Technical Support Articles
Results (694)
Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?
The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 has been tested succe...
How does the specificity of the F(ab’)2 fragments differ from the corresponding full-length monoclonal antibodies?
What is the enzymatic activity of Micrococcal Nuclease #10011?
Our Micrococcal Nuclease #10011 has an activity level of approximately 1,000,000 gel U/ml (approximately 100,000 Kunitz U/ml, as 10,000 gel units is approximately equal to 1,000 Kunitz units). Therefo...
Which phospho-AMPKα antibody do you suggest for my western blot experiment?
The selection of a phospho-AMPKα antibody depends on the subunit of interest. Cell Signaling Technology has four antibodies that target phospho-AMPKα:
- Phospho-AMPKα (Thr172) (40H9) R...
What is the species reactivity of the SUMO remnant PTMScan® kits that utilize WaLP for peptide digest?
Wild type alpha-lytic protease (WaLP) is a serine endopeptidase that cleaves at the carboxyl terminal side of amino acids alanine, serine, threonine, and valine.
WaLP recognizes and cleaves SUM...
What controls can I use to show my CUT&RUN experiment is working?
Whether performing CUT&RUN with downstream qPCR or NG-seq analysis, we always recommend using a CUT&RUN-validated positive control antibody like our The intended use of the PTMScan® LysC Protease #84748 is to digest proteins into peptides as input for PTMScan Kits where an arginine is part of the PTM motif sequence. While we do not have a general protocol for tissue-based lysate preparation, here are some helpful tips: We have several antibodies for detecting total Akt protein that are validated for IF-IC with human samples. These are as follows:
What is the intended use of PTMScan® LysC Protease #84748?
A list of compatible PTM...How should I prepare my tissue extracts for IP?
Freshly prepared lysates result in fewer nonspecific and degradation bands, and therefore yi...Which total Akt antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
How stable are your glycerol formulated antibodies after freeze/thaw at -80C?
Regrettably, we cannot offer any firm guarantees when glycerol formulated antibodies have been frozen. However, we have performed stability testing by western blot for a representative group of rabbi...
Do you have a protocol for lysing in a 96-well plate (no sonication)?
For lysis in 96-well assay plates, we recommend incubation in the provided lysis buffer for ~30 min at 4°C with gentle shaking. While sonication is difficult in small volumes, we strongly recommend...
What lysis buffer should I use for co-IPs?
We recommend using our Cell Lysis Buffer (10X) #9803 for immunoprecipitation (IP) or co-IP experiments. While both Cell Lysis Buffer(1X) #9803 and RIPA Buffer...
What types of Ras are recognized by your G12D and G12V mutant specific antibodies?
Our Ras (G12D Mutant Specific) (D8H7) Rabbit mAb #14429 and Ras (G12V Mutant Specific) (D2H12) Rabbit mAb #14412 will detect all three types of Ras (H-Ras, K-Ras, and N-Ras) if the respective mutation...
My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?
Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex...
Will the Non-phospho (Active) YAP (Ser127) (E6U8Z) Rabbit mAb #29495 cross-react with TAZ?
YAP and TAZ are co-expressed abundantly in every cell line that has been shown to be positive for either protein, and likewise, they are negative/low in the same cell lines (essentially, they appear t...
Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Mouse” in the first step of the SignalStar Multiplex IHC Panel Builder ...
Can the Lambda Protein Phosphatase Kit #89726 be stored at -20°C?
The Lambda Protein Phosphatase #56206 included in the Lambda Protein Phosphatase Kit #89726 is temperature sensitive. Therefore, we recommend storing the kit at -80°C. However, the kit is stable at -2...
What is the best way to choose an antibody for my CUT&RUN assay if I cannot find a CUT&RUN validated antibody for my target protein?
When selecting an antibody for use in CUT&RUN, we recommend starting with a ChIP or ChIP-seq-validated antibody; however, we have found that not all ChIP-validated antibodies work in the CUT&am...
Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&Tag experiments?
Concanavalin A beads can clump together rather easily, especially when cells become lysed. Make sure your cells are healthy and be sure to treat them very gently during the cell wash. We also recommen...
How long after the completion of staining can I wait to image my slides when performing a SignalStar™ Multiplex IHC assay?
For Imaging Round 1, the staining should show robust signal when imaged up to 8 hours post completion of staining. For Imaging Round 2, imaging should be performed as close to the completion of staini...
Why isn't my bottle of Cell Lysis Buffer (10X) #9803 frozen?
Occasionally, Cell Lysis Buffer (10X) #9803 stored at -20C may not appear frozen, which is not a reason for concern. This phenomenon is known as nucleation, or the initial formation of ice from a liqu...
Is it normal to see blue staining in my untreated samples when using the Senescence β-Galactosidase Staining Kit #9860?
Seeing positive blue staining in your untreated cells is not a cause for concern. We have also observed positive blue staining in our negative controls in some cell types. For example, we always see t...
What is an appropriate positive control to include in this SignalStar™ assay? Are multiple controls necessary?
Any tissue shown to be positive for each marker via chromogenic IHC can serve as a positive control tissue for a SignalStar™ assay. Each target will therefore require a positive control, which may som...
Does the amino acid sequence of SARS-CoV-2 Spike RBD (multimeric) (319-591) Recombinant Protein (8xHis-Tag) #17862 (319-591) being longer than the amino acid sequence of other RBD products (318-541) affect its binding activity with ACE2?
The additional amino acids found in SARS-CoV-2 Spike RBD (multimeric) (319-591) Recombinant Protein (8xHis-Tag) #17862 should not affect the binding of RBD to ACE2 because the RBD region ...
Can my slides be used for other assays after SignalStar™ Multiplex IHC?
SignalStar™ Multiplex IHC Kits & Reagents have not been validated for use in combination with other assays. The SignalStar technology does not destroy the tissue, so it may be possible to perform ...
Can you provide data that confirms the specificity of your BACE (D10E5) Rabbit mAb #5606?
The specificity of our BACE (D10E5) Rabbit mAb #5606 has been confirmed in the literature on brain extracts prepared from BACE1 (-/-) mice. Please see Figure 1 in Shahani, N. et al. (2014 ) J Biol Che...
What DNA library prep kit do you recommend using for the CUT&Tag assay?
We recommend using our CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415 to make DNA libraries for C...
When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. How do I know if this excess staining is correct?
During the course of optimization, we’ve found that fluorescent staining may show higher %-positivity than chromogenic staining. To ensure any excess staining is specific, confirm that the correct sub...
Does the CST CUT&Tag kit work with isolated nuclei?
Our CUT&Tag kit is optimized for use with whole cells and works with a large number of cell lines, both adherent and suspension. While our kit works with pre-isolated nuclei because the Concanaval...
What is the extinction coefficient of my rabbit antibody?
The molar extinction coefficient of rabbit IgG at 280 nm is approximately 210,000 M-1cm-1.