Technical Support Articles
Results (710)
Which phospho-AMPKα antibody do you suggest for my western blot experiment?
The selection of a phospho-AMPKα antibody depends on the subunit of interest. Cell Signaling Technology has four antibodies that target phospho-AMPKα:
- Phospho-AMP...
Are the -20C components within the CUT&RUN Assay Kit #86652 stable to use if they have been stored at 4C for a period of time?
Upon arrival of the kit to your facility, the components should be broken down and stored at their recommended temperatures. We have conducted stability tests for a few components ...
Can the Annexin V-FITC Early Apoptosis Detection Kit #6592 be used on adherent cells?
Our validation of the Annexin V-FITC Early Apoptosis Detection Kit #6592 was performed using live cells in suspension, but the steps can be carried out on live adherent cells as we...
Have species been tested if they are not listed as cross-reactive with a CST antibody on the datasheet?
Possibly. CST scientists test all our antibodies on multiple species when feasible, depending on the target protein and the experimental systems available. If a tested species does...
What isoforms will the WT1 (D6M6S) Rabbit mAb #13580 detect?
Our WT1 (D6M6S) Rabbit mAb #13580 is predicted to detect all 9 isoforms listed in UniProt (UniProt ID - P19544). This includes the isoform containing +KTS.
What advice do you have for collecting samples of adherent cell lines versus suspension cell lines for CUT&Tag experiments?
For CUT&Tag assays using adherent cell lines, the first step is to detach the cells from the dish. We recommend using trypsin to detach the cells. Alternatively, Accutase Cell ...
Which total Akt antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several monoclonal antibodies for detecting total Akt protein that are validated for IHC-P with mouse samples. These are as follows:
- Akt (pan) (C67E7) Rabbit mAb #...
How can I increase the western blot signal for ubiquitin?
The ubiquitin signal can be increased by using a semi-dry-transfer and autoclaving the membrane for 30 minutes on a wet cycle or boiling the membrane for 30 minutes after transfer.
Which p44/42 MAPK (Erk1/2) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) in human samples?
We have several monoclonal antibodies for p44 and p42 mitogen-activated protein kinases (MAPKs), also referred to as Erk2 and Erk1, that are validated for IF-IC with human samples....
How should I resuspend and aliquot the PTMScan® LysC Protease #84748?
To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, ...
Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Human” in the first step of the SignalStar Multiplex IH...
Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?
The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:
PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure...
What is the composition of the DNA Elution Buffer #10009?
The composition of our DNA Elution Buffer #10009 is 10 mM Tris-Cl, pH 8.5.
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic deterg...
How do I avoid batch effects and what sample run order should I use for PTMScan® experiments?
To avoid intra-sample batch effects during liquid chromatography with tandem mass spectrometry (LC-MS/MS), we recommend a run order that involves running one technical replicate of...
Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??
Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&a...
Is there a maximum number of cells that can be used in a single CUT&Tag assay?
We have found 100,000 cells per assay to be sufficient for enriching target loci for multiple histone modifications, transcription factors, and transcription cofactors. However, on...
How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?
We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reage...
Can I use the Cell Fractionation Kit #9038 with tissue samples?
Since the Cell Fractionation Kit #9038 is primarily meant to be used for cultured cell lines, the buffers have not been optimized for use with tissue samples. Therefore, the fracti...
Can I modify the CST-recommended protocol for a given application?
CST antibodies undergo thorough validation processes to ensure reliable performance for every approved application. This includes testing different protocol variations to identify ...