Technical Support Articles
Results (694)
How do I QC my CUT&RUN DNA before spending money on NG-seq?
You can QC your CUT&RUN assay prior to NG-seq by performing qPCR analysis of your enriched DNA. Our First, ensure blood is collected in a vacutainer tube that does not contain any anticoagulants such as sodium heparin or EDTA. We do not observe any bias toward euchromatin or heterochromatin in our assays. In CUT&RUN, the target-specific antibody recruits the pAG-MNase and directs digestion to chromatin that is directly ... Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77... We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include: What protocol should I use to prepare serum lysates for western blot?
1. In a biosafety hood transfer the blood into an appropriately sized ...Does CUT&RUN show a bias toward euchromatin or heterochromatin?
Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??
Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?
Is it possible to perform CUT&Tag on frozen tissue samples?
Yes, our CUT&Tag Assay Kit works with flash frozen tissue samples or tissue samples directly frozen at -80℃. After thawing, fixation of the tissue sample is optional, as we haven't found that ...
Can I store the unopened, lyophilized PTMScan® LysC Protease #84748 at -80°C instead of -20°C?
Yes, the lyophilized PTMScan® LysC Protease #84748 may be stored at -80°C. However, we do not recommend storing the LysC powder in a frost-free freezer or a freezer that undergoes freeze-thaw cycles. ...
Which total Akt antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
We have several antibodies for detecting total Akt protein that are validated for IF-IC with human samples. These are as follows:
- Akt (pan) (C67E7) Rabbit mAb #4691
- Akt (pan) (11E7) Ra...
Why do my Concanavalin A Magnetic Beads #82307 appear aggregated?
As long as your Concanavalin A Magnetic Beads #82307 have been stored at the appropriate recommended temperature, the appearance of aggregation is not a cause for concern. By nature, the bead particle...
Can I order a custom formulation of an antibody?
For custom (carrier-free) orders, many antibodies are available pre-formulated in PBS without BSA or glycerol. This carrier-free formulation is amenable to conjugation and can be conjugated to fluorop...
Does this antibody work for my application (western blot, immunoprecipitation, immunofluorescence, immunohistochemistry, ELISA, flow cytometry, chromatin immunoprecipitation, etc.)?
CST routinely tests antibodies in relevant applications. Those that pass rigorous application-specific validation standards are recommended. We only recommend applications that have passed rigorous va...
My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?
Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex...
What is the solution formulation of the PTMScan Control peptides?
The solution formulation of the PTMScan Control peptides is 25% acetonitrile, 74.9% water, 0.1% trifluoroacetic acid (V:V).
Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Mouse” in the first step of the SignalStar Multiplex IHC Panel Builder ...
What is the enzymatic activity of Micrococcal Nuclease #10011?
Our Micrococcal Nuclease #10011 has an activity level of approximately 1,000,000 gel U/ml (approximately 100,000 Kunitz U/ml, as 10,000 gel units is approximately equal to 1,000 Kunitz units). Therefo...
What is the best way to choose an antibody for my CUT&RUN assay if I cannot find a CUT&RUN validated antibody for my target protein?
When selecting an antibody for use in CUT&RUN, we recommend starting with a ChIP or ChIP-seq-validated antibody; however, we have found that not all ChIP-validated antibodies work in the CUT&am...
Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) with mouse samples?
We have several antibodies for detecting Akt phosphorylation at Ser473 that are validated for IF-IC with mouse tissues. These are:
- Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060
How long after the completion of staining can I wait to image my slides when performing a SignalStar™ Multiplex IHC assay?
For Imaging Round 1, the staining should show robust signal when imaged up to 8 hours post completion of staining. For Imaging Round 2, imaging should be performed as close to the completion of staini...
What controls can I use to show my CUT&RUN experiment is working?
Whether performing CUT&RUN with downstream qPCR or NG-seq analysis, we always recommend using a CUT&RUN-validated positive control antibody like our ZBP1 (Z-DNA binding protein 1), also referred to as DAI (DNA-dependent activator of IFN-regulatory factors) and DLM-1, is a nucleotide-binding protein that plays a role in tumorigenesis and innate imm... Any tissue shown to be positive for each marker via chromogenic IHC can serve as a positive control tissue for a SignalStar™ assay. Each target will therefore require a positive control, which may som... We have several antibodies for detecting Akt phosphorylation at Ser473 that are validated for IHC-P with human samples. These are:
What isoforms does the ZBP1 Antibody #60968 recognize?
What is an appropriate positive control to include in this SignalStar™ assay? Are multiple controls necessary?
Which phospho-Akt (Ser473) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
Can I combine antibodies used in a SignalStar™ assay with direct conjugates?
SignalStar Multiplex IHC kits and reagents have been validated for use in combination with direct conjugates. The SignalStar™ Fluorescence Removal Kit To confirm digestion, we recommend taking a ~1% aliquot of the protein sample pre- and post-digestion and running them on an SDS-PAGE gel with Coomassie blue staining. After digestion, you should ... During the course of optimization, we’ve found that fluorescent staining may show higher %-positivity than chromogenic staining. To ensure any excess staining is specific, confirm that the correct sub... Although LysC can digest proteins at high urea concentrations (~8M), we recommend diluted urea (~2M) to minimize the risk of carbamylation. The intended use of the PTMScan® LysC Protease #84748 is to digest proteins into peptides as input for PTMScan Kits where an arginine is part of the PTM motif sequence. No, these racks cannot be autoclaved. Exposing them to high autoclave temperatures may cause the magnets to lose their magnetism. Instead, we suggest submerging or spraying the rack with 10% bleach... Product Iba1/AIF-1 (E4O4W) XP® Rabbit mAb #17198 is predicted to detect isoform 1 and isoform 2 of the Ionized calcium-binding adaptor molecule 1 (Iba1), also known as allograft inflammator... We have several antibodies for detecting the phosphorylation of Akt at Ser 473 that are validated for IF-IC with human samples. These include the following:
How can I confirm the PTMScan® LysC Protease #84748 digested my proteins efficiently?
When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. How do I know if this excess staining is correct?
Why is the PTMScan® LysC Protease #84748 digestion performed in a diluted urea formulation?
What is the intended use of PTMScan® LysC Protease #84748?
A list of compatible PTM...Can I autoclave the 6-Tube Magnetic Separation Rack #7017?
Which isoforms does the ba1/AIF-1 (E4O4W) XP® Rabbit mAb #17198 detect?
Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) (IF-IC) with human samples?