Technical Support Articles
Results (710)
Can I order a custom formulation of an antibody?
For custom (carrier-free) orders, many antibodies are available pre-formulated in PBS without BSA or glycerol. This carrier-free formulation is amenable to conjugation and can be c...
What is the solution formulation of the PTMScan Control peptides?
The solution formulation of the PTMScan Control peptides is 25% acetonitrile, 74.9% water, 0.1% trifluoroacetic acid (V:V).<...
Why do my Concanavalin A Magnetic Beads #82307 appear aggregated?
As long as your Concanavalin A Magnetic Beads #82307 have been stored at the appropriate recommended temperature, the appearance of aggregation is not a cause for concern. By natur...
Why is a fluorescent conjugate of a rabbit antibody giving no signal on compensation beads in flow cytometry?
If you are using compensation beads in your flow cytometry experiment and observe no signal with one of our fluorescent conjugates of a rabbit antibody, this may be due to the reac...
Which S6 ribosomal protein antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
We have several monoclonal antibodies for detecting ribosomal protein S6 that are validated for IF-IC with human samples. These are S6 Ribosomal Protein (5G10) Rabbit mAb #2217 ...
Is it possible to perform CUT&Tag on samples like heart, brain, or developing mouse tissues?
Yes. We have shown that our CUT&Tag Assay Kit #77552 works with mouse liver, mouse brain, and mouse heart tissues for histone targets with as little as 1 mg of tissue for each ...
Does CST have a protocol for treating cells with poly(dA:dT)?
The suggested protocol for treating cells with poly(dA:dT) can be found below:
Poly(dA:dT) Treatment Protocol (5 μg/mL final concentra...
What secondary antibodies do you recommend for fluorescent western blotting?
We currently have four different secondary antibodies approved for use in fluorescent western blotting, depending on species and wavelength of detection:
- Anti-mouse IgG (H+...
Can I use the Cell Fractionation Kit #9038 with tissue samples?
Since the Cell Fractionation Kit #9038 is primarily meant to be used for cultured cell lines, the buffers have not been optimized for use with tissue samples. Therefore, the fracti...
Why are the beads from PTMScan® HS Kits aggregating against the sides of the tube?
Through in-house testing, we have found that the magnetic beads from our PTMScan HS kits aggregate and stick to the walls of some specific brands of tubes. Moreover, they cannot be...
How do you make the 4X RNA Denaturing Buffer for RNA dot blot?
4X RNA Denaturing Buffer Recipe for use with our RNA dot blot protocol:
657ul Formamide @99.5%
210ul of 37% Formaldehyde
133ul of 10x MOPS buffer
Final formulat...
Which phospho-S6 ribosomal protein (Ser235/236) antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) with mouse samples?
We have several antibodies for detecting the phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 that are validated for IF-IC with mouse samples. These are Phospho-S6 Ribo...
Can I use mixed species samples for a PTMScan® experiment?
The PTMScan® technology is compatible with samples derived from all kingdoms of life as well as samples containing mixed species like xenografts (mouse and human), microbiome sampl...
Which Cadherins does the Pan-Cadherin Antibody #4068 detect?
The Pan-Cadherin Antibody #4068 can detect R-, N-, E-, and VE-Cadherin. It does not detect P- or K-Cadherin. Reactivity to other Cadherins has not been tested.
Does the Cellular Glutathione Detection Assay Kit #13859 detect both reduced and oxidized glutathione?
The Cellular Glutathione Detection Assay Kit #13859 detects only the reduced form of glutathione. It is unable to detect the oxidized form.
How much sample is required for a PTMScan® experiment?
We recommend using 10 mg of protein per immunoaffinity purification (IAP) sample for our standard PTMScan kits. For the PTMScan HS product line, 1 mg of starting material is optima...
Why does the observed size versus the theoretical size of TET2 differ by western blot?
TET2 is post-translationally modified (https://www.phosphosite.org/proteinAction.action?id=22594&showAllSites=true). Although the theoretical molecular weight is 223.8 kDa thes...
Have you done any cross-reactivity testing with α-CGRP, β-CGRP and unprocessed precursors using the CGRP (D5R8F) Rabbit mAb #14959?
Our CGRP (D5R8F) Rabbit mAb #14959 is produced with a synthetic peptide corresponding to residues surrounding Val114 of human CGRP protein (UniProt ID P06881 isoform 3; http://www....
What β-Tubulin isoforms and subtypes are detected by CST β-Tubulin antibodies?
Based on homology, our β-Tubulin Antibody #2146, β-Tubulin (9F3) Rabbit mAb #2128, β-Tubulin (D3U1W) Mouse mAb #86298, and β-Tubulin (D2N5G) Rabbit mAb #15115 should detect β-Tubul...
Will an antibody recommended for IHC-P with one retrieval buffer work with another?
We have performed antigen retrieval comparisons with a number of products. We consistently see the strongest signals with high pH retrieval buffers. We have not seen the staining...
How do I lyse my cells for the Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) #23833?
Can I use the Cyclic AMP XP® Assay Kit #4339 with tissue samples?
We have not tested tissue samples in-house using the Cyclic AMP XP Assay Kit #4339, so although we cannot guarantee it will work, there is no reason it should not. We recommend loa...
Does each Concanavalin A Magnetic Bead bind to one cell or multiple cells?
The average diameter of each Concanavalin A bead particle is ~1.0µm while the size range of a cell is 0.1-100 µm (the most commonly used cell lines like HeLa, HepG2 and HEK293 all ...
Why should I use the Protein A (HRP Conjugate) #12291 in place of the secondary antibody in my western blot for IP experiments?
Protein A predominantly binds intact IgG and does not bind denatured IgG well. It can be used in IP experiments to help avoid the potential masking of signal by denatured IgG heavy...
What is the identity of the extra band detected in THP-1 extract by your STING (D2P2F) Rabbit mAb #13647?
We observe multiple bands at or around 33-35 kDa in cells with high STING protein expression, such as THP-1 and HDLM-2. Regrettably, we are not certain about what the lower molecul...
What are the three bands detected by western blot with your VHL Antibody #68547?
Please see Maniaci, C. et al. (2017) Nat. Commun. 8, 830 (PMID: 29018234; https://pubmed.ncbi.nlm.nih.gov/29018234/) and Frost, J. et al. (2021) J. Biol. Chem. 297, 100910 (PMID: 3...
What is the best way to choose an antibody for my CUT&Tag assay if I can not find a CUT&Tag-validated antibody for my target protein?
We always recommend starting with CST CUT&Tag-validated antibodies because CST scientists perform intensive in-house tests to exclude any antibody that tends to show non-specif...
To store plates long-term when using the Senescence β-Galactosidase Staining Kit #9860, do I need to wash off the β-Galactosidase staining solution prior to overlaying with 70% glycerol?
In section C, part 8 of the Senescence β-Galactosidase Staining Kit #9860 protocol, you may overlay the plates with 70% glycerol directly after removing the staining solution. It i...
Why doesn't PD-L2 migrate at 31 kDa?
The predicted molecular weight of PD-L2 is 31 kDa (based on amino acid composition). However, in most models, PD-L2 migrates at an observed molecular weight of 45-60 kDa on Tris-Gl...
How long can I leave glycerol on my plates for the Senescence β-Galactosidase Staining Kit #9860?
From our limited in-house testing, we would suggest leaving glycerol on the plates for 3-5 days at most. We have not tested longer time periods than this.