Technical Support Articles

Will the Non-phospho (Active) YAP (Ser127) (E6U8Z) Rabbit mAb #29495 cross-react with TAZ?

YAP and TAZ are co-expressed abundantly in every cell line that has been shown to be positive for either protein, and likewise, they are negative/low in the same cell lines (essent...

Can you provide data that confirms the specificity of your BACE (D10E5) Rabbit mAb #5606?

The specificity of our BACE (D10E5) Rabbit mAb #5606 has been confirmed in the literature on brain extracts prepared from BACE1 (-/-) mice. Please see Figure 1 in Shahani, N. et al...

Why don't the phosphorylation sites given in the names of your phospho-EGFR antibodies match the same residue numbers in the UniProt sequence (P00533)?

When EGFR was first reported, the phosphorylation sites and corresponding amino acid numbering did not account for a cleaved signal peptide measuring 24 amino acids in length. Cons...

How long can I leave glycerol on my plates for the Senescence β-Galactosidase Staining Kit #9860?

From our limited in-house testing, we would suggest leaving glycerol on the plates for 3-5 days at most. We have not tested longer time periods than this.

What are the differences between the BrdU Cell Proliferation Chemiluminescent Assay Kit #5492 and the BrdU Cell Proliferation Assay Kit #6813? Which should I choose?

In general, the BrdU Cell Proliferation Chemiluminescent Assay Kit #5492 offers a broader dynamic range and higher sensitivity compared to the BrdU Cell Proliferation Assay Kit #68...

What is your recommended procedure for antigen retrieval with a pressure cooker?

For antigen retrieval in a pressure cooker, bring slides submerged in buffer up to 125C, maintain for 30 seconds, then cool to 90C and maintain that for 10 sec. Remove the containe...

Does the CST CUT&Tag kit work with isolated nuclei?

Our CUT&Tag kit is optimized for use with whole cells and works with a large number of cell lines, both adherent and suspension. While our kit works with pre-isolated nuclei be...

Why should I use the Protein A (HRP Conjugate) #12291 in place of the secondary antibody in my western blot for IP experiments?

Protein A predominantly binds intact IgG and does not bind denatured IgG well. It can be used in IP experiments to help avoid the potential masking of signal by denatured IgG heavy...

What samples and species were tested with the Griess Reagent Nitrite Measurement Kit #13547?

We have tested the Griess Reagent Nitrite Measurement Kit #13547 with media, cell culture supernatant, and urine. It should cross-react with all species types.

Can the Phospho-β-Catenin (Ser33/37) Antibody #2009 bind if the sites are singly phosphorylated or is the phosphorylation at both sites necessary?

We have tested the reactivity of the Phospho-β-Catenin (Ser33/37) Antibody #2009 to Ser33/37 by peptide dot blot. This antibody showed strong signal when phosphorylated at Ser37 al...

Can I use RIPA Buffer (10X) #9806 for ELISA experiments?

Can I use the Cyclic AMP XP® Assay Kit #4339 with tissue samples?

We have not tested tissue samples in-house using the Cyclic AMP XP Assay Kit #4339, so although we cannot guarantee it will work, there is no reason it should not. We recommend loa...

What are the high molecular weight bands detected by the β1-Adrenergic Receptor Antibody #12271?

The antigen for the β1-Adrenergic Receptor Antibody #12271 surrounds Gly406 of the human β1-Adrenergic Receptor protein and is contained entirely in the cytoplasmic domain. #12271 ...

Are the Protein A Magnetic Beads #73778 and the Protein G Magnetic Beads #70024 hydrophilic or hydrophobic?

The base particle of the Protein A Magnetic Beads #73778 and the Protein G Magnetic Beads #70024 is somewhat hydrophilic. It is difficult to predict the hydrophilicity or hydrophob...

Does Cell Lysis Buffer (10X) #9803 work with the Bradford assay?

Cell Lysis Buffer (10X) #9803 will interfere with the Bradford protein assay. This is because it contains detergent. To make the assay somewhat compatible, dilute your protein quan...

What PD-L2 antibodies do you currently offer?

We currently offer three products targeting PD-L2:

Our PD-L2 (D7U8C) XP® Rabbit mAb #82723 recognizes endogenous levels of total human PD-L2 protein and was produced by immu...

Do your PD-1 and PD-L1 antibodies recognize the intracellular or extracellular domain?

The regions recognized by our PD-1 and PD-L1 antibodies are as follows:

PD-1 (EH33) Mouse mAb (IHC-Specific) #43248 binds the extracellular domain.
PD-1 (D7D5W) XP® Rabbi...

If your Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb #6943 detects human Src protein when phosphorylated at Tyr419, why does the product name say Tyr416?

Our Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb #6943 detects endogenous levels of human Src protein only when phosphorylated at Tyr419.  The discrepancy in the product name is ...

Does your Anti-mouse IgG, HRP-linked Antibody #7076 cross react with mouse IgM?

Our Anti-mouse IgG, HRP-linked Antibody #7076 shows approximately 25% reactivity to mouse IgM.

Can I use human PBMCs with Senescence β-Galactosidase Staining Kit #9860?

CST scientists have not tested Senescence β-Galactosidase Staining Kit #9860 using human peripheral blood mononuclear cells (PBMCs). Therefore, we cannot provide a suggested...

Is it possible to perform CUT&Tag on fixed frozen cells?

Yes. Cell fixation is recommended when the cells are very fragile (easily lysed during experiments), when the cellular signaling pathway is sensitive to the Concanavalin A beads...

How much of each antibody is supplied in the StemLight™ Pluripotency Antibody Kit #9656 and how does this relate to the number of tests?

There is 50 µl of each antibody component that is listed in the StemLight Pluripotency Antibody Kit #9656. The kit components are pre-optimized for parallel use in immunofluorescen...

What is the difference between phospho-Smad2 (Ser465/467) and phospho-Smad2 (Ser245/250/255)?

Phosphorylation of serines 465 and 467 indicates Smad2 activation and nuclear localization; whereas, phosphorylation of serines 245, 250, and 255 is inhibitory and leads to retenti...

What is the best way to choose an antibody for my CUT&Tag assay if I can not find a CUT&Tag-validated antibody for my target protein?

We always recommend starting with CST CUT&Tag-validated antibodies because CST scientists perform intensive in-house tests to exclude any antibody that tends to show non-specif...

Does the CUT&Tag assay show a bias toward euchromatin or heterochromatin?

With the appropriate salt concentration in the tagmention buffer, we don't observe bias toward euchromatin or heterochromatin in our assays. In CUT&Tag, the target-specific...

Can I freeze the PTMScan® HS Magnetic Immunoaffinity Beads?

The PTMScan HS Magnetic Immunoaffinity Beads should not be frozen. We recommend storing this product at 4C. However, we have performed functional testing by IAP and subsequent mass...

Which species does the Caspase-3 Activity Assay Kit #5723 work in?

We have validated the Caspase-3 Activity Assay Kit #5723 in human and mouse cell lines. However, as long as there is active caspase-3 present, this kit is predicted to work with mo...

What is the pH of SignalStain® Antibody Diluent #8112?

The pH of our SignalStain^® Antibody Diluent #8112 has a range between 7.38-7.45. 

Do the recombinant SARS-CoV-2 spike proteins from CST have the same folding as the native SARS-CoV-2 spike protein?

Unfortunately, we cannot confirm that the recombinant SARS-CoV-2 spike proteins we provide are in exactly the same conformation that would be expected in nature, as we do not yet h...

Will the RAG1 (D36B3) Rabbit mAb #3968 cross-react with human RAG2?

Our RAG1 (D36B3) Rabbit mAb #3968 was produced using a recombinant protein fragment with a sequence toward the N-terminal region of human RAG1. No significant homology is found in ...