Technical Support Articles
Results (694)
What is the concentration of the pAG-MNase Enzyme #57813?
The concentration of the pAG-MNase Enzyme #57813 in our CUT&RUN kits is 25ug/ml.
Can you provide data that confirms the specificity of your BACE (D10E5) Rabbit mAb #5606?
The specificity of our BACE (D10E5) Rabbit mAb #5606 has been confirmed in the literature on brain extracts prepared from BACE1 (-/-) mice. Please see Figure 1 in Shahani, N. et al. (2014 ) J Biol Che...
How should I store the XTT Cell Viability Kit #9095 for future use?
We have performed several freeze/thaw experiments and have found that the reagents of the XTT Cell Viability Kit #9095 are very stable after 5 freeze/thaw cycles. It is also fine to aliquot the reagen...
Can the SARS-CoV-2 Spike Protein Serological IgG ELISA Kit #20154 be used for quantitation?
The SARS-CoV-2 Spike Protein Serological IgG ELISA Kit #20154 kit is meant to be used to determine if the sample is positive or negative for SARS-CoV-2 and not for relative quantitation. The included ...
My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?
Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex...
What are common considerations for using the Senescence β-Galactosidase Staining Kit #9860?
Some common considerations for using the Senescence β-Galactosidase Staining Kit #9860 are as follows:
1. Please ensure that the 10X Staining Solution, as well as Solution A and B, are fully...
Can I combine antibodies used in a SignalStar™ assay with direct conjugates?
SignalStar Multiplex IHC kits and reagents have been validated for use in combination with direct conjugates. The SignalStar™ Fluorescence Removal Kit When selecting an antibody for use in CUT&RUN, we recommend starting with a ChIP or ChIP-seq-validated antibody; however, we have found that not all ChIP-validated antibodies work in the CUT&am... We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with human samples. These are:
What is the best way to choose an antibody for my CUT&RUN assay if I cannot find a CUT&RUN validated antibody for my target protein?
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Mouse” in the first step of the SignalStar Multiplex IHC Panel Builder ...
Which phospho-AMPKα antibody do you suggest for my western blot experiment?
The selection of a phospho-AMPKα antibody depends on the subunit of interest. Cell Signaling Technology has four antibodies that target phospho-AMPKα:
- Phospho-AMPKα (Thr172) (40H9) R...
When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. How do I know if this excess staining is correct?
During the course of optimization, we’ve found that fluorescent staining may show higher %-positivity than chromogenic staining. To ensure any excess staining is specific, confirm that the correct sub...
Which cleaved caspase-3 (Asp175) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
We have several antibodies for detecting Cleaved Caspase-3 (Asp175) that are validated for IF-IC with human samples. These are:
- Cleaved Caspase-3 (Asp175) Antibody #9661
- Cleaved Caspas...
I don’t see my target of interest in your menu of available antibodies for SignalStar™ Multiplex IHC. Can I still use it in my panel in some way?
SignalStar Multiplex IHC kits and reagents haven’t yet been validated for use with antibodies outside of our menu. We’re in the process of developing cust...
Does CUT&RUN show a bias toward euchromatin or heterochromatin?
We do not observe any bias toward euchromatin or heterochromatin in our assays. In CUT&RUN, the target-specific antibody recruits the pAG-MNase and directs digestion to chromatin that is directly ...
What controls can I use to show my CUT&RUN experiment is working?
Whether performing CUT&RUN with downstream qPCR or NG-seq analysis, we always recommend using a CUT&RUN-validated positive control antibody like our Any tissue shown to be positive for each marker via chromogenic IHC can serve as a positive control tissue for a SignalStar™ assay. Each target will therefore require a positive control, which may som... Product Iba1/AIF-1 (E4O4W) XP® Rabbit mAb #17198 is predicted to detect isoform 1 and isoform 2 of the Ionized calcium-binding adaptor molecule 1 (Iba1), also known as allograft inflammator... Tagmentation takes place within the nuclei. To minimize non-specific background, any excess Tn5 enzyme is washed away prior to activation. Similarly, spike-in DNA introduced into the reaction woul... SignalStar™ Multiplex IHC Kits & Reagents have not been validated for use in combination with other assays. The SignalStar technology does not destroy the tissue, so it may be possible to perform ... Yes. We have shown that our CUT&Tag Assay Kit #77552 works with mouse liver, mouse brain, and mouse heart tissues for histone targets with as little as 1 mg of tissue for each CUT&Tag reaction... We have several antibodies for detecting the phosphorylation of Akt at Ser 473 that are validated for IF-IC with human samples. These include the following:
What is an appropriate positive control to include in this SignalStar™ assay? Are multiple controls necessary?
Which isoforms does the ba1/AIF-1 (E4O4W) XP® Rabbit mAb #17198 detect?
Why can't spike-in DNA be used in CUT&Tag assays?
Can my slides be used for other assays after SignalStar™ Multiplex IHC?
Is it possible to perform CUT&Tag on samples like heart, brain, or developing mouse tissues?
Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) (IF-IC) with human samples?
Is PTMScan® compatible with SILAC?
PTMScan® is compatible with the use of stable isotope labeling using amino acids in cell culture, or SILAC. The incorporation of heavy stable isotopes has no effect ...
Why do I see a doublet with my beta-Actin antibody?
It is not uncommon to see a doublet with our beta-Actin antibodies depending on the samples being tested. It is likely caused either by phosphorylation of beta-Actin on its many phosphorylation sites,...
Which nuclear dyes can be used for In-Cell Western assays in combination with DyLight 680-conjugated secondary antibodies?
When performing In-Cell Western assays with DyLight 680-conjugated secondary antibodies, we recommend using either Hoechst 33342 or DAPI as a nuclear dye. DRAQ5 cannot be used because the emission spe...
What is the extinction coefficient of my rabbit antibody?
The molar extinction coefficient of rabbit IgG at 280 nm is approximately 210,000 M-1cm-1.
Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&Tag experiments?
Concanavalin A beads can clump together rather easily, especially when cells become lysed. Make sure your cells are healthy and be sure to treat them very gently during the cell wash. We also recommen...
Which cleaved caspase-3 (Asp175) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with mouse samples?
We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IF-IC with mouse samples. These are:
- Cleaved Caspase-3 (Asp175) Antibody #9661
- Cleaved ...
Why isn't my bottle of Cell Lysis Buffer (10X) #9803 frozen?
Occasionally, Cell Lysis Buffer (10X) #9803 stored at -20C may not appear frozen, which is not a reason for concern. This phenomenon is known as nucleation, or the initial formation of ice from a liqu...
What are the two bands seen in western blot analysis using ANKRD15 (E9I4I) Rabbit mAb #69953?
When using ANKRD15 (E9I4I) Rabbit mAb #69953, two distinct bands are typically observed in western blotting experiments. These bands represent two different isoforms of the ANKRD15 protein:
- I...