Technical Support Articles
Results (714)
I need guidance on optimizing the performance of the SignalFlex™ conjugate within my assay. Who should I contact?
Please reach out to our Technical Support Team for guidance and s...
How are SignalFlex™ conjugates different from custom-ordered antibody conjugates?
Both SignalFlex and custom-ordered conjugates are made after purchase order and are manufactured using the same high-quality standards and methods as off-the-shelf CST conjugate...
Which mouse-reactive PD-L1 clones are most sensitive by WB and IHC?
We have found that the F3Z1T clone is the most sensitive clone for detecting mouse PD-L1. This clone can be found in the following products that are approved for Western blottin...
What are the differences between the four ECL Reagents for Western blot chemiluminescent detection: #7003, #6883, #12757, and #12630?
All four ECL reagents for Western blot chemiluminescent detection work well for HRP detection. However, each reagent has its own level of sensitivity and detection limits:
...Which of your epidermal growth factor receptor (EGFR) antibodies will avoid competition with Cetuximab binding?
Cetuximab binds to extracellular domain III of human epidermal growth factor (EGF) receptor, which represents amino acids 310-480. The best antibody product in our catalog with ...
Which mouse-reactive PD-L1 antibody is best for western blotting?
Cell Signaling Technology currently offers four mouse-reactive antibodies targeting PD-L1:
- PD-L1 (F3Z1T) Rabbit mAb #73552
- PD-L1 (D4H1Z) Rabbit mAb #60475
What applications work well with SignalFlex™ conjugates?
Although SignalFlex conjugates have not been tested in specific applications, it is helpful to review the applications that the unconjugated parent antibody, and similar conjuga...
Why do your Glucocorticoid Receptor (D6H2L) XP® #12041 and Glucocorticoid Receptor (D8H2) XP® #3660 Rabbit mAbs detect two bands at 91 and 94 kDa?
From our testing, we have seen that our Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb #12041 detects both the alpha and beta isoforms of the Glucocorticoid Receptor. It is possibl...
Can I get assistance with my panel design and how to incorporate SignalFlex™ conjugates into my multiplex flow cytometry experiment?
We have several tools that may be helpful when designing your panel. These include:
What isotype control should I use in my SignalFlex™ experiement?
Compatible isotype controls are available in our off-the-shelf CST catalog. Choose an isotype control with the same dye as the SignalFlex conjugate(s) you use.
Why do I see a doublet band when probing with Rpb1 NTD (D8L4Y) Rabbit mAb #14958 for Western blot?
The two bands detected by our Rpb1 NTD (D8L4Y) Rabbit mAb (#14958) represent hyperphosphorylated (upper) and non-phosphorylated/hypophosphorylated (lower) Rpb1 (see PMID: 185888...
I need a larger quantity of SignalFlex™ conjugate for my experiments. How can I order more than 50 μg?
You can order more than 50 μg of your SignalFlex conjugate by purchasing multiple vials of the product. If your project requires a large amount from a single manufacturing lot, ...
Will the IgG (RWP49) Mouse mAb #33469 cross-react with monkey tissues?
The immunization strategy used to create IgG (RWP49) Mouse mAb #33469 gives a high likelihood of reactivity with all G1 isotypes. We do not have any formal data with regard to t...
How can you ensure SignalFlex™ conjugates are consistent from lot to lot?
At CST, scientists make over 95% of our antibodies in-house. Because we control our antibody manufacturing process and rigorous conjugation procedures in-house, our scientists e...
What is the optimal concentration of SignalFlex™ conjugates to use in my experiment?
The best concentration of SignalFlex antibody conjugate will vary depending on the application and the sample or model system used. Researchers should first test multiple titrat...
Where can I learn more about the spectral properties of the fluorescent dyes offered with the SignalFlex™ conjugates?
Use the Fluorescence Spectra Viewer to view and better understand the...
What positive and negative controls can I use to demonstrate that my CUT&Tag experiment is successful?
We recommend using a CUT&Tag-validated positive control antibody, like our Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751, in your experiment to confirm that your CUT&am...
Why aren't SignalFlex™ conjugates tested in specific applications to determine optimal concentrations before shipping?
SignalFlex conjugates are manufactured on demand, specifically for your order. This allows us to offer a wider range of dye conjugate combinations while expediting the delivery ...
Why is there an additional band at 24kDa using the Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718?
The extra band(s) around 24 kDa using our Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718 are most likely mono-ubiquitylated H2AX. H2AX, like H2A, is mono-ubiquitylated.
Why do I see a doublet with my beta-Actin antibody?
It is not uncommon to see a doublet with our beta-Actin antibodies depending on the samples being tested. It is likely caused either by phosphorylation of beta-Actin on its many ph...
Why does c-Jun migrate at a higher molecular weight after treatment?
The transcriptional activity of c-Jun is regulated by SAPK/JNK phosphorylation at Ser63 and Ser73. The signaling pathway can be found at https://www.cellsignal.com/contents/science...
I cannot complete the BrdU cell proliferation assay in the given time. Is there a step where I can stop?
We recommend that you complete the BrdU cell proliferation assay as soon as you have the time. However, if you are unable to complete the experiment during the recommended time,...
What size are your Protein A Agarose Beads #9863?
Our Protein A Agarose Beads #9863 have a diameter of 45 - 165um. They are supplied in a 50% slurry and the bead matrix is 6% agarose cross-linked.
Why do I see multiple bands with your c-Myc antibodies?
Multiple bands may be observed when detecting c-Myc because there are multiple isoforms of c-Myc and c-Myc undergoes several potential post-translational modifications (e.g., phosp...
I don’t see the antibody-dye combination I’m looking for. When will it be available?
Our off-the-shelf and SignalFlex™ conjugate catalogs continue to grow. While new off-the-shelf antibody conjugates are added weekly and new SignalFlex conjugates a...
Where can I find the RRID numbers for CST antibodies?
RRIDs (Research Resource Identifiers) can be found in the RRID Portal (https://rrid.site/). Simply search for the product number...
What is your policy on canceling SignalFlex™ conjugate orders?
We are unable to accept cancellations for SignalFlex conjugate orders once they have been placed. This is due to the fact that production begins immediately after your order has...
Will the β-Actin Antibody #4970 detect both full-length β-actin as well as the cleaved fragments?
β-actin can be cleaved by caspase-3 between Asp244 and Gly245, which will result in a 32kDa fragment and a 15kDa fragment. Due to the location of its antigen, our β-Actin Antibody ...
How are SignalFlex™ conjugates different from other off-the-shelf CST antibody conjugates?
SignalFlex conjugates are similar in that they are manufactured using the same high-quality standards and specifications as our other off-the-shelf CST antibody conjugates. They...