Technical Support Articles
Results (694)
Can I store the resuspended PTMScan® LysC Protease #84748 aliquots at -20°C instead of -80°C?
Although the lyophilized powder can be stored at -20°C, we recommend long-term storage of the resuspended LysC at -80°C. We do not recommend long-term storage of the resuspended LysC at -20°C due to p...
Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?
We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include:
- β-Actin (13E5) Rabbit mAb #4970
- β-Actin (D6A8) Rabbit mAb #...
How are the SignalStar™ Multiplex IHC Kits & Reagents validated?
CST thoroughly validates each antibody available in the The Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 is a mouse mAb from a single clone, whereas the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 is a mix of rabbit mAbs from multiple clon... The most sensitive antibody will depend on whether you are trying to detect Cas9 protein from Streptococcus pyogenes, Staphylococcus aureus, or Campylobacter jejuni. The Cas9 (7A9... We currently have four different secondary antibodies approved for use in fluorescent western blotting, depending on species and wavelength of detection:
Our cytokines and growth factors are lyophilized and supplied either with 20ug BSA per 1ug of cytokine (with carrier) or without added protein (carrier free). BSA minimizes adsorption to surfaces and ... Yes, please select “Mouse” in the first step of the SignalStar Multiplex IHC Panel Builder ... Whether performing CUT&RUN with downstream qPCR or NG-seq analysis, we always recommend using a CUT&RUN-validated positive control antibody like our Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex... Although LysC can digest proteins at high urea concentrations (~8M), we recommend diluted urea (~2M) to minimize the risk of carbamylation. When selecting an antibody for use in CUT&RUN, we recommend starting with a ChIP or ChIP-seq-validated antibody; however, we have found that not all ChIP-validated antibodies work in the CUT&am... As long as your Concanavalin A Magnetic Beads #82307 have been stored at the appropriate recommended temperature, the appearance of aggregation is not a cause for concern. By nature, the bead particle... Our β-Actin (13E5) Rabbit mAb #4970 detects endogenous levels of total β-actin protein. Despite the high sequence identity between the cytoplasmic actin isoforms, β-actin and cytoplasmic γ-actin, our ... CST does not develop custom antibodies. However, we are happy to accept your target suggestions. Please We have observed >1E6 peak area for each control peptide when used at the supplied concentration followed by PTMScan and PTMScan HS enrichment, as detected by our... Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p... Echinomycin #51434 is lyophilized into the vial, and any difference in the appearance of the lyophilized echinomycin is due to a dry coating of the compound in the vial. When you add dimethylsulfoxide... To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, spin the vial at 1,0... We have shown that our CUT&Tag Assay Kit #77552 works with as few as 5,000-10,000 cells for histone modifications and 20,000 cells for transcription factors and cofactors. The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers: We have several monoclonal antibodies for studying p44 and p42 mitogen-activated protein kinases (MAPKs), also referred to as Erk2 and Erk1, that are validated in IF-IC with mouse tissues. These are p... Yes, please select “Human” in the first step of the SignalStar Multiplex IHC Panel Builder ... SignalStar Multiplex IHC kits and reagents have been validated for use in combination with direct conjugates. The SignalStar™ Fluorescence Removal Kit The SignalStar Multiplex IHC kits and reagents have been optimized with respect to fluorophore pairing and order of antibodies. As tissues vary in quality and expression level of biomarkers, increasin... When performing In-Cell Western assays with DyLight 680-conjugated secondary antibodies, we recommend using either Hoechst 33342 or DAPI as a nuclear dye. DRAQ5 cannot be used because the emission spe... Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77... SignalStar™ Multiplex IHC Kits & Reagents have not been validated for use in combination with other assays. The SignalStar technology does not destroy the tissue, so it may be possible to perform ... We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reagents into single use ...How do the Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 and the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 compare in terms of their sensitivity and specificity?
How does the specificity of the F(ab’)2 fragments differ from the corresponding full-length monoclonal antibodies?
Which Cas9 antibody is the most sensitive by western immunoblot?
What secondary antibodies do you recommend for fluorescent western blotting?
What is the benefit of carrier vs. carrier free cytokines?
Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?
What controls can I use to show my CUT&RUN experiment is working?
My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?
Why is the PTMScan® LysC Protease #84748 digestion performed in a diluted urea formulation?
What is the best way to choose an antibody for my CUT&RUN assay if I cannot find a CUT&RUN validated antibody for my target protein?
Why do my Concanavalin A Magnetic Beads #82307 appear aggregated?
What isoforms are detected by your β-Actin (13E5) Rabbit mAb #4970?
Can CST develop an antibody for me?
What is the expected signal strength for the PTMScan® Control peptides?
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Why does the material in my new vial of Echinomycin #51434 look different?
How should I resuspend and aliquot the PTMScan® LysC Protease #84748?
What is the fewest number of cells I can use in a reaction with the CST CUT&Tag kit?
Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?
PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2B
PMID: 249...Which p44/42 MAPK (Erk1/2) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with mouse samples?
Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?
Can I combine antibodies used in a SignalStar™ assay with direct conjugates?
Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?
Which nuclear dyes can be used for In-Cell Western assays in combination with DyLight 680-conjugated secondary antibodies?
Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??
Can my slides be used for other assays after SignalStar™ Multiplex IHC?
How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?