Technical Support Articles

Does your CUT&RUN Assay Kit work with isolated nuclei?

Our CUT&RUN Assay Kit is optimized for use with whole cells and works with a large number of cell lines, both adherent and suspension. While it is not typically necessary to pr...

Does the Human IL-22 Recombinant Protein #74808 contain any human or animal material and is it free of HIV/AIDS, HCV/HBC or other infectious material?

No materials of animal origin are used in the production of our Human IL-22 Recombinant Protein #74808. Therefore, additional testing for infectious material is not relevant.

Does your Anti-mouse IgG, HRP-linked Antibody #7076 cross react with mouse IgM?

Our Anti-mouse IgG, HRP-linked Antibody #7076 shows approximately 25% reactivity to mouse IgM.

What is the concentration of the pAG-MNase Enzyme #57813?

The concentration of the pAG-MNase Enzyme #57813 in our CUT&RUN kits is 25ug/ml.

Why do I see high background by western blot when probing for phospho-AMPK (Thr172)?

If CST recommended buffers are being used for western blotting and high background or additional bands persist when probing for phospho-AMPK (Thr172), the issue could be related to...

Does the Cellular Glutathione Detection Assay Kit #13859 detect both reduced and oxidized glutathione?

The Cellular Glutathione Detection Assay Kit #13859 detects only the reduced form of glutathione. It is unable to detect the oxidized form.

Can I use your Protein A Agarose Beads #9863 and Protein G Agarose Beads #37478 if they have been frozen?

Although we are unable to guarantee that both our Protein A Agarose Beads #9863 and Protein G Agarose Beads #37478 will work after being stored at -20C, we have used beads that hav...

What payment methods do you accept?

For our US based customers, we accept purchase orders for all ordering options, as well as credit card payments for orders placed online or by phone. We currently accept Visa, Mast...

Where can I find example data for your PTMScan® kits?

We provide example data for our PTMScan enrichment kits on this webpage:

https://www.cellsignal.com/applications/proteomics/ptmscan-motif-antibody-kits

To the right o...

Why do you include BSA in the storage buffer for most of your off-the-shelf antibodies?

Antibodies, and proteins in general, are less susceptible to degradation when stored at higher concentrations (i.e., greater than or equal to 1.0 mg/ml). Therefore, because our off...

Can you provide data that confirms the specificity of your BACE (D10E5) Rabbit mAb #5606?

The specificity of our BACE (D10E5) Rabbit mAb #5606 has been confirmed in the literature on brain extracts prepared from BACE1 (-/-) mice. Please see Figure 1 in Shahani, N. et al...

Why is it important to include both positive and negative controls in your IF experiment?

Positive and negative controls are at the heart of any good experiment. Without them, it is difficult to assess root causes when troubleshooting. Positive controls should be includ...

Can the Phospho-β-Catenin (Ser33/37) Antibody #2009 bind if the sites are singly phosphorylated or is the phosphorylation at both sites necessary?

We have tested the reactivity of the Phospho-β-Catenin (Ser33/37) Antibody #2009 to Ser33/37 by peptide dot blot. This antibody showed strong signal when phosphorylated at Ser37 al...

Do I need to sonicate my genomic DNA for use in MeDIP and dot blot?

If your DNA fragments are larger than 500 base pairs, we recommend sonicating to form 200-500 base pair fragments that will more readily denature. Sonication should be performed pr...

What are the high molecular weight bands detected by the β1-Adrenergic Receptor Antibody #12271?

The antigen for the β1-Adrenergic Receptor Antibody #12271 surrounds Gly406 of the human β1-Adrenergic Receptor protein and is contained entirely in the cytoplasmic domain. #12271 ...

Can I use RIPA Buffer (10X) #9806 for ELISA experiments?

How do you make the 4X RNA Denaturing Buffer for RNA dot blot?

4X RNA Denaturing Buffer Recipe for use with our RNA dot blot protocol:

657ul Formamide @99.5%
210ul of 37% Formaldehyde
133ul of 10x MOPS buffer

Final formulat...

Can I use the Cyclic AMP XP® Assay Kit #4339 with tissue samples?

We have not tested tissue samples in-house using the Cyclic AMP XP Assay Kit #4339, so although we cannot guarantee it will work, there is no reason it should not. We recommend loa...

What samples and species were tested with the Griess Reagent Nitrite Measurement Kit #13547?

We have tested the Griess Reagent Nitrite Measurement Kit #13547 with media, cell culture supernatant, and urine. It should cross-react with all species types.

What is the binding capacity of the antibody-bead conjugates for the agarose PTMScan® kits?

The background binding of non-PTM peptides is typically 50% or more of the total peptides bound to the antibody beads in our agarose PTMScan® kits. This has been improved to less t...

What isoforms does the TGF-β Antibody #3711 detect and will it detect the 25kDa mature form of TGF-β?

The TGF-β Antibody #3711 detects recombinant TGF-β1, TGF-β2, and TGF-β3. The antibody also detects endogenous levels of the TGF-β1 precursor proteins. The different sizes we have l...

Does Cell Lysis Buffer (10X) #9803 work with the Bradford assay?

Cell Lysis Buffer (10X) #9803 will interfere with the Bradford protein assay. This is because it contains detergent. To make the assay somewhat compatible, dilute your protein quan...

Do your PD-1 and PD-L1 antibodies recognize the intracellular or extracellular domain?

The regions recognized by our PD-1 and PD-L1 antibodies are as follows:

PD-1 (EH33) Mouse mAb (IHC-Specific) #43248 binds the extracellular domain.
PD-1 (D7D5W) XP® Rabbi...

Are the Protein A Magnetic Beads #73778 and the Protein G Magnetic Beads #70024 hydrophilic or hydrophobic?

The base particle of the Protein A Magnetic Beads #73778 and the Protein G Magnetic Beads #70024 is somewhat hydrophilic. It is difficult to predict the hydrophilicity or hydrophob...

Which S6 ribosomal protein antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?

We have several antibodies for detecting the phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 that are validated for IHC-P with human samples. These are S6 Ribosomal Pr...

What are the differences between the BrdU Cell Proliferation Chemiluminescent Assay Kit #5492 and the BrdU Cell Proliferation Assay Kit #6813? Which should I choose?

In general, the BrdU Cell Proliferation Chemiluminescent Assay Kit #5492 offers a broader dynamic range and higher sensitivity compared to the BrdU Cell Proliferation Assay Kit #68...

What PD-L2 antibodies do you currently offer?

We currently offer three products targeting PD-L2:

Our PD-L2 (D7U8C) XP® Rabbit mAb #82723 recognizes endogenous levels of total human PD-L2 protein and was produced by immu...

Will your Anti-biotin, HRP-linked Antibody #7075 cross-react with endogenous biotin in cell lysates?

Our Anti-biotin, HRP-linked Antibody #7075 is intended for use with our Biotinylated Protein Ladder Detection Pack #7727. However, it is possible for this antibody to also detec...

How are the SignalStar™ Multiplex IHC Kits & Reagents validated?

Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&a...