Technical Support Articles

Results (689)

Can I modify the CST-recommended protocol for a given application?

CST antibodies undergo thorough validation processes to ensure reliable performance for every approved application. This includes testing different protocol variations to identify the most effective o...

Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?

Yes, please select “Human” in the first step of the SignalStar Multiplex IHC Panel Builder ...

Why do I need to use the three buffers in the Cell Fractionation Kit #9038?

Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p...

How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?

We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reagents into single use ...

How should I resuspend and aliquot the PTMScan® LysC Protease #84748?

To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, spin the vial at 1,0...

Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?

The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:

PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2B

PMID: 249...

Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??

Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77...

Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?

The SignalStar Multiplex IHC kits and reagents have been optimized with respect to fluorophore pairing and order of antibodies. As tissues vary in quality and expression level of biomarkers, increasin...

How are the SignalStar™ Multiplex IHC Kits & Reagents validated?

CST thoroughly validates each antibody available in the

Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?

The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 has been tested succe...

Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?

We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include:

β-Actin (13E5) Rabbit mAb #4970

β-Actin (D6A8) Rabbit mAb #...

Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&RUN experiments?

The Concanavalin A beads can clump together rather easily, especially when the cells become lysed. To avoid this, make sure your cells are healthy and be sure to treat them very gently during the cell...

How does the specificity of the F(ab’)2 fragments differ from the corresponding full-length monoclonal antibodies?

Since the paratopes of the antibody fragments are identical to that of the corresponding full-length monoclonal antibodies, their specificity for scFv linkers remains unchanged. However, the abs...

Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?

Yes, please select “Mouse” in the first step of the SignalStar Multiplex IHC Panel Builder ...

Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?

We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with human samples. These are:

Cleaved Caspase-3 (Asp175) Antibody #9661

Cleaved ...

My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?

Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex...

In terms of cytokine activity, what is the difference between International Units (IU) and specific activity?

International Units (IU) and specific activity are two different ways of measuring...

What is the best way to choose an antibody for my CUT&RUN assay if I cannot find a CUT&RUN validated antibody for my target protein?

When selecting an antibody for use in CUT&RUN, we recommend starting with a ChIP or ChIP-seq-validated antibody; however, we have found that not all ChIP-validated antibodies work in the CUT&am...

Can I order a custom formulation of an antibody?

For custom (carrier-free) orders, many antibodies are available pre-formulated in PBS without BSA or glycerol. This carrier-free formulation is amenable to conjugation and can be conjugated to fluorop...

Why is the concentration of my antibody so low?

At CST, our antibodies are formulated on the basis of their reactivity and performance for each approved application at a recommended dilution. For antibodies with high affinity, optimal performan...

I don’t see my target of interest in your menu of available antibodies for SignalStar™ Multiplex IHC. Can I still use it in my panel in some way?

SignalStar Multiplex IHC kits and reagents haven’t yet been validated for use with antibodies outside of our menu. We’re in the process of developing cust...

Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?

We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with mouse samples. These are: