Technical Support Articles
Results (709)
Which cleaved caspase-3 (Asp175) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
We have several antibodies for detecting Cleaved Caspase-3 (Asp175) that are validated for IF-IC with human samples. These are:
- Cleaved Caspase-3 (Asp175) Antibody #9661
Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&RUN experiments?
The Concanavalin A beads can clump together rather easily, especially when the cells become lysed. To avoid this, make sure your cells are healthy and be sure to treat them very ge...
Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?
The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 h...
How does the specificity of the F(ab’)2 fragments differ from the corresponding full-length monoclonal antibodies?
What is the solution formulation of the PTMScan Control peptides?
The solution formulation of the PTMScan Control peptides is 25% acetonitrile, 74.9% water, 0.1% trifluoroacetic acid (V:V).<...
Does the Human IL-22 Recombinant Protein #74808 contain any human or animal material and is it free of HIV/AIDS, HCV/HBC or other infectious material?
No materials of animal origin are used in the production of our Human IL-22 Recombinant Protein #74808. Therefore, additional testing for infectious material is not relevant.
How much of each antibody is supplied in the StemLight™ Pluripotency Antibody Kit #9656 and how does this relate to the number of tests?
There is 50 µl of each antibody component that is listed in the StemLight Pluripotency Antibody Kit #9656. The kit components are pre-optimized for parallel use in immunofluorescen...
Does your Cas9 (7A9-3A3) Mouse mAb #14697 detect the D10A/H840A mutant (dCas9) and the D10A mutant (niCas9)?
Our Cas9 (7A9-3A3) Mouse mAb #14697 was produced with a recombinant protein specific to the amino terminus of Cas9 from Streptococcus pyogenes (UniProt ID #Q99ZW2; https://w...
How should I search for the Pan-Methyl Lysine PTM in LC-MS/MS data when using your PTMScan® Pan-Methyl Lysine Kit #14809?
Our PTMScan Pan-Methyl Lysine Kit #14809 will enrich for all three forms of methylated lysine: mono, di and tri-methyl lysine. To reduce the complexity of the search space, it is n...
Which species does the Caspase-3 Activity Assay Kit #5723 work in?
We have validated the Caspase-3 Activity Assay Kit #5723 in human and mouse cell lines. However, as long as there is active caspase-3 present, this kit is predicted to work with mo...
Can I use a BCA assay to check the concentration of my antibody?
A BCA protein assay will likely not provide an accurate concentration for our antibodies formulated in glycerol, due to the presence of BSA and incompatibility with a higher percen...
Does your Ki-67 (D2H10) Rabbit mAb #9027 cross-react with mouse tissue by IHC?
We have tested the Ki-67 (D2H10) Rabbit mAb #9027 on a variety of FFPE mouse tissues and have not observed specific staining. Based on this, we have concluded that #9027 does not d...
Can your Protease/Phosphatase Inhibitor Cocktail (100X) #5872 be used for mass spectrometry?
Yes, our Protease/Phosphatase Inhibitor Cocktail (100X) #5872 can be used in mass spectrometry (MS) experiments. This cocktail does not contain AEBSF, which can cause peaks to shif...
Can you share your protocol for preparing Jurkat -/+ Anti-CD3 antibody (10 µg/mL for 2 min) cell extracts?
We prepare Jurkat -/+ Anti-CD3 antibody (10 µg/mL for 2 min) cell extracts as follows:
- Jurkat cells are cultured to the desired volume (200-400 mL) and a density o...
Which desalting columns do you recommend to use with the Malachite Green Phosphate Detection Kit #12776 in order to remove contaminating phosphate from the cells?
When developing the Malachite Green Phosphate Detection Kit #12776, we successfully used the Millipore Centrifuge filters AMICON ULTRA 0.5mL, 10,000 NMWL, product # UFC501096.
Why does MAFA (D2Z6N) Rabbit mAb #79737 recognize a band at approximately 48 kDa when the predicted molecular weight is approximately 37 kDa?
MAFA is a phosphoprotein. The multiple phosphorylation sites on this protein cause a mobility shift, resulting in the protein migrating slower by gel electrophoresis. This has been...
Does your Tau (D1M9X) XP® Rabbit mAb #46687 recognize all six Tau isoforms?
Our Tau (D1M9X) XP® Rabbit mAb #46687 is predicted to recognize 2N4R (tau40), 2N3R (tau39), 1N4R (tau46), 1N3R (tau37), 0N4R (tau24), and 0N3R (tau23), as all six isoforms include ...
Will your Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625 also recognize the smaller PARP-1 fragments (40-55 kDa) that are generated during necrosis?
Our Cleaved PARP (Asp214) (D64E10) XP Rabbit mAb #5625 is designed to be specific for the large fragment (89 kDa) of human PARP1 protein that is produced by caspase cleavage at Asp...
Can my CST antibody be used for fluorescent western blot?
We do not routinely validate our products for use in fluorescent western blot; however, many are expected to work well in this application with a few protocol modifications. When p...
What is the expected CUT&RUN DNA yield when starting with extremely low cell numbers, such as 5,000 - 10,000 cells?
If you start with 100,000 cells, you typically get 0.6-6ng DNA. However, if you start with 5,000 - 10,000 cells, the DNA yield can be as low as 0.1-0.2 ng or even undetectable by t...
Which phospho-S6 ribosomal protein (Ser235/236) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have several antibodies for detecting the phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 that are validated for IHC-P with human samples. These are Phospho-S6 Ribo...
Can your Streptavidin-HRP #3999 be used for ELISA?
Regrettably, we do not test our Streptavidin-HRP #3999 for use in ELISA . Therefore, we cannot provide any firm guarantees or a recommended dilution. However, it should work for EL...
How do I avoid batch effects and what sample run order should I use for PTMScan® experiments?
To avoid intra-sample batch effects during liquid chromatography with tandem mass spectrometry (LC-MS/MS), we recommend a run order that involves running one technical replicate of...
Why do I see a doublet when using my total Histone H3 antibody for western blot?
We see this doublet phenomenon often in a number of different cell lines that we test with our Histone H3 antibodies. It does appear to be sample dependent. The doublet may be due ...
Can I purchase Solution B outside of the Senescence β-Galactosidase Staining Kit #9860?
We are currently unable to provide the Solution B component separately from the Senescence β-Galactosidase Staining Kit #9860. However, we can disclose the ingredients of Solution ...
Why am I seeing dark background all over my western blot when using your Phospho-p53 (Ser15) Antibody #9284?
It is critical that our Phospho-p53 (Ser15) Antibody #9284 be diluted into 5% w/v non-fat dry milk, 1X TBS, 0.1% Tween® 20 and incubated at 4°C with gentle shaking, over...
Why does the PINK1 (D8G3) Rabbit mAb #6946 detect two bands for human PINK1?
In endogenous samples, the PINK1 precursor protein (~60kDa), is imported into mitochondria where it is processed into a cleaved form (~50kDa) that is transported back to the cytopl...
Why are there two non-reduced bands in the gel image for your Human ACE2 (18-652) Recombinant Protein (mFc-Tag) #83986?
The top band in the gel image for our Human ACE2 (18-652) Recombinant Protein (mFc-Tag) #83986 likely represents dimerization due to the mFc tag.
What is the difference between the discontinued anti-rabbit and anti-mouse PE secondary antibodies #8885 & #8887 and their replacements, #79408 & #59997
We have decided to no longer support the anti-rabbit and anti-mouse PE secondary antibodies, #8885 and #8887. We have developed two new secondaries which prove to be superior to th...
Why is there an additional band at 24kDa using the Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718?
The extra band(s) around 24 kDa using our Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718 are most likely mono-ubiquitylated H2AX. H2AX, like H2A, is mono-ubiquitylated.