Technical Support Articles
Results (709)
Can I autoclave the 6-Tube Magnetic Separation Rack #7017?
No, these racks cannot be autoclaved. Exposing them to high autoclave temperatures may cause the magnets to lose their magnetism. Instead, we suggest submerging or spraying the ...
Does the CUT&Tag assay show a bias toward euchromatin or heterochromatin?
With the appropriate salt concentration in the tagmention buffer, we don't observe bias toward euchromatin or heterochromatin in our assays. In CUT&Tag, the target-specific...
Where can I find the recommended primary antibody dilution buffer for western blotting (BSA or milk)?
The recommended primary antibody dilution buffer for western blotting (i.e., BSA or nonfat dry milk) can be found either in a grey box on the bottom of the first page of the produc...
Why can't I detect LC3-I?
Our LC3 antibodies provide a good readout for autophagy, as indicated by changes in the abundance of LC3-II. However, our LC3 antibodies may have stronger reactivity with the type ...
What is the concentration of the PTMScan® Ubiquitin Branch Motif (K-ε-GG) Immunoaffinity Beads #1990 component in the PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Kit #5562?
The concentration of the antibody-beads included in the PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Kit #5562 (Bead SKU #1990) is proprietary and therefore we are unable to disclose ...
What is the minimal amount of tissue required for a CUT&RUN experiment?
While we recommend 1mg of tissue per assay in most cases, we have had some success with as little as 0.5 mg of tissue per assay. Easier tissue types, such as liver, or easier targe...
Are you able to disclose the ingredients of the fixative solution in the Senescence β-Galactosidase Staining Kit #9860?
The 10X Fixative Solution found in our Senescence β-Galactosidase Staining Kit #9860 contains 20% formaldehyde and 2% glutaraldehyde in 10X PBS.
Are the Anti-rabbit IgG, HRP-linked Antibody #7074; Anti-mouse IgG, HRP-linked Antibody #7076; and Anti-rat IgG, HRP-linked Antibody #7077 cross-absorbed against other species?
The Anti-rabbit IgG, HRP-linked Antibody #7074 and the Anti-mouse IgG, HRP-linked Antibody #7076 have been cross-adsorbed against human IgG. The Anti-rat IgG, HRP-linked Antibody #...
Does the Cellular Glutathione Detection Assay Kit #13859 detect both reduced and oxidized glutathione?
The Cellular Glutathione Detection Assay Kit #13859 detects only the reduced form of glutathione. It is unable to detect the oxidized form.
I have seen reports that MNase has some sequence bias. Do you see this in your CUT&RUN assays and does it affect the target protein-interaction DNA profiles you get?
No, we do not observe any sequence bias in our CUT&RUN assays. Free MNase may have some sequence bias in the genome, however, in CUT&RUN, the MNase is fused with pAG and re...
How should I search for the Pan-Methyl Lysine PTM in LC-MS/MS data when using your PTMScan® Pan-Methyl Lysine Kit #14809?
Our PTMScan Pan-Methyl Lysine Kit #14809 will enrich for all three forms of methylated lysine: mono, di and tri-methyl lysine. To reduce the complexity of the search space, it is n...
How much AMPKα2 protein is expressed in AMPK Control Cell Extracts #9158?
In-house, we tested AMPK Control Cell Extracts #9158 and mouse liver extract, blotting with AMPKα2 Antibody #2757 (approved for human and monkey) and a competitor AMPKα2 antibody (...
Can I use a BCA assay to check the concentration of my antibody?
A BCA protein assay will likely not provide an accurate concentration for our antibodies formulated in glycerol, due to the presence of BSA and incompatibility with a higher percen...
Will the GFP (D5.1) Rabbit mAb #2956 detect Emerald GFP?
Yes, the GFP (D5.1) Rabbit mAb #2956 will detect Emerald GFP.
Which desalting columns do you recommend to use with the Malachite Green Phosphate Detection Kit #12776 in order to remove contaminating phosphate from the cells?
When developing the Malachite Green Phosphate Detection Kit #12776, we successfully used the Millipore Centrifuge filters AMICON ULTRA 0.5mL, 10,000 NMWL, product # UFC501096.
Will your Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625 also recognize the smaller PARP-1 fragments (40-55 kDa) that are generated during necrosis?
Our Cleaved PARP (Asp214) (D64E10) XP Rabbit mAb #5625 is designed to be specific for the large fragment (89 kDa) of human PARP1 protein that is produced by caspase cleavage at Asp...
Does your Akt (pan) (C67E7) Rabbit mAb #4691 detect PKC?
Our Akt (pan) (C67E7) Rabbit mAb #4691 does not detect over-expressed or endogenous levels of PKC.
What β-Tubulin isoforms and subtypes are detected by CST β-Tubulin antibodies?
Based on homology, our β-Tubulin Antibody #2146, β-Tubulin (9F3) Rabbit mAb #2128, β-Tubulin (D3U1W) Mouse mAb #86298, and β-Tubulin (D2N5G) Rabbit mAb #15115 should detect β-Tubul...
Does each Concanavalin A Magnetic Bead bind to one cell or multiple cells?
The average diameter of each Concanavalin A bead particle is ~1.0µm while the size range of a cell is 0.1-100 µm (the most commonly used cell lines like HeLa, HepG2 and HEK293 all ...
How much of each antibody is supplied in the StemLight™ Pluripotency Antibody Kit #9656 and how does this relate to the number of tests?
There is 50 µl of each antibody component that is listed in the StemLight Pluripotency Antibody Kit #9656. The kit components are pre-optimized for parallel use in immunofluorescen...
Will an antibody recommended for IHC-P with one retrieval buffer work with another?
We have performed antigen retrieval comparisons with a number of products. We consistently see the strongest signals with high pH retrieval buffers. We have not seen the staining...
Why does the observed size versus the theoretical size of TET2 differ by western blot?
TET2 is post-translationally modified (https://www.phosphosite.org/proteinAction.action?id=22594&showAllSites=true). Although the theoretical molecular weight is 223.8 kDa thes...
Why would I use the Fc Block CD16/CD32 (2.4G2) Rat mAb #88280?
Our Fc Block CD16/CD32 (2.4G2) Rat mAb #88280 binds to the Fc receptor on the surface of live cells and blocks the interaction of the Fc receptor with the Fc domain of antibodies. ...
Why do I see two bands for VEGF Receptor 2 by western blot?
Our VEGF Receptor 2 antibodies, i.e., #2472, #2479, and #9698, recognize total VEGFR2, which includes any post-translationally modified versions of the protein. Therefore, because ...
What is the identity of the extra band detected in THP-1 extract by your STING (D2P2F) Rabbit mAb #13647?
We observe multiple bands at or around 33-35 kDa in cells with high STING protein expression, such as THP-1 and HDLM-2. Regrettably, we are not certain about what the lower molecul...
Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) (IF-IC) with human samples?
We have several antibodies for detecting the phosphorylation of Akt at Ser 473 that are validated for IF-IC with human samples. These include the following:
- Phospho-Akt (Se...
How much CUT&RUN DNA is required for NG-seq analysis?
The amount of CUT&RUN DNA required for NG-seq analysis depends on the sensitivity of the DNA library prep kit. The typical yield for CUT&RUN DNA is 0.6 to 6 ng per reaction...
Do I need to perform Fc blocking when using rabbit or mouse antibodies in flow cytometry?
Human Fc-gamma Receptor 1a (FCGR1A) exhibits strong cross-reactivity with rabbit IgG, and mouse IgG2a, IgG2c, and IgG3. Of course, mouse Fc-gamma Receptors will react with mouse an...
What is the fewest number of cells I can use in a reaction with the CST CUT&Tag kit?
We have shown that our CUT&Tag Assay Kit #77552 works with as few as 5,000-10,000 cells for histone modifications and 20,000 cells for transcription factors and cofactors.
Do you have a methylene blue dot blot staining protocol?
We have found that the methylene blue staining of the amounts of DNA spotted when performing a DNA dot blot can be tricky and may need optimization.
1. It is very important ...