Technical Support Articles

Results (690)

Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?

Yes, please select “Human” in the first step of the SignalStar Multiplex IHC Panel Builder ...

Why do I need to use the three buffers in the Cell Fractionation Kit #9038?

Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p...

Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?

The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:

PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2B

PMID: 249...

How should I resuspend and aliquot the PTMScan® LysC Protease #84748?

To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, spin the vial at 1,0...

How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?

We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reagents into single use ...

How are the SignalStar™ Multiplex IHC Kits & Reagents validated?

CST thoroughly validates each antibody available in the

Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??

Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77...

Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?

The SignalStar Multiplex IHC kits and reagents have been optimized with respect to fluorophore pairing and order of antibodies. As tissues vary in quality and expression level of biomarkers, increasin...

How do the Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 and the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 compare in terms of their sensitivity and specificity?

The Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 is a mouse mAb from a single clone, whereas the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 is a mix of rabbit mAbs from multiple clon...

Can I reuse PTMScan® antibody beads?

The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic elution step, which l...

Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?

We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include:

β-Actin (13E5) Rabbit mAb #4970

β-Actin (D6A8) Rabbit mAb #...

Is PTMScan® compatible with SILAC?

PTMScan® is compatible with the use of stable isotope labeling using amino acids in cell culture, or SILAC. The incorporation of heavy stable isotopes has no effect ...

Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?

The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 has been tested succe...

Can I use CST antibodies for fluorescent detection in FFPE tissue?

At CST we do not typically validate antibodies using indirect fluorescent detection in formalin fixed, paraffin embedded (FFPE) tissue. If a product is recommended for use in chromogenic IHC, denoted ...

Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&RUN experiments?

The Concanavalin A beads can clump together rather easily, especially when the cells become lysed. To avoid this, make sure your cells are healthy and be sure to treat them very gently during the cell...

What DNA library prep kit do you recommend using for CUT&RUN?

We recommend using our SimpleChIP^® ChIP-seq DNA Library Prep Kit for Illumina^® #567...

In terms of cytokine activity, what is the difference between International Units (IU) and specific activity?

International Units (IU) and specific activity are two different ways of measuring...

Can the Cell Fractionation Kit #9038 be used for frozen cell pellets?

We have had customers perform this assay using frozen cell pellets, prior to adding any fractionation buffer, and the subsequent fractionation worked fine. Once you start fractionating, we recommen...

How does the specificity of the F(ab’)2 fragments differ from the corresponding full-length monoclonal antibodies?

Since the paratopes of the antibody fragments are identical to that of the corresponding full-length monoclonal antibodies, their specificity for scFv linkers remains unchanged. However, the abs...

Do I need to perform Fc blocking when using rabbit or mouse antibodies in flow cytometry?

Human Fc-gamma Receptor 1a (FCGR1A) exhibits strong cross-reactivity with rabbit IgG, and mouse IgG2a, IgG2c, and IgG3. Of course, mouse Fc-gamma Receptors will react with mouse antibodies. In flow cy...

Does this antibody work for my application (western blot, immunoprecipitation, immunofluorescence, immunohistochemistry, ELISA, flow cytometry, chromatin immunoprecipitation, etc.)?

CST routinely tests antibodies in relevant applications. Those that pass rigorous application-specific validation standards are recommended. We only recommend applications that have passed rigorous va...

Does your Cas9 (7A9-3A3) Mouse mAb #14697 detect the D10A/H840A mutant (dCas9) and the D10A mutant (niCas9)?

Our Cas9 (7A9-3A3) Mouse mAb #14697 was produced with a recombinant protein specific to the amino terminus of Cas9 from Streptococcus pyogenes (UniProt ID #Q99ZW2; https://www.uniprot.org/unipr...

Can I order a custom formulation of an antibody?

For custom (carrier-free) orders, many antibodies are available pre-formulated in PBS without BSA or glycerol. This carrier-free formulation is amenable to conjugation and can be conjugated to fluorop...

Are Smad8 and Smad9 the same protein?

Smad8 and Smad9 are often used interchangeably in the literature to refer to the same protein. However, the official name of the protein is Smad9. Moreover, there is a Smad8 in Xenopu...

Which cleaved caspase-3 (Asp175) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?

We have several antibodies for detecting Cleaved Caspase-3 (Asp175) that are validated for IF-IC with human samples. These are:

Cleaved Caspase-3 (Asp175) Antibody #9661

Cleaved Caspas...
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?

We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with human samples. These are:
- Cleaved ...
  Which S6 ribosomal protein antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) with mouse samples?
  
  We have several antibodies for detecting ribosomal protein S6 (rpS6) that are validated for IF-IC with mouse samples. These are S6 Ribosomal Protein (5G10) Rabbit mAb #2217 and S6 Ribosomal Protein (5...
  Which phospho-AMPKα antibody do you suggest for my western blot experiment?
  
  The selection of a phospho-AMPKα antibody depends on the subunit of interest. Cell Signaling Technology has four antibodies that target phospho-AMPKα:
  - Phospho-AMPKα (Thr172) (40H9) R...
    Does the CUT&Tag assay show a bias toward euchromatin or heterochromatin?
    
    With the appropriate salt concentration in the tagmention buffer, we don't observe bias toward euchromatin or heterochromatin in our assays. In CUT&Tag, the target-specific antibody and the se...
    Does CUT&RUN show a bias toward euchromatin or heterochromatin?
    
    We do not observe any bias toward euchromatin or heterochromatin in our assays. In CUT&RUN, the target-specific antibody recruits the pAG-MNase and directs digestion to chromatin that is directly ...