Technical Support Articles
Results (694)
How does the specificity of the F(ab’)2 fragments differ from the corresponding full-length monoclonal antibodies?
Is the “Best By” date the expiration date for the product? Will the product still work after that date?
Our products can be expected to perform at optimal levels up until the printed "Best" by date on the vial when stored under the recommended conditions. Many of our products will work well af...
What is mouse on mouse (MOM) background in IHC and how can it be addressed?
"Mouse on mouse" (MOM) background is caused by the anti-mouse secondary antibody binding to endogenous mouse IgG expressed in the tissue resulting in non-specific staining, which can complic...
Why do your Rad18 antibodies detect two bands by western blot?
We believe the upper band observed when using the Rad18 (D2B8) XP® Rabbit mAb #9040, Phospho-Rad18 (Ser403) (D2T6W) Rabbit mAb #14978, or Phospho-Rad18 (Ser403) Antibody #8393 is likely mono-ubiquitin...
Do your IRAK4 Antibody #4363 and IRAK-M Antibody #4369 cross-react with other IRAK family members?
The antigenic peptides used to produce our IRAK4 Antibody #4363 and our IRAK-M Antibody #4369 have been carefully selected to avoid cross-reactivity with the other IRAK family members. Our IRAK-M Anti...
What is the concentration of the pAG-MNase Enzyme #57813?
The concentration of the pAG-MNase Enzyme #57813 in our CUT&RUN kits is 25ug/ml.
Can the Phospho-β-Catenin (Ser33/37) Antibody #2009 bind if the sites are singly phosphorylated or is the phosphorylation at both sites necessary?
We have tested the reactivity of the Phospho-β-Catenin (Ser33/37) Antibody #2009 to Ser33/37 by peptide dot blot. This antibody showed strong signal when phosphorylated at Ser37 alone and when phospho...
Are LICOR buffers compatible with CST products in fluorescent western blot?
While we strongly urge customers to continue using our recommended blotting buffers, it has been found that the TBS based LICOR buffers also appear to be compatible with our products in the fluorescen...
What is the benefit of carrier vs. carrier free cytokines?
Our cytokines and growth factors are lyophilized and supplied either with 20ug BSA per 1ug of cytokine (with carrier) or without added protein (carrier free). BSA minimizes adsorption to surfaces and ...
Why are the beads from PTMScan® HS Kits aggregating against the sides of the tube?
Through in-house testing, we have found that the magnetic beads from our PTMScan HS kits aggregate and stick to the walls of some specific brands of tubes. Moreover, they cannot be washed off or spun ...
Can the Lambda Protein Phosphatase Kit #89726 be stored at -20°C?
The Lambda Protein Phosphatase #56206 included in the Lambda Protein Phosphatase Kit #89726 is temperature sensitive. Therefore, we recommend storing the kit at -80°C. However, the kit is stable at -2...
What secondary antibodies do you recommend for fluorescent western blotting?
We currently have four different secondary antibodies approved for use in fluorescent western blotting, depending on species and wavelength of detection:
- Anti-mouse IgG (H+L) (DyLight™ 680 Con...
Has your Sox2 (D6D9) XP® Rabbit mAb #3579 been tested for cross-reactivity with other SOX family members?
We have not tested our Sox2 (D6D9) XP® Rabbit mAb #3579 for cross-reactivity with other SOX family transcription factors. However, sequence alignments have been done between the antigen used to produc...
Why do I see a doublet with my beta-Actin antibody?
It is not uncommon to see a doublet with our beta-Actin antibodies depending on the samples being tested. It is likely caused either by phosphorylation of beta-Actin on its many phosphorylation sites,...
Can I ship your products to another country?
Our terms of use do not allow for the international shipping of our products after they have been purchased. We have an international distribution network which enables our products to be purchased lo...
Are any of your SARS-CoV-2 Spike Proteins suitable for cell culture?
Unfortunately, we have not tested our SARS-CoV-2 Spike Proteins in cell culture. Additionally, these products are not sterile, therefore we would not recommend using them in this manner.
What procedure do you recommend for antigen retrieval with EDTA?
We recommend using a microwave to bring slides to a boil in 1 mM EDTA, pH 8.0 followed by 15 minutes at sub-boiling temperature. Typically the power level must be adjusted during the 15 minute period ...
Which isotype control should I use for IHC?
For mouse IgGs, you will need to determine the isotype of the primary antibody and use the appropriate isotype control:
IgG1: Mouse (G3A1) mAb IgG1 Isotype Control #5415
IgG2a: Mouse (E5Y6Q) mAb...
Why do your Glucocorticoid Receptor (D6H2L) XP® #12041 and Glucocorticoid Receptor (D8H2) XP® #3660 Rabbit mAbs detect two bands at 91 and 94 kDa?
From our testing, we have seen that our Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb #12041 detects both the alpha and beta isoforms of the Glucocorticoid Receptor. It is possible that #12041 may de...
Do I need to optimize the Tn5 tagmentation conditions for different cell types when performing CUT&Tag?
Our optimized tagmentation conditions for the CUT&Tag assay work well across multiple cell lines, both adherent and suspension. We recommend using 2 μL of CUT&Tag pAG-Tn5 (Loaded) #79561 in...
Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&Tag experiments?
Concanavalin A beads can clump together rather easily, especially when cells become lysed. Make sure your cells are healthy and be sure to treat them very gently during the cell wash. We also recommen...
What is the binding capacity of the antibody-bead conjugates for the agarose PTMScan® kits?
The background binding of non-PTM peptides is typically 50% or more of the total peptides bound to the antibody beads in our agarose PTMScan® kits. This has been improved to less than 50% background o...
Can I use PFA as a fixative rather than the fixative provided with the Senescence β-Galactosidase Staining Kit #9860?
We have not tested samples that have been fixed with paraformaldehyde so we cannot guarantee the kit will work using this fixative. For reference, our Fixative Solution #11674 which is supplied with t...
Do your Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAbs detect both the alpha and beta isoforms?
Our Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAbs #11818 and #42801 detect both phospho-ACC-alpha and phospho-ACC-beta. These antibodies recognize ACC-alpha only when phosphorylated at Se...
Which beta-actin antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several antibodies for β-actin that are validated for IHC-P in mouse samples. These are β-Actin (13E5) Rabbit mAb #4970 and β-Actin (8H10D10) Mouse mAb #3700. We would recommend β-Actin (13E5)...
Can the dot blot protocol be modified if I don't have a dot blotting apparatus for use with your 5-Methylcytosine (5-mC) (D3S2Z) Rabbit mAb #28692?
If a dot blotting apparatus is not available for use, we recommend denaturing your DNA in a lower volume/higher concentration (see modified steps below):
Section B. Dot Blot Modified Steps:
1. D...
Do you have a recommended protocol for stripping/re-probing blots?
The western blot stripping/re-probing protocol for our Western Blot Stripping Buffer (5X) #91925 is below. However, it is important to note that we typically advise against stripping/re-probing becaus...
Which phospho-S6 ribosomal protein (Ser235/236) antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) with mouse samples?
We have several antibodies for detecting the phosphorylation of ribosomal protein S6 (rpS6) at Ser235/236 that are validated for IF-IC with mouse samples. These are Phospho-S6 Ribosomal Protein (Ser23...
Do you observe any differences in CUT&Tag assay performance among different cell lines?
We have performed CUT&Tag on dozens of cultured adherent and suspension cell lines, both human and mouse. So far, we have not noticed any significant difference in performance between these cell l...
Which beta-actin antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have several antibodies for β-actin that are validated for IHC-P in human samples. These are β-Actin (13E5) Rabbit mAb #4970 and β-Actin (8H10D10) Mouse mAb #3700. Both work well and there is no pr...