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International Units (IU) and specific activity are two differe...
The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure...
To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, ...
Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic deterg...
Yes, please select “Human” in the first step of the SignalStar Multiplex IH...
CST antibodies undergo thorough validation processes to ensure reliable performance for every approved application. This includes testing different protocol variations to identify ...
We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reage...
CST thoroughly validates each antibody available in the Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using? The SignalStar Multiplex IHC kits and reagents have been optimized with respect to fluorophore pairing and order of antibodies. As tissues vary in quality and expression level of b... Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552?? Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&a... Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species? The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 h... How do the Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 and the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 compare in terms of their sensitivity and specificity? The Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 is a mouse mAb from a single clone, whereas the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 is a mix of rabbit mAb... Can I reuse PTMScan® antibody beads? The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic e... Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples? We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include: β-Actin (13E5) Rabbit mAb #4970β-Actin...
The SignalStar Multiplex IHC kits and reagents have been optimized with respect to fluorophore pairing and order of antibodies. As tissues vary in quality and expression level of b...
Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&a...
The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 h...
The Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 is a mouse mAb from a single clone, whereas the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 is a mix of rabbit mAb...
The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic e...
We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include:
The Concanavalin A beads can clump together rather easily, especially when the cells become lysed. To avoid this, make sure your cells are healthy and be sure to treat them very ge...
We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with human samples. These are:
We have several antibodies for detecting Akt phosphorylation at Ser473 that are validated for IHC-P with human samples. These are:
No, these racks cannot be autoclaved. Exposing them to high autoclave temperatures may cause the magnets to lose their magnetism. Instead, we suggest submerging or spraying the ...
For mouse IgGs, you will need to determine the isotype of the primary antibody and use the appropriate isotype control:IgG1: Mouse (G3A1) mAb IgG1 Isotype Control #5415IgG2...
For CUT&Tag assays using adherent cell lines, the first step is to detach the cells from the dish. We recommend using trypsin to detach the cells. Alternatively, Accutase Cell ...
Yes. We have shown that our CUT&Tag Assay Kit #77552 works with mouse liver, mouse brain, and mouse heart tissues for histone targets with as little as 1 mg of tissue for each ...
The digestion is performed at room temperature to minimize carbamylation (also known as carbamoylation) of the sample peptides in the urea buffer, which can interfere with downstre...
The most sensitive antibody will depend on whether you are trying to detect Cas9 protein from Streptococcus pyogenes, Staphylococcus aureus, or Campylobacter jejun...
For the 96-well colorimetric PathScan® and FastScan® ELISA plates, the specification information can be found in the How were the sequences of the PTMScan® Control peptides chosen? The sequences of the synthetic peptides were based on modified peptides that we have consistently recovered and identified in human and mouse sam... Which method is better for purifying CUT&Tag DNA, DNA affinity columns or a phenol-chloroform DNA extraction using ethanol precipitation? We recommend using DNA Purification Buffers and Spin Columns (ChIP, CUT&RUN, CUT&Tag) #14209 when purifying your CUT&Tag DNA. Although phenol-chloroform extraction foll... Which cleaved caspase-3 (Asp175) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples? We have several antibodies for detecting Cleaved Caspase-3 (Asp175) that are validated for IF-IC with human samples. These are: Cleaved Caspase-3 (Asp175) Antibody #9661 Do your DYKDDDDK Tag antibodies detect 3X FLAG tag? Yes, our DYKDDDDK Tag antibodies will detect 3X FLAG tag as long as the FLAG tag sequence is located on the N-terminal or C-terminal end. Why is the concentration of my antibody so low? At CST, our antibodies are formulated on the basis of their reactivity and performance for each approved application at a recommended dilution. For antibodies with high affinit...
The sequences of the synthetic peptides were based on modified peptides that we have consistently recovered and identified in human and mouse sam...
We recommend using DNA Purification Buffers and Spin Columns (ChIP, CUT&RUN, CUT&Tag) #14209 when purifying your CUT&Tag DNA. Although phenol-chloroform extraction foll...
We have several antibodies for detecting Cleaved Caspase-3 (Asp175) that are validated for IF-IC with human samples. These are:
Yes, our DYKDDDDK Tag antibodies will detect 3X FLAG tag as long as the FLAG tag sequence is located on the N-terminal or C-terminal end.
At CST, our antibodies are formulated on the basis of their reactivity and performance for each approved application at a recommended dilution. For antibodies with high affinit...
