Technical Support Articles

Which species does the Caspase-3 Activity Assay Kit #5723 work in?

We have validated the Caspase-3 Activity Assay Kit #5723 in human and mouse cell lines. However, as long as there is active caspase-3 present, this kit is predicted to work with mo...

Are the light chains of the β-Actin (8H10D10) Mouse mAb #3700 kappa or lambda?

The β-Actin (8H10D10) Mouse mAb #3700 has kappa light chains.

Does your Ki-67 (D2H10) Rabbit mAb #9027 cross-react with mouse tissue by IHC?

We have tested the Ki-67 (D2H10) Rabbit mAb #9027 on a variety of FFPE mouse tissues and have not observed specific staining. Based on this, we have concluded that #9027 does not d...

What CD45 isoforms are detected by the CD45 (D9M8I) XP (#13917), CD45 (D3F8Q) (#70257), and CD45 (D4H7K) ( #72787) Rabbit mAbs?

MAFA is a phosphoprotein. The multiple phosphorylation sites on this protein cause a mobility shift, resulting in the protein migrating slower by gel electrophoresis. This has been...

Why do I see a doublet when using my total Histone H3 antibody for western blot?

We see this doublet phenomenon often in a number of different cell lines that we test with our Histone H3 antibodies. It does appear to be sample dependent. The doublet may be due ...

What is the expected CUT&RUN DNA yield when starting with extremely low cell numbers, such as 5,000 - 10,000 cells?

If you start with 100,000 cells, you typically get 0.6-6ng DNA. However, if you start with 5,000 - 10,000 cells, the DNA yield can be as low as 0.1-0.2 ng or even undetectable by t...

Which p44/42 MAPK (Erk1/2) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with mouse samples?

We have several monoclonal antibodies for studying p44 and p42 mitogen-activated protein kinases (MAPKs), also referred to as Erk2 and Erk1, that are validated in IF-IC with mouse ...

What is the concentration of the pAG-MNase Enzyme #57813?

The concentration of the pAG-MNase Enzyme #57813 in our CUT&RUN kits is 25ug/ml.

Can I use mixed species samples for a PTMScan® experiment?

The PTMScan® technology is compatible with samples derived from all kingdoms of life as well as samples containing mixed species like xenografts (mouse and human), microbiome sampl...

Are Smad8 and Smad9 the same protein?

Smad8 and Smad9 are often used interchangeably in the literature to refer to the same protein. However, the official name of the protein is Smad9. Moreover, there ...

Why isn't my bottle of Cell Lysis Buffer (10X) #9803 frozen?

Occasionally, Cell Lysis Buffer (10X) #9803 stored at -20C may not appear frozen, which is not a reason for concern. This phenomenon is known as nucleation, or the initial formatio...

Which phospho-S6 ribosomal protein (Ser235/236) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?

We have several antibodies for detecting the phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 that are validated for IHC-P with human samples. These are Phospho-S6 Ribo...

Which ribosomal protein S6 (rpS6) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?

We have several antibodies for ribosomal protein S6 (rpS6) that are validated for IHC-P with mouse samples. These are S6 Ribosomal Protein (5G10) Rabbit mAb #2217 and S6 Ribosomal ...

Where can I find the recommended primary antibody dilution buffer for western blotting (BSA or milk)?

The recommended primary antibody dilution buffer for western blotting (i.e., BSA or nonfat dry milk) can be found either in a grey box on the bottom of the first page of the produc...

Is it normal to see blue staining in my untreated samples when using the Senescence β-Galactosidase Staining Kit #9860?

Seeing positive blue staining in your untreated cells is not a cause for concern. We have also observed positive blue staining in our negative controls in some cell types. For exam...