Technical Support Articles
Results (713)
Can I use your Protein A Agarose Beads #9863 and Protein G Agarose Beads #37478 if they have been frozen?
Although we are unable to guarantee that both our Protein A Agarose Beads #9863 and Protein G Agarose Beads #37478 will work after being stored at -20C, we have used beads that hav...
Why doesn't PD-L2 migrate at 31 kDa?
The predicted molecular weight of PD-L2 is 31 kDa (based on amino acid composition). However, in most models, PD-L2 migrates at an observed molecular weight of 45-60 kDa on Tris-Gl...
What is the expected CUT&RUN DNA yield when starting with extremely low cell numbers, such as 5,000 - 10,000 cells?
If you start with 100,000 cells, you typically get 0.6-6ng DNA. However, if you start with 5,000 - 10,000 cells, the DNA yield can be as low as 0.1-0.2 ng or even undetectable by t...
Can I quantify my samples using the Senescence β-Galactosidase Staining Kit #9860?
The Senescence β-Galactosidase Staining Kit #9860 is intended to be a qualitative assay to determine if cells are senescent. We have not determined a means of quantification in-hou...
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with human samples. These are:
- Cleaved Caspase-3 (Asp175) Antibody #9...
If your Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb #6943 detects human Src protein when phosphorylated at Tyr419, why does the product name say Tyr416?
Our Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb #6943 detects endogenous levels of human Src protein only when phosphorylated at Tyr419. The discrepancy in the product name is ...
Can you provide data that confirms the specificity of your BACE (D10E5) Rabbit mAb #5606?
The specificity of our BACE (D10E5) Rabbit mAb #5606 has been confirmed in the literature on brain extracts prepared from BACE1 (-/-) mice. Please see Figure 1 in Shahani, N. et al...
Where can I find example data for your PTMScan® kits?
We provide example data for our PTMScan enrichment kits on this webpage:
https://www.cellsignal.com/applications/proteomics/ptmscan-motif-antibody-kits
To the right o...
What is the difference between your SimpleChIP® ChIP-seq Single Index Primers #29580 and SimpleChIP® ChIP-seq Dual Index Primers #47538?
Both SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Single Index Primers) #29580 and SimpleChIP® ChIP-seq Multiplex Oli...
What isoforms does the TGF-β Antibody #3711 detect and will it detect the 25kDa mature form of TGF-β?
The TGF-β Antibody #3711 detects recombinant TGF-β1, TGF-β2, and TGF-β3. The antibody also detects endogenous levels of the TGF-β1 precursor proteins. The different sizes we have l...
How do I lyse my cells for the Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) #23833?
What isoforms are detected by your β-Actin (13E5) Rabbit mAb #4970?
Our β-Actin (13E5) Rabbit mAb #4970 detects endogenous levels of total β-actin protein. Despite the high sequence identity between the cytoplasmic actin isoforms, β-actin and cytop...
Does the amino acid sequence of SARS-CoV-2 Spike RBD (multimeric) (319-591) Recombinant Protein (8xHis-Tag) #17862 (319-591) being longer than the amino acid sequence of other RBD products (318-541) affect its binding activity with ACE2?
The additional amino acids found in SARS-CoV-2 Spike RBD (multimeric) (319-591) Recombinant Protein (8xHis-Tag) #17862 should not affect the binding of RBD to ACE2 bec...
Can the Phospho-β-Catenin (Ser33/37) Antibody #2009 bind if the sites are singly phosphorylated or is the phosphorylation at both sites necessary?
We have tested the reactivity of the Phospho-β-Catenin (Ser33/37) Antibody #2009 to Ser33/37 by peptide dot blot. This antibody showed strong signal when phosphorylated at Ser37 al...
How do I prepare the samples shown in the web image for your Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) #23833?
The samples shown in the web image for our Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) #23833 were prepared as follows:
1. Treat cells with 200 nM ...
Can I use RIPA Buffer (10X) #9806 for ELISA experiments?
Our ELISA kits are compatible with our RIPA Buffer (10X) #9806, however, this bu...
Can I purchase Solution B outside of the Senescence β-Galactosidase Staining Kit #9860?
We are currently unable to provide the Solution B component separately from the Senescence β-Galactosidase Staining Kit #9860. However, we can disclose the ingredients of Solution ...
Why should I use your recommended protocol or reagents for my IHC experiment?
We have performed a number of comparisons over the years looking at protocol variables such as the retrieval solution, diluent, incubation, and detection methodology. We have seen ...
Can I use the Cyclic AMP XP® Assay Kit #4339 with tissue samples?
We have not tested tissue samples in-house using the Cyclic AMP XP Assay Kit #4339, so although we cannot guarantee it will work, there is no reason it should not. We recommend loa...
What are the high molecular weight bands detected by the β1-Adrenergic Receptor Antibody #12271?
The antigen for the β1-Adrenergic Receptor Antibody #12271 surrounds Gly406 of the human β1-Adrenergic Receptor protein and is contained entirely in the cytoplasmic domain. #12271 ...
Will your Anti-biotin, HRP-linked Antibody #7075 cross-react with endogenous biotin in cell lysates?
Our Anti-biotin, HRP-linked Antibody #7075 is intended for use with our Biotinylated Protein Ladder Detection Pack #7727. However, it is possible for this antibody to also detec...
Are the light chains of the β-Actin (8H10D10) Mouse mAb #3700 kappa or lambda?
The β-Actin (8H10D10) Mouse mAb #3700 has kappa light chains.
What are the differences between the BrdU Cell Proliferation Chemiluminescent Assay Kit #5492 and the BrdU Cell Proliferation Assay Kit #6813? Which should I choose?
In general, the BrdU Cell Proliferation Chemiluminescent Assay Kit #5492 offers a broader dynamic range and higher sensitivity compared to the BrdU Cell Proliferation Assay Kit #68...
What samples and species were tested with the Griess Reagent Nitrite Measurement Kit #13547?
We have tested the Griess Reagent Nitrite Measurement Kit #13547 with media, cell culture supernatant, and urine. It should cross-react with all species types.
Why is there a difference in the amino acid region of Human ACE2 (18-652) Recombinant Protein (mFc-Tag) #83986 and Human ACE2 (18-615) Recombinant Protein (8xHis-Tag) #73775?
The slight difference in the amino acid span of these products is not significant for binding to SARS-CoV-2. The Human ACE2 (18-652) Recombinant Protein (mFc-Tag) #839...
Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Human” in the first step of the SignalStar Multiplex IH...
Can you convert the OD readout to the number of viable cells from the XTT Cell Viability Kit #9095?
We have not back calculated the number of viable cells per well based on the OD readout from the XTT Cell Viability Kit #9095. We use the OD readout to see the activity level of th...
Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?
We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include:
- β-Actin (13E5) Rabbit mAb #4970
- β-Actin...
What is the binding capacity of the antibody-bead conjugates for the agarose PTMScan® kits?
The background binding of non-PTM peptides is typically 50% or more of the total peptides bound to the antibody beads in our agarose PTMScan® kits. This has been improved to less t...
Why is the concentration of my antibody so low?
At CST, our antibodies are formulated on the basis of their reactivity and performance for each approved application at a recommended dilution. For antibodies with high affinit...