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Why do I need to use the three buffers in the Cell Fractionation Kit #9038?

Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p...

How should I resuspend and aliquot the PTMScan® LysC Protease #84748?

To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, spin the vial at 1,0...

Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?

Yes, please select “Human” in the first step of the SignalStar Multiplex IHC Panel Builder ...

Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?

The SignalStar Multiplex IHC kits and reagents have been optimized with respect to fluorophore pairing and order of antibodies. As tissues vary in quality and expression level of biomarkers, increasin...

Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?

The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:

PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2B

PMID: 249...

How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?

We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reagents into single use ...

Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??

Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77...

Can I modify the CST-recommended protocol for a given application?

CST antibodies undergo thorough validation processes to ensure reliable performance for every approved application. This includes testing different protocol variations to identify the most effective o...

How are the SignalStar™ Multiplex IHC Kits & Reagents validated?

CST thoroughly validates each antibody available in the

What controls can I use to show my CUT&RUN experiment is working?

Whether performing CUT&RUN with downstream qPCR or NG-seq analysis, we always recommend using a CUT&RUN-validated positive control antibody like our 

Which isoforms does the ba1/AIF-1 (E4O4W) XP® Rabbit mAb #17198 detect?

Product Iba1/AIF-1 (E4O4W) XP® Rabbit mAb #17198 is predicted to detect isoform 1 and isoform 2 of the Ionized calcium-binding adaptor molecule 1 (Iba1), also known as allograft inflammator...

Why is a fluorescent conjugate of a rabbit antibody giving no signal on compensation beads in flow cytometry?

If you are using compensation beads in your flow cytometry experiment and observe no signal with one of our fluorescent conjugates of a rabbit antibody, this may be due to the reactivity of the compen...

Why do my Concanavalin A Magnetic Beads #82307 appear aggregated?

As long as your Concanavalin A Magnetic Beads #82307 have been stored at the appropriate recommended temperature, the appearance of aggregation is not a cause for concern. By nature, the bead particle...

Should digitonin be included in my wash buffers for my entire CUT&Tag experiment?

Yes, digitonin needs to be present in all wash buffers during the entire CUT&Tag experiment. This is because cell membrane permeabilization caused by digitonin is reversible. Removing or lowering ...

Why is the PTMScan® LysC Protease #84748 digestion performed in a diluted urea formulation?

Although LysC can digest proteins at high urea concentrations (~8M), we recommend diluted urea (~2M) to minimize the risk of carbamylation.

Can CST develop an antibody for me?

CST does not develop custom antibodies. However, we are happy to accept your target suggestions. Please

What positive and negative controls can I use to demonstrate that my CUT&Tag experiment is successful?

We recommend using a CUT&Tag-validated positive control antibody, like our Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751, in your experiment to confirm that your CUT&Tag assay is worki...

What is the shelf life of my product?

Information about the shelf life of our products can be found at the following link:

Do you have a protocol for lysing in a 96-well plate (no sonication)?

For lysis in 96-well assay plates, we recommend incubation in the provided lysis buffer for ~30 min at 4°C with gentle shaking. While sonication is difficult in small volumes, we strongly recommend...

Which S6 ribosomal protein antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?

We have several monoclonal antibodies for detecting ribosomal protein S6 that are validated for IF-IC with human samples. These are S6 Ribosomal Protein (5G10) Rabbit mAb #2217 and S6 Ribosomal Pro...

What is the expected yield of a CUT&Tag DNA library?

The expected yield of an amplified CUT&Tag DNA library depends on the DNA quantification system used.