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Technical Support Articles

Results (709)

Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?

Yes, please select “Mouse” in the first step of the SignalStar Multiplex IH...

My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?

Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are st...

What is an appropriate positive control to include in this SignalStar™ assay? Are multiple controls necessary?

Any tissue shown to be positive for each marker via chromogenic IHC can serve as a positive control tissue for a SignalStar™ assay. Each target will therefore require a positive co...

When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. How do I know if this excess staining is correct?

During the course of optimization, we’ve found that fluorescent staining may show higher %-positivity than chromogenic staining. To ensure any excess staining is specific, confirm ...

How long after the completion of staining can I wait to image my slides when performing a SignalStar™ Multiplex IHC assay?

For Imaging Round 1, the staining should show robust signal when imaged up to 8 hours post completion of staining. For Imaging Round 2, imaging should be performed as close to the ...

Can my slides be used for other assays after SignalStar™ Multiplex IHC?

SignalStar™ Multiplex IHC Kits & Reagents have not been validated for use in combination with other assays. The SignalStar technology does not destroy the tissue, so it may be ...

Is PTMScan® compatible with SILAC?

PTMScan® is compatible with the use of stable isotope labeling using amino acids in cell culture, or SILAC. The incorporation of heavy stable iso...

What is the extinction coefficient of my rabbit antibody?

The molar extinction coefficient of rabbit IgG at 280 nm is approximately 210,000 M-1cm-1.

Can I autoclave the 6-Tube Magnetic Separation Rack #7017?

No, these racks cannot be autoclaved. Exposing them to high autoclave temperatures may cause the magnets to lose their magnetism. Instead, we suggest submerging or spraying the ...

Which nuclear dyes can be used for In-Cell Western assays in combination with DyLight 680-conjugated secondary antibodies?

When performing In-Cell Western assays with DyLight 680-conjugated secondary antibodies, we recommend using either Hoechst 33342 or DAPI as a nuclear dye. DRAQ5 cannot be used beca...

Have species been tested if they are not listed as cross-reactive with a CST antibody on the datasheet?

Possibly. CST scientists test all our antibodies on multiple species when feasible, depending on the target protein and the experimental systems available. If a tested species does...

Which caspase-3 antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?

We have two antibodies for detecting caspase-3 that are validated for IHC-P with human samples, Caspase-3 Antibody #9662 and Caspase-3 (D3R6Y) Rabbit mAb (IHC Formulated) #14214. W...

Can I combine antibodies used in a SignalStar™ assay with direct conjugates?

SignalStar Multiplex IHC kits and reagents have been validated for use in combination with direct conjugates. The SignalStar™ Fluorescence Removal Kit 

Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) (IF-IC) with human samples?

We have several antibodies for detecting the phosphorylation of Akt at Ser 473 that are validated for IF-IC with human samples. These include the following: