Technical Support Articles
Results (710)
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic deterg...
Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Human” in the first step of the SignalStar Multiplex IH...
How should I resuspend and aliquot the PTMScan® LysC Protease #84748?
To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, ...
Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?
The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:
PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure...
What is the percent cross-reactivity of Anti-mouse IgG, HRP-linked Antibody #7076 with rat and human IgG and IgM?
The percent cross-reactivity of Anti-mouse IgG, HRP-linked Antibody #7076 is 6% for rat IgG and <1.0% for rat IgM, human IgG, and human IgM.
What is the intended use of the PTMScan® Control peptides?
These synthetic peptides are recoverable using our immunoaffinity purification (IAP) antibody beads, and thus provide users with a measure of PTM...
Why do my Concanavalin A Magnetic Beads #82307 appear aggregated?
As long as your Concanavalin A Magnetic Beads #82307 have been stored at the appropriate recommended temperature, the appearance of aggregation is not a cause for concern. By natur...
Does this antibody work for my application (western blot, immunoprecipitation, immunofluorescence, immunohistochemistry, ELISA, flow cytometry, chromatin immunoprecipitation, etc.)?
CST routinely tests antibodies in relevant applications. Those that pass rigorous application-specific validation standards are recommended. We only recommend applications that hav...
Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??
Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&a...
Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?
The SignalStar Multiplex IHC kits and reagents have been optimized with respect to fluorophore pairing and order of antibodies. As tissues vary in quality and expression level of b...
My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?
Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are st...
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with mouse samples. These are:
- Cleaved Caspase-3 (Asp175) Antibody #9...
Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Mouse” in the first step of the SignalStar Multiplex IH...
Is the “Best By” date the expiration date for the product? Will the product still work after that date?
Our products can be expected to perform at optimal levels up until the printed "Best" by date on the vial when stored under the recommended conditions. Many of our produc...
I don’t see my target of interest in your menu of available antibodies for SignalStar™ Multiplex IHC. Can I still use it in my panel in some way?
SignalStar Multiplex IHC kits and reagents haven’t yet been validated for use with antibodies outside of our menu. We’re in the proces...
How can I confirm the PTMScan® LysC Protease #84748 digested my proteins efficiently?
To confirm digestion, we recommend taking a ~1% aliquot of the protein sample pre- and post-digestion and running them on an SDS-PAGE gel with Coomassie blue staining. After di...
How are TrkA, TrkB, and TrkC activated?
The family of Trk receptor tyrosine kinases consists of TrkA, TrkB, and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurot...
Can I store the unopened, lyophilized PTMScan® LysC Protease #84748 at -80°C instead of -20°C?
Yes, the lyophilized PTMScan® LysC Protease #84748 may be stored at -80°C. However, we do not recommend storing the LysC powder in a frost-free freezer or a freezer that undergoes ...
Will your beta-amyloid antibodies detect the alpha-oligomer of the amyloid-beta protein?
Unfortunately, we are not aware if any of our amyloid-beta antibodies will detect the alpha-oligomer of the amyloid-beta protein.
Which total Akt antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
We have several antibodies for detecting total Akt protein that are validated for IF-IC with human samples. These are as follows:
- Akt (pan) (C67E7) Rabbit mAb #4691
Is PTMScan® compatible with SILAC?
PTMScan® is compatible with the use of stable isotope labeling using amino acids in cell culture, or SILAC. The incorporation of heavy stable iso...
Can I store the resuspended PTMScan® LysC Protease #84748 aliquots at -20°C instead of -80°C?
Although the lyophilized powder can be stored at -20°C, we recommend long-term storage of the resuspended LysC at -80°C. We do not recommend long-term storage of the resuspended Ly...
Which nuclear dyes can be used for In-Cell Western assays in combination with DyLight 680-conjugated secondary antibodies?
When performing In-Cell Western assays with DyLight 680-conjugated secondary antibodies, we recommend using either Hoechst 33342 or DAPI as a nuclear dye. DRAQ5 cannot be used beca...
Why is the PTMScan® LysC Protease #84748 digestion performed in a diluted urea formulation?
Although LysC can digest proteins at high urea concentrations (~8M), we recommend diluted urea (~2M) to minimize the risk of carbamylation.
How can I place an order using a quote?
For our US-based customers, quotes may be used on orders placed by phone at 877-616-2355 or by email at orders@cellsignal....
Why do I see a doublet band when probing with Rpb1 NTD (D8L4Y) Rabbit mAb #14958 for Western blot?
The two bands detected by our Rpb1 NTD (D8L4Y) Rabbit mAb (#14958) represent hyperphosphorylated (upper) and non-phosphorylated/hypophosphorylated (lower) Rpb1 (see PMID: 185888...
What is the solution formulation of the PTMScan Control peptides?
The solution formulation of the PTMScan Control peptides is 25% acetonitrile, 74.9% water, 0.1% trifluoroacetic acid (V:V).<...
Which total Akt antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several monoclonal antibodies for detecting total Akt protein that are validated for IHC-P with mouse samples. These are as follows:
- Akt (pan) (C67E7) Rabbit mAb #...
What are the specifications for the 96-well plate in the CST ELISA kits?
For the 96-well colorimetric PathScan® and FastScan® ELISA plates, the specification information can be found in the We have several antibodies for detecting Akt phosphorylation at Ser473 that are validated for IF-IC with mouse tissues. These are:
Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) with mouse samples?