Technical Support Articles
Results (711)
How are the SignalStar™ Multiplex IHC Kits & Reagents validated?
CST thoroughly validates each antibody available in the We do not observe any bias toward euchromatin or heterochromatin in our assays. In CUT&RUN, the target-specific antibody recruits the pAG-MNase and directs digestion to chromat... ZBP1 (Z-DNA binding protein 1), also referred to as DAI (DNA-dependent activator of IFN-regulatory factors) and DLM-1, is a nucleotide-binding protein that plays a role in tumorige... We recommend using a CUT&Tag-validated positive control antibody, like our Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751, in your experiment to confirm that your CUT&am... For lysis in 96-well assay plates, we recommend incubation in the provided lysis buffer for ~30 min at 4°C with gentle shaking. While sonication is difficult in small volumes, w... Product Iba1/AIF-1 (E4O4W) XP® Rabbit mAb #17198 is predicted to detect isoform 1 and isoform 2 of the Ionized calcium-binding adaptor molecule 1 (Iba1), also known as a... We have several monoclonal antibodies studying p44 and p42 mitogen-activated protein kinases (MAPKs), also referred to as Erk2 and Erk1, that are validated for IHC-P for mouse samp... We have found 100,000 cells per assay to be sufficient for enriching target loci for multiple histone modifications, transcription factors, and transcription cofactors. However, on... To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, ... Yes, please select “Human” in the first step of the SignalStar Multiplex IH... The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers: Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic deterg... CST antibodies undergo thorough validation processes to ensure reliable performance for every approved application. This includes testing different protocol variations to identify ... We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reage... These synthetic peptides are recoverable using our immunoaffinity purification (IAP) antibody beads, and thus provide users with a measure of PTM... Whether performing CUT&RUN with downstream qPCR or NG-seq analysis, we always recommend using a CUT&RUN-validated positive control antibody like our In addition to a known positive control sample, we recommend the following negative controls for your Simple Western™ experiment. We have several antibodies for detecting Akt phosphorylation at Ser473 that are validated for IHC-P with human samples. These are:
During our extensive validation process, we found that some targets do not perform optimally with specific fluorophore pairings. When this is the case, the SignalStar Multiplex IHC... We have several antibodies for detecting Akt phosphorylation at Ser473 that are validated for IF-IC with mouse tissues. These are: The intended use of the PTMScan® LysC Protease #84748 is to digest proteins into peptides as input for PTMScan Kits where an arginine is part of the PTM motif sequence. We have several antibodies for detecting total Akt protein that are validated for IF-IC with human samples. These are as follows:
To confirm digestion, we recommend taking a ~1% aliquot of the protein sample pre- and post-digestion and running them on an SDS-PAGE gel with Coomassie blue staining. After di... Although the lyophilized powder can be stored at -20°C, we recommend long-term storage of the resuspended LysC at -80°C. We do not recommend long-term storage of the resuspended Ly... Yes, the lyophilized PTMScan® LysC Protease #84748 may be stored at -80°C. However, we do not recommend storing the LysC powder in a frost-free freezer or a freezer that undergoes ... Although LysC can digest proteins at high urea concentrations (~8M), we recommend diluted urea (~2M) to minimize the risk of carbamylation. Yes, please select “Mouse” in the first step of the SignalStar Multiplex IH... The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 h... Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are st...
Does CUT&RUN show a bias toward euchromatin or heterochromatin?
Is Fc blocking needed for F(ab') antibody fragments used in live cell flow cytometry assays?
What isoforms does the ZBP1 Antibody #60968 recognize?
What positive and negative controls can I use to demonstrate that my CUT&Tag experiment is successful?
Do you have a protocol for lysing in a 96-well plate (no sonication)?
Which isoforms does the ba1/AIF-1 (E4O4W) XP® Rabbit mAb #17198 detect?
Which p44/42 MAPK (Erk1/2) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
Is there a maximum number of cells that can be used in a single CUT&RUN assay?
How should I resuspend and aliquot the PTMScan® LysC Protease #84748?
Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?
Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?
PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure...
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Can I modify the CST-recommended protocol for a given application?
How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?
What is the intended use of the PTMScan® Control peptides?
What controls can I use to show my CUT&RUN experiment is working?
What controls should I include when performing a Simple Western™ experiment?
1. A no lysate control. This includes the p...
Which phospho-Akt (Ser473) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
Why can't I add a target to the channel I want when using the SignalStar™ Multiplex IHC Panel Builder?
Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) with mouse samples?
What is the intended use of PTMScan® LysC Protease #84748?
A li...
Which total Akt antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
How can I confirm the PTMScan® LysC Protease #84748 digested my proteins efficiently?
Can I store the resuspended PTMScan® LysC Protease #84748 aliquots at -20°C instead of -80°C?
Can I store the unopened, lyophilized PTMScan® LysC Protease #84748 at -80°C instead of -20°C?
Why is the PTMScan® LysC Protease #84748 digestion performed in a diluted urea formulation?
Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?
Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?
My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?