will your GFP antibodies detect Turbo GFP?
The GFP Antibody #2555, GFP (4B10) Mouse mAb #2955, GFP (5G4) Mouse mAb #55494, and GFP (D5.1) XP® Rabbit mAb #2956 are not expected to detect Turbo GFP due to low sequence ...
Why do your Glucocorticoid Receptor (D6H2L) XP® #12041 and Glucocorticoid Receptor (D8H2) XP® #3660 Rabbit mAbs detect two bands at 91 and 94 kDa?
From our testing, we have seen that our Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb #12041 detects both the alpha and beta isoforms of the Glucocorticoid Receptor. It is possibl...
Can I purchase the components of the Senescence β-Galactosidase Staining Kit #9860 as stand alone products?
Unfortunately, due to the nature of lot production, none of the components within the Senescence β-Galactosidase Staining Kit #9860 are available for individual purchase outside of...
Do your PD-1 and PD-L1 antibodies recognize the intracellular or extracellular domain?
The regions recognized by our PD-1 and PD-L1 antibodies are as follows:
PD-1 (EH33) Mouse mAb (IHC-Specific) #43248 binds the extracellular domain.
PD-1 (D7D5W) XP® Rabbi...
Why is it important to include both positive and negative controls in your IF experiment?
Positive and negative controls are at the heart of any good experiment. Without them, it is difficult to assess root causes when troubleshooting. Positive controls should be includ...
Has your Sox2 (D6D9) XP® Rabbit mAb #3579 been tested for cross-reactivity with other SOX family members?
We have not tested our Sox2 (D6D9) XP® Rabbit mAb #3579 for cross-reactivity with other SOX family transcription factors. However, sequence alignments have been done between the an...
Do the recombinant SARS-CoV-2 spike proteins from CST have the same folding as the native SARS-CoV-2 spike protein?
Unfortunately, we cannot confirm that the recombinant SARS-CoV-2 spike proteins we provide are in exactly the same conformation that would be expected in nature, as we do not yet h...
How do you make the 4X RNA Denaturing Buffer for RNA dot blot?
4X RNA Denaturing Buffer Recipe for use with our RNA dot blot protocol:
657ul Formamide @99.5%
210ul of 37% Formaldehyde
133ul of 10x MOPS buffer
Final formulat...
What is the species cross-reactivity of your Anti-rabbit IgG, HRP-linked Antibody #7074?
It has been determined that our Anti-rabbit IgG, HRP-linked Antibody #7074 has the following low cross-reactivities with species other than rabbit:
- Guinea Pig IgG, Mouse Ig...
What is an appropriate positive control to include in this SignalStar™ assay? Are multiple controls necessary?
Any tissue shown to be positive for each marker via chromogenic IHC can serve as a positive control tissue for a SignalStar™ assay. Each target will therefore require a positive co...
My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?
Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are st...
I don’t see my target of interest in your menu of available antibodies for SignalStar™ Multiplex IHC. Can I still use it in my panel in some way?
SignalStar Multiplex IHC kits and reagents haven’t yet been validated for use with antibodies outside of our menu. We’re in the proces...
Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Mouse” in the first step of the SignalStar Multiplex IH...
Can I combine antibodies used in a SignalStar™ assay with direct conjugates?
SignalStar Multiplex IHC kits and reagents have been validated for use in combination with direct conjugates. The SignalStar™ Fluorescence Removal Kit During the course of optimization, we’ve found that fluorescent staining may show higher %-positivity than chromogenic staining. To ensure any excess staining is specific, confirm ... When selecting an antibody for use in CUT&RUN, we recommend starting with a ChIP or ChIP-seq-validated antibody; however, we have found that not all ChIP-validated antibodie... SignalStar™ Multiplex IHC Kits & Reagents have not been validated for use in combination with other assays. The SignalStar technology does not destroy the tissue, so it may be ... No, these racks cannot be autoclaved. Exposing them to high autoclave temperatures may cause the magnets to lose their magnetism. Instead, we suggest submerging or spraying the ... We have several antibodies for detecting the phosphorylation of Akt at Ser 473 that are validated for IF-IC with human samples. These include the following:
When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. How do I know if this excess staining is correct?
What is the best way to choose an antibody for my CUT&RUN assay if I cannot find a CUT&RUN validated antibody for my target protein?
Can my slides be used for other assays after SignalStar™ Multiplex IHC?
Can I autoclave the 6-Tube Magnetic Separation Rack #7017?
Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) (IF-IC) with human samples?
How long after the completion of staining can I wait to image my slides when performing a SignalStar™ Multiplex IHC assay?
For Imaging Round 1, the staining should show robust signal when imaged up to 8 hours post completion of staining. For Imaging Round 2, imaging should be performed as close to the ...
Is PTMScan® compatible with SILAC?
PTMScan® is compatible with the use of stable isotope labeling using amino acids in cell culture, or SILAC. The incorporation of heavy stable iso...
Which cleaved caspase-3 (Asp175) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with mouse samples?
We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IF-IC with mouse samples. These are:
- Cleaved Caspase-3 (Asp175) Antibody #9...