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How should I resuspend and aliquot the PTMScan® LysC Protease #84748?

To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, ...

Why do I need to use the three buffers in the Cell Fractionation Kit #9038?

Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic deterg...

Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?

Yes, please select “Human” in the first step of the SignalStar Multiplex IH...

How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?

We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reage...

Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??

Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&a...

Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&RUN experiments?

The Concanavalin A beads can clump together rather easily, especially when the cells become lysed. To avoid this, make sure your cells are healthy and be sure to treat them very ge...

Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?

The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 h...

How do the Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 and the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 compare in terms of their sensitivity and specificity?

The Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 is a mouse mAb from a single clone, whereas the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 is a mix of rabbit mAb...

How does the specificity of the F(ab’)2 fragments differ from the corresponding full-length monoclonal antibodies?

Since the paratopes of the antibody fragments are identical to that of the corresponding full-length monoclonal antibodies, their specificity for scFv linkers remains unchang...

In terms of cytokine activity, what is the difference between International Units (IU) and specific activity?

International Units (IU) and specific activity are two differe...

Why is the concentration of my antibody so low?

At CST, our antibodies are formulated on the basis of their reactivity and performance for each approved application at a recommended dilution. For antibodies with high affinit...

Which phospho-AMPKα antibody do you suggest for my western blot experiment?

The selection of a phospho-AMPKα antibody depends on the subunit of interest. Cell Signaling Technology has four antibodies that target phospho-AMPKα:

  • Phospho-AMP...

    Which cleaved caspase-3 (Asp175) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?

    We have several antibodies for detecting Cleaved Caspase-3 (Asp175) that are validated for IF-IC with human samples. These are:

    • Cleaved Caspase-3 (Asp175) Antibody #9661

      Does this antibody work for my application (western blot, immunoprecipitation, immunofluorescence, immunohistochemistry, ELISA, flow cytometry, chromatin immunoprecipitation, etc.)?

      CST routinely tests antibodies in relevant applications. Those that pass rigorous application-specific validation standards are recommended. We only recommend applications that hav...

      Does CUT&RUN show a bias toward euchromatin or heterochromatin?

      We do not observe any bias toward euchromatin or heterochromatin in our assays. In CUT&RUN, the target-specific antibody recruits the pAG-MNase and directs digestion to chromat...

      What is the solution formulation of the PTMScan Control peptides?

      The solution formulation of the PTMScan Control peptides is 25% acetonitrile, 74.9% water, 0.1% trifluoroacetic acid (V:V).<...

      Which phospho-Akt (Ser473) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?

      We have several antibodies for detecting Akt phosphorylation at Ser473 that are validated for IHC-P with human samples. These are:

      • Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb...

        Why can't I add a target to the channel I want when using the SignalStar™ Multiplex IHC Panel Builder?

        During our extensive validation process, we found that some targets do not perform optimally with specific fluorophore pairings. When this is the case, the SignalStar Multiplex IHC...

        What controls should I include when performing a Simple Western™ experiment?

        In addition to a known positive control sample, we recommend the following negative controls for your Simple Western™ experiment.

        1. A no lysate control. This includes the p...

        Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) with mouse samples?

        We have several antibodies for detecting Akt phosphorylation at Ser473 that are validated for IF-IC with mouse tissues. These are:

        • Phospho-Akt (Ser473) (D9E) XP® ...

          What is the intended use of the PTMScan® Control peptides?

          These synthetic peptides are recoverable using our immunoaffinity purification (IAP) antibody beads, and thus provide users with a measure of PTM...

          What isoforms does the ZBP1 Antibody #60968 recognize?

          ZBP1 (Z-DNA binding protein 1), also referred to as DAI (DNA-dependent activator of IFN-regulatory factors) and DLM-1, is a nucleotide-binding protein that plays a role in tumorige...

          What is the intended use of PTMScan® LysC Protease #84748?

          The intended use of the PTMScan® LysC Protease #84748 is to digest proteins into peptides as input for PTMScan Kits where an arginine is part of the PTM motif sequence.

          A li...

          What controls can I use to show my CUT&RUN experiment is working?

          Whether performing CUT&RUN with downstream qPCR or NG-seq analysis, we always recommend using a CUT&RUN-validated positive control antibody like our 

          How can I confirm the PTMScan® LysC Protease #84748 digested my proteins efficiently?

          To confirm digestion, we recommend taking a ~1% aliquot of the protein sample pre- and post-digestion and running them on an SDS-PAGE gel with Coomassie blue staining. After di...

          Is Fc blocking needed for F(ab') antibody fragments used in live cell flow cytometry assays?

          No, the use of Fc block is not required when using F(ab') or F(ab')2 antibody fragments in live cell flow cytometry assays, as removal of the Fc domain eliminates the...

          Can I store the resuspended PTMScan® LysC Protease #84748 aliquots at -20°C instead of -80°C?

          Although the lyophilized powder can be stored at -20°C, we recommend long-term storage of the resuspended LysC at -80°C. We do not recommend long-term storage of the resuspended Ly...

          Which total Akt antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?

          We have several antibodies for detecting total Akt protein that are validated for IF-IC with human samples. These are as follows: