Technical Support Articles
Results (690)
Is it possible to perform CUT&Tag on samples like heart, brain, or developing mouse tissues?
Yes. We have shown that our CUT&Tag Assay Kit #77552 works with mouse liver, mouse brain, and mouse heart tissues for histone targets with as little as 1 mg of tissue for each CUT&Tag reaction...
Do you have a recommended protocol for stripping/re-probing blots?
The western blot stripping/re-probing protocol for our Western Blot Stripping Buffer (5X) #91925 is below. However, it is important to note that we typically advise against stripping/re-probing becaus...
Have you done any cross-reactivity testing with α-CGRP, β-CGRP and unprocessed precursors using the CGRP (D5R8F) Rabbit mAb #14959?
Our CGRP (D5R8F) Rabbit mAb #14959 is produced with a synthetic peptide corresponding to residues surrounding Val114 of human CGRP protein (UniProt ID P06881 isoform 3; http://www.uniprot.org/uniprot/...
Can I use your Protein A Agarose Beads #9863 and Protein G Agarose Beads #37478 if they have been frozen?
Although we are unable to guarantee that both our Protein A Agarose Beads #9863 and Protein G Agarose Beads #37478 will work after being stored at -20C, we have used beads that have been frozen and th...
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p...
Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?
The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:
PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2B
PMID: 249...
Can I order a custom formulation of an antibody?
For custom (carrier-free) orders, many antibodies are available pre-formulated in PBS without BSA or glycerol. This carrier-free formulation is amenable to conjugation and can be conjugated to fluorop...
How do the Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 and the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 compare in terms of their sensitivity and specificity?
The Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 is a mouse mAb from a single clone, whereas the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 is a mix of rabbit mAbs from multiple clon...
What controls should I include when performing a Simple Western™ experiment?
In addition to a known positive control sample, we recommend the following negative controls for your Simple Western™ experiment.
1. A no lysate control. This includes the primary antibody at t...
Why can't I add a target to the channel I want when using the SignalStar™ Multiplex IHC Panel Builder?
During our extensive validation process, we found that some targets do not perform optimally with specific fluorophore pairings. When this is the case, the SignalStar Multiplex IHC Panel Builder will ...
Which total Akt antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
We have several antibodies for detecting total Akt protein that are validated for IF-IC with human samples. These are as follows:
- Akt (pan) (C67E7) Rabbit mAb #4691
- Akt (pan) (11E7) Ra...
Why do my Concanavalin A Magnetic Beads #82307 appear aggregated?
As long as your Concanavalin A Magnetic Beads #82307 have been stored at the appropriate recommended temperature, the appearance of aggregation is not a cause for concern. By nature, the bead particle...
What is the shelf life of my product?
Information about the shelf life of our products can be found at the following link: We recommend washing the Protein A and Protein G magnetic beads prior to use to help clear them of any potential increase in background due to the buffer in which they are provided. CST does not develop custom antibodies. However, we are happy to accept your target suggestions. Please The Hematoxylin #14166 formula is based on Gill's formulation however, this formulation does not contain alcohol or mercury. We currently have four different secondary antibodies approved for use in fluorescent western blotting, depending on species and wavelength of detection:
The molecular weight reported by Simple Wesetern™ instruments like Jess™ is an "apparent" molecular weight as opposed to an "exact" molecular weight. The molecular weight of the ta... Concanavalin A beads can clump together rather easily, especially when cells become lysed. Make sure your cells are healthy and be sure to treat them very gently during the cell wash. We also recommen... We have several antibodies for detecting the phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 that are validated for IF-IC with mouse samples. These are Phospho-S6 Ribosomal Protein (Ser23... For PTMScan experiments, the stage tip, or small-scale peptide desalting is a critical part of the experiment for a number of reasons. Detailed instructions for performing stage tip peptide desalting ... Due to the expected and typically low yield of CUT&Tag DNA, we recommend using all 30 µL of the CUT&Tag DNA sample (from The extra band(s) around 24 kDa using our Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718 are most likely mono-ubiquitylated H2AX. H2AX, like H2A, is mono-ubiquitylated. The PTMScan® technology is compatible with samples derived from all kingdoms of life as well as samples containing mixed species like xenografts (mouse and human), microbiome samples (bacterial and ho... We have not tested our Sox2 (D6D9) XP® Rabbit mAb #3579 for cross-reactivity with other SOX family transcription factors. However, sequence alignments have been done between the antigen used to produc... Occasionally, Cell Lysis Buffer (10X) #9803 stored at -20C may not appear frozen, which is not a reason for concern. This phenomenon is known as nucleation, or the initial formation of ice from a liqu... Our β-Actin (13E5) Rabbit mAb #4970 detects endogenous levels of total β-actin protein. Despite the high sequence identity between the cytoplasmic actin isoforms, β-actin and cytoplasmic γ-actin, our ... We have several antibodies for ribosomal protein S6 (rpS6) that are validated for IHC-P with mouse samples. These are S6 Ribosomal Protein (5G10) Rabbit mAb #2217 and S6 Ribosomal Protein (54D2) Mouse... Specific activity (expressed in units/mg) is a function of cytokine or growth factor purity and appropriate protein folding. To determine the specific activity, typically the readout of cytokine treat... The concentration of the pAG-MNase Enzyme #57813 in our CUT&RUN kits is 25ug/ml.Is prewashing the Protein A Magnetic Beads #73778 and Protein G Magnetic Beads #70024 necessary for my IP experiment?
Can CST develop an antibody for me?
What is the formulation of Hematoxylin #14166?
What secondary antibodies do you recommend for fluorescent western blotting?
Why is there a discrepancy in the molecular weight of my target when analyzed by traditional western blot and Simple Western™?
Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&Tag experiments?
Which phospho-S6 ribosomal protein (Ser235/236) antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) with mouse samples?
Why should I include a stage tip step in my PTMScan® experiment?
What if my yield of CUT&Tag DNA is too low to see on an Agilent Bioanalyzer or TapeStation system?
Why is there an additional band at 24kDa using the Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718?
Can I use mixed species samples for a PTMScan® experiment?
Has your Sox2 (D6D9) XP® Rabbit mAb #3579 been tested for cross-reactivity with other SOX family members?
Why isn't my bottle of Cell Lysis Buffer (10X) #9803 frozen?
What isoforms are detected by your β-Actin (13E5) Rabbit mAb #4970?
Which ribosomal protein S6 (rpS6) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
Can you help me calculate the bioactivity of my cytokine?
What is the concentration of the pAG-MNase Enzyme #57813?