Technical Support Articles

What is the expected signal strength for the PTMScan® Control peptides?

We have observed >1E6 peak area for each control peptide when used at the supplied concentration followed by PTMScan and PTMScan HS enrichment, as detected by our...

What is the benefit of carrier vs. carrier free cytokines?

Our cytokines and growth factors are lyophilized and supplied either with 20ug BSA per 1ug of cytokine (with carrier) or without added protein (carrier free). BSA minimizes adsorption to surfaces and ...

Can the Lambda Protein Phosphatase Kit #89726 be stored at -20°C?

The Lambda Protein Phosphatase #56206 included in the Lambda Protein Phosphatase Kit #89726 is temperature sensitive. Therefore, we recommend storing the kit at -80°C. However, the kit is stable at -2...

Is there a maximum number of cells that can be used in a single CUT&Tag assay?

We have found 100,000 cells per assay to be sufficient for enriching target loci for multiple histone modifications, transcription factors, and transcription cofactors. However, one can increase the n...

Which phospho-S6 ribosomal protein (Ser235/236) antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) with mouse samples?

We have several antibodies for detecting the phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 that are validated for IF-IC with mouse samples. These are Phospho-S6 Ribosomal Protein (Ser23...

How do I avoid batch effects and what sample run order should I use for PTMScan® experiments?

To avoid intra-sample batch effects during liquid chromatography with tandem mass spectrometry (LC-MS/MS), we recommend a run order that involves running one technical replicate of each enrichment, th...

Why isn't my bottle of Cell Lysis Buffer (10X) #9803 frozen?

Occasionally, Cell Lysis Buffer (10X) #9803 stored at -20C may not appear frozen, which is not a reason for concern. This phenomenon is known as nucleation, or the initial formation of ice from a liqu...

What CD45 isoforms are detected by the CD45 (D9M8I) XP (#13917), CD45 (D3F8Q) (#70257), and CD45 (D4H7K) ( #72787) Rabbit mAbs?

If CST recommended buffers are being used for western blotting and high background or additional bands persist when probing for phospho-AMPK (Thr172), the issue could be related to the lysate sample. ...

Can the Phospho-β-Catenin (Ser33/37) Antibody #2009 bind if the sites are singly phosphorylated or is the phosphorylation at both sites necessary?

We have tested the reactivity of the Phospho-β-Catenin (Ser33/37) Antibody #2009 to Ser33/37 by peptide dot blot. This antibody showed strong signal when phosphorylated at Ser37 alone and when phospho...

Will your GFP antibodies detect Turbo GFP?

The GFP Antibody #2555, GFP (4B10) Mouse mAb #2955, GFP (5G4) Mouse mAb #55494, and GFP (D5.1) XP® Rabbit mAb #2956 are not expected to detect Turbo GFP due to low sequence homology.

Why does the signal for LC3-II increase with chloroquine, an inhibitor of autophagy?

The abundance of LC3-II is generally very low without treatment. Glucose starvation, amino acid starvation, serum starvation, and rapamycin treatment have been shown in the literature to induce increa...