Technical Support Articles
Results (695)
Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Human” in the first step of the SignalStar Multiplex IHC Panel Builder ...
Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?
The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:
PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2B
PMID: 249...
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p...
Is there a maximum number of cells that can be used in a single CUT&Tag assay?
We have found 100,000 cells per assay to be sufficient for enriching target loci for multiple histone modifications, transcription factors, and transcription cofactors. However, one can increase the n...
Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??
Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77...
Will your Anti-biotin, HRP-linked Antibody #7075 cross-react with endogenous biotin in cell lysates?
Our Anti-biotin, HRP-linked Antibody #7075 is intended for use with our Biotinylated Protein Ladder Detection Pack #7727. However, it is possible for this antibody to also detect endogenously bioti...
How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?
We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reagents into single use ...
Will your GFP antibodies detect Turbo GFP?
The GFP Antibody #2555, GFP (4B10) Mouse mAb #2955, GFP (5G4) Mouse mAb #55494, and GFP (D5.1) XP® Rabbit mAb #2956 are not expected to detect Turbo GFP due to low sequence homology.
How are the SignalStar™ Multiplex IHC Kits & Reagents validated?
CST thoroughly validates each antibody available in the PTMScan Discovery uses immunoaffinity LC-MS with motif antibodies to identify and quantify PTMs on cellular proteins. The SignalStar Multiplex IHC kits and reagents have been optimized with respect to fluorophore pairing and order of antibodies. As tissues vary in quality and expression level of biomarkers, increasin... When Goat Anti-Feline IgG, Fc Fragment Antibody (Texas Red Conjugate) #99224 is stored undiluted at 4°C for longer than 6 weeks, there may be a risk of contamination, resulting in a gelatinous/fuzz... The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic elution step, which l... We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include: What is PTMScan® Discovery?
Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?
Are there any issues with storing my Goat Anti-Feline IgG, Fc Fragment Antibody (Texas Red Conjugate) #99224, undiluted, for longer than the recommended conditions?
Can I reuse PTMScan® antibody beads?
Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?
What is the extinction coefficient of my rabbit antibody?
The molar extinction coefficient of rabbit IgG at 280 nm is approximately 210,000 M-1cm-1.
Can I modify the CST-recommended protocol for a given application?
CST antibodies undergo thorough validation processes to ensure reliable performance for every approved application. This includes testing different protocol variations to identify the most effective o...
Have species been tested if they are not listed as cross-reactive with a CST antibody on the datasheet?
Possibly. CST scientists test all our antibodies on multiple species when feasible, depending on the target protein and the experimental systems available. If a tested species does not show cross-reac...
Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?
The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 has been tested succe...
Why do I see a doublet when using your Na,K-ATPase Antibody #3010?
We have found that a doublet may appear if samples are boiled prior to gel loading when performing a western blot with our Na,K-ATPase Antibody #3010. The protein structure of this target is extremel...