Cell Signaling Technology Logo - Extra Large

Technical Support Articles

Results (694)

How does the specificity of the F(ab’)2 fragments differ from the corresponding full-length monoclonal antibodies?

Since the paratopes of the antibody fragments are identical to that of the corresponding full-length monoclonal antibodies, their specificity for scFv linkers remains unchanged. However, the abs...

Is the “Best By” date the expiration date for the product? Will the product still work after that date?

Our products can be expected to perform at optimal levels up until the printed "Best" by date on the vial when stored under the recommended conditions. Many of our products will work well af...

What is mouse on mouse (MOM) background in IHC and how can it be addressed?

"Mouse on mouse" (MOM) background is caused by the anti-mouse secondary antibody binding to endogenous mouse IgG expressed in the tissue resulting in non-specific staining, which can complic...

Why do your Rad18 antibodies detect two bands by western blot?

We believe the upper band observed when using the Rad18 (D2B8) XP® Rabbit mAb #9040, Phospho-Rad18 (Ser403) (D2T6W) Rabbit mAb #14978, or Phospho-Rad18 (Ser403) Antibody #8393 is likely mono-ubiquitin...

Do your IRAK4 Antibody #4363 and IRAK-M Antibody #4369 cross-react with other IRAK family members?

The antigenic peptides used to produce our IRAK4 Antibody #4363 and our IRAK-M Antibody #4369 have been carefully selected to avoid cross-reactivity with the other IRAK family members. Our IRAK-M Anti...

What is the concentration of the pAG-MNase Enzyme #57813?

The concentration of the pAG-MNase Enzyme #57813 in our CUT&RUN kits is 25ug/ml.

Can the Phospho-β-Catenin (Ser33/37) Antibody #2009 bind if the sites are singly phosphorylated or is the phosphorylation at both sites necessary?

We have tested the reactivity of the Phospho-β-Catenin (Ser33/37) Antibody #2009 to Ser33/37 by peptide dot blot. This antibody showed strong signal when phosphorylated at Ser37 alone and when phospho...

Are LICOR buffers compatible with CST products in fluorescent western blot?

While we strongly urge customers to continue using our recommended blotting buffers, it has been found that the TBS based LICOR buffers also appear to be compatible with our products in the fluorescen...

What is the benefit of carrier vs. carrier free cytokines?

Our cytokines and growth factors are lyophilized and supplied either with 20ug BSA per 1ug of cytokine (with carrier) or without added protein (carrier free). BSA minimizes adsorption to surfaces and ...

Why are the beads from PTMScan® HS Kits aggregating against the sides of the tube?

Through in-house testing, we have found that the magnetic beads from our PTMScan HS kits aggregate and stick to the walls of some specific brands of tubes. Moreover, they cannot be washed off or spun ...

Can the Lambda Protein Phosphatase Kit #89726 be stored at -20°C?

The Lambda Protein Phosphatase #56206 included in the Lambda Protein Phosphatase Kit #89726 is temperature sensitive. Therefore, we recommend storing the kit at -80°C. However, the kit is stable at -2...

What secondary antibodies do you recommend for fluorescent western blotting?

We currently have four different secondary antibodies approved for use in fluorescent western blotting, depending on species and wavelength of detection: