Technical Support Articles
Results (689)
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p...
Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?
The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:
PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure 2B
PMID: 249...
Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??
Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77...
How should I resuspend and aliquot the PTMScan® LysC Protease #84748?
To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, spin the vial at 1,0...
How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?
We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reagents into single use ...
Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?
Yes, please select “Human” in the first step of the SignalStar Multiplex IHC Panel Builder ...
How are the SignalStar™ Multiplex IHC Kits & Reagents validated?
CST thoroughly validates each antibody available in the CST antibodies undergo thorough validation processes to ensure reliable performance for every approved application. This includes testing different protocol variations to identify the most effective o... The SignalStar Multiplex IHC kits and reagents have been optimized with respect to fluorophore pairing and order of antibodies. As tissues vary in quality and expression level of biomarkers, increasin... We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include: Can I modify the CST-recommended protocol for a given application?
Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?
Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?
How do the Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 and the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 compare in terms of their sensitivity and specificity?
The Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 is a mouse mAb from a single clone, whereas the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 is a mix of rabbit mAbs from multiple clon...
Can I reuse PTMScan® antibody beads?
The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic elution step, which l...
Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&RUN experiments?
The Concanavalin A beads can clump together rather easily, especially when the cells become lysed. To avoid this, make sure your cells are healthy and be sure to treat them very gently during the cell...
Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?
The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 has been tested succe...
Which p44/42 MAPK (Erk1/2) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several monoclonal antibodies studying p44 and p42 mitogen-activated protein kinases (MAPKs), also referred to as Erk2 and Erk1, that are validated for IHC-P for mouse samples. These are p44/4...
Will the Non-phospho (Active) YAP (Ser127) (E6U8Z) Rabbit mAb #29495 cross-react with TAZ?
YAP and TAZ are co-expressed abundantly in every cell line that has been shown to be positive for either protein, and likewise, they are negative/low in the same cell lines (essentially, they appear t...
Does the Human IL-22 Recombinant Protein #74808 contain any human or animal material and is it free of HIV/AIDS, HCV/HBC or other infectious material?
No materials of animal origin are used in the production of our Human IL-22 Recombinant Protein #74808. Therefore, additional testing for infectious material is not relevant.
Do I need to optimize the Tn5 tagmentation conditions for different cell types when performing CUT&Tag?
Our optimized tagmentation conditions for the CUT&Tag assay work well across multiple cell lines, both adherent and suspension. We recommend using 2 μL of CUT&Tag pAG-Tn5 (Loaded) #79561 in...
Which beta-actin antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several antibodies for β-actin that are validated for IHC-P in mouse samples. These are β-Actin (13E5) Rabbit mAb #4970 and β-Actin (8H10D10) Mouse mAb #3700. We would recommend β-Actin (13E5)...
Are any of your SARS-CoV-2 Spike Proteins suitable for cell culture?
Unfortunately, we have not tested our SARS-CoV-2 Spike Proteins in cell culture. Additionally, these products are not sterile, therefore we would not recommend using them in this manner.
Which beta-actin antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have several antibodies for β-actin that are validated for IHC-P in human samples. These are β-Actin (13E5) Rabbit mAb #4970 and β-Actin (8H10D10) Mouse mAb #3700. Both work well and there is no pr...
Which phospho-S6 ribosomal protein (Ser235/236) antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) with mouse samples?
We have several antibodies for detecting the phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 that are validated for IF-IC with mouse samples. These are Phospho-S6 Ribosomal Protein (Ser23...
Why is a fluorescent conjugate of a rabbit antibody giving no signal on compensation beads in flow cytometry?
If you are using compensation beads in your flow cytometry experiment and observe no signal with one of our fluorescent conjugates of a rabbit antibody, this may be due to the reactivity of the compen...
Can CST develop an antibody for me?
CST does not develop custom antibodies. However, we are happy to accept your target suggestions. Please For lysis in 96-well assay plates, we recommend incubation in the provided lysis buffer for ~30 min at 4°C with gentle shaking. While sonication is difficult in small volumes, we strongly recommend... As long as your Concanavalin A Magnetic Beads #82307 have been stored at the appropriate recommended temperature, the appearance of aggregation is not a cause for concern. By nature, the bead particle... CST routinely tests antibodies in relevant applications. Those that pass rigorous application-specific validation standards are recommended. We only recommend applications that have passed rigorous va... The selection of a phospho-AMPKα antibody depends on the subunit of interest. Cell Signaling Technology has four antibodies that target phospho-AMPKα:Do you have a protocol for lysing in a 96-well plate (no sonication)?
Why do my Concanavalin A Magnetic Beads #82307 appear aggregated?
Does this antibody work for my application (western blot, immunoprecipitation, immunofluorescence, immunohistochemistry, ELISA, flow cytometry, chromatin immunoprecipitation, etc.)?
Which phospho-AMPKα antibody do you suggest for my western blot experiment?
Does CUT&RUN show a bias toward euchromatin or heterochromatin?
We do not observe any bias toward euchromatin or heterochromatin in our assays. In CUT&RUN, the target-specific antibody recruits the pAG-MNase and directs digestion to chromatin that is directly ...
Does your CUT&RUN Assay Kit work with isolated nuclei?
Our CUT&RUN Assay Kit is optimized for use with whole cells and works with a large number of cell lines, both adherent and suspension. While it is not typically necessary to pre-isolate cell nucle...