Technical Support Articles
Results (713)
Do you have a recommended protocol for stripping/re-probing blots?
The western blot stripping/re-probing protocol for our Western Blot Stripping Buffer (5X) #91925 is below. However, it is important to note that we typically advise against strippi...
Does the CST CUT&Tag kit work with isolated nuclei?
Our CUT&Tag kit is optimized for use with whole cells and works with a large number of cell lines, both adherent and suspension. While our kit works with pre-isolated nuclei be...
Does your Tau (D1M9X) XP® Rabbit mAb #46687 recognize all six Tau isoforms?
Our Tau (D1M9X) XP® Rabbit mAb #46687 is predicted to recognize 2N4R (tau40), 2N3R (tau39), 1N4R (tau46), 1N3R (tau37), 0N4R (tau24), and 0N3R (tau23), as all six isoforms include ...
Can your Streptavidin-HRP #3999 be used for ELISA?
Regrettably, we do not test our Streptavidin-HRP #3999 for use in ELISA . Therefore, we cannot provide any firm guarantees or a recommended dilution. However, it should work for EL...
Are the Protein A Magnetic Beads #73778 and the Protein G Magnetic Beads #70024 hydrophilic or hydrophobic?
The base particle of the Protein A Magnetic Beads #73778 and the Protein G Magnetic Beads #70024 is somewhat hydrophilic. It is difficult to predict the hydrophilicity or hydrophob...
What are the differences between the BrdU Cell Proliferation Chemiluminescent Assay Kit #5492 and the BrdU Cell Proliferation Assay Kit #6813? Which should I choose?
In general, the BrdU Cell Proliferation Chemiluminescent Assay Kit #5492 offers a broader dynamic range and higher sensitivity compared to the BrdU Cell Proliferation Assay Kit #68...
Why is there an additional band at 24kDa using the Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718?
The extra band(s) around 24 kDa using our Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718 are most likely mono-ubiquitylated H2AX. H2AX, like H2A, is mono-ubiquitylated.
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic deterg...
Can I order a custom formulation of an antibody?
For custom (carrier-free) orders, many antibodies are available pre-formulated in PBS without BSA or glycerol. This carrier-free formulation is amenable to conjugation and can be c...
Do your GFP antibodies detect citrine?
We have not tested any of our GFP antibodies against the citrine variant of YFP.
Why is the concentration of my antibody so low?
At CST, our antibodies are formulated on the basis of their reactivity and performance for each approved application at a recommended dilution. For antibodies with high affinit...
What is the solution formulation of the PTMScan Control peptides?
The solution formulation of the PTMScan Control peptides is 25% acetonitrile, 74.9% water, 0.1% trifluoroacetic acid (V:V).<...
Does this antibody work for my application (western blot, immunoprecipitation, immunofluorescence, immunohistochemistry, ELISA, flow cytometry, chromatin immunoprecipitation, etc.)?
CST routinely tests antibodies in relevant applications. Those that pass rigorous application-specific validation standards are recommended. We only recommend applications that hav...
Is Fc blocking needed for F(ab') antibody fragments used in live cell flow cytometry assays?
Do you have a protocol for lysing in a 96-well plate (no sonication)?
For lysis in 96-well assay plates, we recommend incubation in the provided lysis buffer for ~30 min at 4°C with gentle shaking. While sonication is difficult in small volumes, w...
Why is a fluorescent conjugate of a rabbit antibody giving no signal on compensation beads in flow cytometry?
If you are using compensation beads in your flow cytometry experiment and observe no signal with one of our fluorescent conjugates of a rabbit antibody, this may be due to the reac...
Which Cadherins does the Pan-Cadherin Antibody #4068 detect?
The Pan-Cadherin Antibody #4068 can detect R-, N-, E-, and VE-Cadherin. It does not detect P- or K-Cadherin. Reactivity to other Cadherins has not been tested.
Why do my Concanavalin A Magnetic Beads #82307 appear aggregated?
As long as your Concanavalin A Magnetic Beads #82307 have been stored at the appropriate recommended temperature, the appearance of aggregation is not a cause for concern. By natur...
Can I use human PBMCs with Senescence β-Galactosidase Staining Kit #9860?
CST scientists have not tested Senescence β-Galactosidase Staining Kit #9860 using human peripheral blood mononuclear cells (PBMCs). Therefore, we cannot provide a suggested...
Which Cas9 antibody is the most sensitive by western immunoblot?
The most sensitive antibody will depend on whether you are trying to detect Cas9 protein from Streptococcus pyogenes, Staphylococcus aureus, or Campylobacter jejun...
Are the Anti-rabbit IgG, HRP-linked Antibody #7074 and Anti-mouse IgG, HRP-linked Antibody #7076 affinity purified?
Our Anti-rabbit IgG, HRP-linked Antibody #7074 and Anti-mouse IgG, HRP-linked Antibody #7076 are purified by affinity chromatography and adsorbed to remove any cross-reactivities t...
How were the sequences of the PTMScan® Control peptides chosen?
The sequences of the synthetic peptides were based on modified peptides that we have consistently recovered and identified in human and mouse sam...
Which ribosomal protein S6 (rpS6) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several antibodies for ribosomal protein S6 (rpS6) that are validated for IHC-P with mouse samples. These are S6 Ribosomal Protein (5G10) Rabbit mAb #2217 and S6 Ribosomal ...
Can I use mixed species samples for a PTMScan® experiment?
The PTMScan® technology is compatible with samples derived from all kingdoms of life as well as samples containing mixed species like xenografts (mouse and human), microbiome sampl...
Does the Cellular Glutathione Detection Assay Kit #13859 detect both reduced and oxidized glutathione?
The Cellular Glutathione Detection Assay Kit #13859 detects only the reduced form of glutathione. It is unable to detect the oxidized form.
What is the concentration of the pAG-MNase Enzyme #57813?
The concentration of the pAG-MNase Enzyme #57813 in our CUT&RUN kits is 25ug/ml.
When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. How do I know if this excess staining is correct?
During the course of optimization, we’ve found that fluorescent staining may show higher %-positivity than chromogenic staining. To ensure any excess staining is specific, confirm ...
Can the Phospho-β-Catenin (Ser33/37) Antibody #2009 bind if the sites are singly phosphorylated or is the phosphorylation at both sites necessary?
We have tested the reactivity of the Phospho-β-Catenin (Ser33/37) Antibody #2009 to Ser33/37 by peptide dot blot. This antibody showed strong signal when phosphorylated at Ser37 al...
What is the extinction coefficient of my rabbit antibody?
The molar extinction coefficient of rabbit IgG at 280 nm is approximately 210,000 M-1cm-1.
How much sample is required for a PTMScan® experiment?
We recommend using 10 mg of protein per immunoaffinity purification (IAP) sample for our standard PTMScan kits. For the PTMScan HS product line, 1 mg of starting material is optima...