Technical Support Articles
Results (713)
What is the best way to choose an antibody for my CUT&RUN assay if I cannot find a CUT&RUN validated antibody for my target protein?
When selecting an antibody for use in CUT&RUN, we recommend starting with a ChIP or ChIP-seq-validated antibody; however, we have found that not all ChIP-validated antibodie...
How long after the completion of staining can I wait to image my slides when performing a SignalStar™ Multiplex IHC assay?
For Imaging Round 1, the staining should show robust signal when imaged up to 8 hours post completion of staining. For Imaging Round 2, imaging should be performed as close to the ...
Can I autoclave the 6-Tube Magnetic Separation Rack #7017?
No, these racks cannot be autoclaved. Exposing them to high autoclave temperatures may cause the magnets to lose their magnetism. Instead, we suggest submerging or spraying the ...
Which caspase-3 antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have two antibodies for detecting caspase-3 that are validated for IHC-P with human samples, Caspase-3 Antibody #9662 and Caspase-3 (D3R6Y) Rabbit mAb (IHC Formulated) #14214. W...
How should I store my antibody?
Proper storage of your antibody may vary depending on the product's storage buffer and antibody conjugation. Therefore, please refer to the "Storage" section of the p...
Which total Akt antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) with mouse samples?
We have several total Akt antibodies that are validated for IF-IC with mouse samples. These are:
- Akt (pan) (C67E7) Rabbit mAb #4691
- Akt (pan) (11E7) Rabbit mAb #468...
How much CUT&RUN DNA is required for NG-seq analysis?
The amount of CUT&RUN DNA required for NG-seq analysis depends on the sensitivity of the DNA library prep kit. The typical yield for CUT&RUN DNA is 0.6 to 6 ng per reaction...
What are the specifications for the 96-well plate in the CST ELISA kits?
For the 96-well colorimetric PathScan® and FastScan® ELISA plates, the specification information can be found in the We have several monoclonal antibodies for detecting total Akt protein that are validated for IHC-P in human samples. These are as follows:
Which total Akt antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
Which phospho-S6 ribosomal protein (Ser235/236) antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
We have several antibodies for detecting the phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 that are validated for IF-IC with human samples. These are as follows:
Do your DYKDDDDK Tag antibodies detect 3X FLAG tag?
Yes, our DYKDDDDK Tag antibodies will detect 3X FLAG tag as long as the FLAG tag sequence is located on the N-terminal or C-terminal end.
Does your Cas9 (7A9-3A3) Mouse mAb #14697 detect the D10A/H840A mutant (dCas9) and the D10A mutant (niCas9)?
Our Cas9 (7A9-3A3) Mouse mAb #14697 was produced with a recombinant protein specific to the amino terminus of Cas9 from Streptococcus pyogenes (UniProt ID #Q99ZW2; https://w...
Are Smad8 and Smad9 the same protein?
Smad8 and Smad9 are often used interchangeably in the literature to refer to the same protein. However, the official name of the protein is Smad9. Moreover, there ...
Will your Anti-biotin, HRP-linked Antibody #7075 cross-react with endogenous biotin in cell lysates?
Our Anti-biotin, HRP-linked Antibody #7075 is intended for use with our Biotinylated Protein Ladder Detection Pack #7727. However, it is possible for this antibody to also detec...
Which cleaved PARP antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have several antibodies for cleaved PARP that are validated for IHC-P in human samples. These are SignalStar Multiplex IHC kits and reagents haven’t yet been validated for use with antibodies outside of our menu. We’re in the proces... The suggested protocol for treating cells with poly(dA:dT) can be found below: With the appropriate salt concentration in the tagmention buffer, we don't observe bias toward euchromatin or heterochromatin in our assays. In CUT&Tag, the target-specific... We have several antibodies for detecting the phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 that are validated for IHC-P with human samples. These are S6 Ribosomal Pr... The recommended primary antibody dilution buffer for western blotting (i.e., BSA or nonfat dry milk) can be found either in a grey box on the bottom of the first page of the produc... We are currently unable to provide the Solution B component separately from the Senescence β-Galactosidase Staining Kit #9860. However, we can disclose the ingredients of Solution ... Since the Cell Fractionation Kit #9038 is primarily meant to be used for cultured cell lines, the buffers have not been optimized for use with tissue samples. Therefore, the fracti... Yes, please select “Mouse” in the first step of the SignalStar Multiplex IH... Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are st... During the course of optimization, we’ve found that fluorescent staining may show higher %-positivity than chromogenic staining. To ensure any excess staining is specific, confirm ... Any tissue shown to be positive for each marker via chromogenic IHC can serve as a positive control tissue for a SignalStar™ assay. Each target will therefore require a positive co... SignalStar Multiplex IHC kits and reagents have been validated for use in combination with direct conjugates. The SignalStar™ Fluorescence Removal Kit PTMScan® is compatible with the use of stable isotope labeling using amino acids in cell culture, or SILAC. The incorporation of heavy stable iso... We have several antibodies for detecting the phosphorylation of Akt at Ser 473 that are validated for IF-IC with human samples. These include the following:
The molar extinction coefficient of rabbit IgG at 280 nm is approximately 210,000 M-1cm-1.
I don’t see my target of interest in your menu of available antibodies for SignalStar™ Multiplex IHC. Can I still use it in my panel in some way?
Does CST have a protocol for treating cells with poly(dA:dT)?
Poly(dA:dT) Treatment Protocol (5 μg/mL final concentra...
Does the CUT&Tag assay show a bias toward euchromatin or heterochromatin?
Which S6 ribosomal protein antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
Where can I find the recommended primary antibody dilution buffer for western blotting (BSA or milk)?
Can I purchase Solution B outside of the Senescence β-Galactosidase Staining Kit #9860?
Can I use the Cell Fractionation Kit #9038 with tissue samples?
Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?
My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?
When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. How do I know if this excess staining is correct?
What is an appropriate positive control to include in this SignalStar™ assay? Are multiple controls necessary?
Can I combine antibodies used in a SignalStar™ assay with direct conjugates?
Is PTMScan® compatible with SILAC?
Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) (IF-IC) with human samples?
What is the extinction coefficient of my rabbit antibody?