Technical Support Articles
Results (694)
Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?
The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 has been tested succe...
Why is a fluorescent conjugate of a rabbit antibody giving no signal on compensation beads in flow cytometry?
If you are using compensation beads in your flow cytometry experiment and observe no signal with one of our fluorescent conjugates of a rabbit antibody, this may be due to the reactivity of the compen...
Can CST develop an antibody for me?
CST does not develop custom antibodies. However, we are happy to accept your target suggestions. Please No materials of animal origin are used in the production of our Human IL-22 Recombinant Protein #74808. Therefore, additional testing for infectious material is not relevant. Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77... The Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 is a mouse mAb from a single clone, whereas the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 is a mix of rabbit mAbs from multiple clon... Unfortunately, due to the nature of lot production, none of the components within the Senescence β-Galactosidase Staining Kit #9860 are available for individual purchase outside of the kit. The selection of a phospho-AMPKα antibody depends on the subunit of interest. Cell Signaling Technology has four antibodies that target phospho-AMPKα:Does the Human IL-22 Recombinant Protein #74808 contain any human or animal material and is it free of HIV/AIDS, HCV/HBC or other infectious material?
Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??
How do the Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 and the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 compare in terms of their sensitivity and specificity?
Can I purchase the components of the Senescence β-Galactosidase Staining Kit #9860 as stand alone products?
Which phospho-AMPKα antibody do you suggest for my western blot experiment?
Can I ship your products to another country?
Our terms of use do not allow for the international shipping of our products after they have been purchased. We have an international distribution network which enables our products to be purchased lo...
Which phospho-Akt (Ser473) antibody do you suggest for Immunofluorescence (Immunocytochemistry) with mouse samples?
We have several antibodies for detecting Akt phosphorylation at Ser473 that are validated for IF-IC with mouse tissues. These are:
- Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060
Why are there background peptides in my PTMScan® experiment?
The background or number of non-PTM peptides in each enrichment is primarily due to non-specific interactions with the beads the antibody is conjugated to. Therefore, this background is also non-deple...
Is Fc blocking needed for F(ab') antibody fragments used in live cell flow cytometry assays?
What controls can I use to show my CUT&RUN experiment is working?
Whether performing CUT&RUN with downstream qPCR or NG-seq analysis, we always recommend using a CUT&RUN-validated positive control antibody like our The typical DNA yields from ChIP experiments range from 0.2-2 ng/µl when targeting transcription factors or cofactors and from 2-20 ng/µl when targeting histone modifications. If the concentration is ... We have several antibodies for detecting total Akt protein that are validated for IF-IC with human samples. These are as follows:
How much DNA is required to make my library for ChIP-seq?
Which total Akt antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
Can you provide data that confirms the specificity of your AMPKα Antibody #2532?
The specificity of our AMPKα Antibody #2532 has been confirmed as follows:
1) siRNA
PMID: 28008135 (https://pubmed.ncbi.nlm.nih.gov/28008135/) Figure 2A
2) shRNA
PMID: 2855...
Is it possible to perform CUT&Tag on samples like heart, brain, or developing mouse tissues?
Yes. We have shown that our CUT&Tag Assay Kit #77552 works with mouse liver, mouse brain, and mouse heart tissues for histone targets with as little as 1 mg of tissue for each CUT&Tag reaction...
Which phospho-Akt (Ser473) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) for mouse samples?
We have several antibodies for detecting Akt phosphorylation at Ser473 that are validated for IHC-P with mouse samples. These are the following:
- Phospho-Akt (Ser473) (D9E) XP® Rab...
What positive and negative controls can I use to demonstrate that my CUT&Tag experiment is successful?
We recommend using a CUT&Tag-validated positive control antibody, like our Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751, in your experiment to confirm that your CUT&Tag assay is worki...
My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?
Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex...
Do you have a protocol for lysing in a 96-well plate (no sonication)?
For lysis in 96-well assay plates, we recommend incubation in the provided lysis buffer for ~30 min at 4°C with gentle shaking. While sonication is difficult in small volumes, we strongly recommend...
How long after the completion of staining can I wait to image my slides when performing a SignalStar™ Multiplex IHC assay?
For Imaging Round 1, the staining should show robust signal when imaged up to 8 hours post completion of staining. For Imaging Round 2, imaging should be performed as close to the completion of staini...
Which p44/42 MAPK (Erk1/2) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several monoclonal antibodies studying p44 and p42 mitogen-activated protein kinases (MAPKs), also referred to as Erk2 and Erk1, that are validated for IHC-P for mouse samples. These are p44/4...
I don’t see my target of interest in your menu of available antibodies for SignalStar™ Multiplex IHC. Can I still use it in my panel in some way?
SignalStar Multiplex IHC kits and reagents haven’t yet been validated for use with antibodies outside of our menu. We’re in the process of developing cust...
What is the shelf life of my product?
Information about the shelf life of our products can be found at the following link: When selecting an antibody for use in CUT&RUN, we recommend starting with a ChIP or ChIP-seq-validated antibody; however, we have found that not all ChIP-validated antibodies work in the CUT&am... While the SignalStain® DAB Substrate Kit #8059 does have a recommended storage temperature of 4°C, we have found tha... Any tissue shown to be positive for each marker via chromogenic IHC can serve as a positive control tissue for a SignalStar™ assay. Each target will therefore require a positive control, which may som... We have several monoclonal antibodies for detecting ribosomal protein S6 that are validated for IF-IC with human samples. These are S6 Ribosomal Protein (5G10) Rabbit mAb #2217 and S6 Ribosomal Pro... We have several antibodies for detecting cleaved PARP that are validated for IF-IC with human samples. These are Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625 and Cleaved-PARP (Asp214) (E2T4K) M...What is the best way to choose an antibody for my CUT&RUN assay if I cannot find a CUT&RUN validated antibody for my target protein?
Will the SignalStain® DAB Substrate Kit #8059 still perform as expected if the material has been frozen?
What is an appropriate positive control to include in this SignalStar™ assay? Are multiple controls necessary?
Which S6 ribosomal protein antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) with human samples?
Which cleaved PARP antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) in human samples?