Technical Support Articles
Results (695)
Does this antibody work for my application (western blot, immunoprecipitation, immunofluorescence, immunohistochemistry, ELISA, flow cytometry, chromatin immunoprecipitation, etc.)?
CST routinely tests antibodies in relevant applications. Those that pass rigorous application-specific validation standards are recommended. We only recommend applications that have passed rigorous va...
Why doesn't PD-L2 migrate at 31 kDa?
The predicted molecular weight of PD-L2 is 31 kDa (based on amino acid composition). However, in most models, PD-L2 migrates at an observed molecular weight of 45-60 kDa on Tris-Glycine gels. This dis...
What is the concentration and pH of your Glycine Solution (10X) #7005?
The concentration of our Glycine Solution (10X) #7005 is 1.25M and the pH is 6.5.
Why is a doublet observed by western blot with your ZEB2 antibody #97885?
ZEB2 mRNA is alternatively spliced and there are post-translational modifications (PTMs), such as ubiquitination and sumoylation, that may be the cause of the larger, 210kDa band. The potential modifi...
What is the identity and concentration of the positive control in my FastScan™ ELISA kit?
Unfortunately, the identity and concentration of the positive controls supplied in our FastScan™ kits are considered proprietary. We offer a positive control to confirm that the assay is working as ex...
Can I purchase Solution B outside of the Senescence β-Galactosidase Staining Kit #9860?
We are currently unable to provide the Solution B component separately from the Senescence β-Galactosidase Staining Kit #9860. However, we can disclose the ingredients of Solution B that is used in th...
How are the SignalStar™ Multiplex IHC Kits & Reagents validated?
CST thoroughly validates each antibody available in the Positive and negative controls are at the heart of any good experiment. Without them, it is difficult to assess root causes when troubleshooting. Positive controls should be included to demonstrate:
<... The concentration of the antibody-beads included in the PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Kit #5562 (Bead SKU #1990) is proprietary and therefore we are unable to disclose this information. We... A BCA protein assay will likely not provide an accurate concentration for our antibodies formulated in glycerol, due to the presence of BSA and incompatibility with a higher percentage of glycerol. Our CUT&RUN Assay Kit is optimized for use with whole cells and works with a large number of cell lines, both adherent and suspension. While it is not typically necessary to pre-isolate cell nucle... Please see Maniaci, C. et al. (2017) Nat. Commun. 8, 830 (PMID: 29018234; https://pubmed.ncbi.nlm.nih.gov/29018234/) and Frost, J. et al. (2021) J. Biol. Chem. 297, 100910 (PMID: 34174286; We do not recommend our RIPA Buffer (10X) #9806 for Native PAGE as it is too stringent. Unfortunately we do not carry a buffer appropriate for Native PAGE. The CUT&RUN Antibody Binding Buffer #15338, CUT&RUN 10X Wash Buffer #31415, and diluted CUT&RUN 1X wash buffer are stable at RT or 4C for one month. However, the Digitonin Solution #163... We have several monoclonal antibodies for detecting ribosomal protein S6 that are validated for IF-IC with human samples. These are S6 Ribosomal Protein (5G10) Rabbit mAb #2217 and S6 Ribosomal Pro... We have several Lck antibodies validated for use in western blotting applications. They are as follows: The most sensitive antibody will depend on whether you are trying to detect Cas9 protein from Streptococcus pyogenes, Staphylococcus aureus, or Campylobacter jejuni. The Cas9 (7A9... Any tissue shown to be positive for each marker via chromogenic IHC can serve as a positive control tissue for a SignalStar™ assay. Each target will therefore require a positive control, which may som... YAP and TAZ are co-expressed abundantly in every cell line that has been shown to be positive for either protein, and likewise, they are negative/low in the same cell lines (essentially, they appear t... PTMScan® is compatible with the use of stable isotope labeling using amino acids in cell culture, or SILAC. The incorporation of heavy stable isotopes has no effect ... Our products can be expected to perform at optimal levels up until the printed "Best" by date on the vial when stored under the recommended conditions. Many of our products will work well af... The methods for the LC and MS are shown below:Why is it important to include both positive and negative controls in your IF experiment?
How does the specificity of the F(ab’)2 fragments differ from the corresponding full-length monoclonal antibodies?
What is the concentration of the PTMScan® Ubiquitin Branch Motif (K-ε-GG) Immunoaffinity Beads #1990 component in the PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Kit #5562?
Can I use a BCA assay to check the concentration of my antibody?
Does your CUT&RUN Assay Kit work with isolated nuclei?
What are the three bands detected by western blot with your VHL Antibody #68547?
Can I use your RIPA Buffer (10X) #9806 for Native PAGE?
What is the stability of the CUT&RUN Antibody Binding Buffer #15338 and the CUT&RUN 10X Wash Buffer #31415?
Which S6 ribosomal protein antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
Which Lck antibody do you suggest for my western blot experiment?
Which Cas9 antibody is the most sensitive by western immunoblot?
What is an appropriate positive control to include in this SignalStar™ assay? Are multiple controls necessary?
Will the Non-phospho (Active) YAP (Ser127) (E6U8Z) Rabbit mAb #29495 cross-react with TAZ?
Is PTMScan® compatible with SILAC?
Is the “Best By” date the expiration date for the product? Will the product still work after that date?
What are the methods for PTMScan® mass spectrometry?