Technical Support Articles
Results (713)
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic deterg...
Do I need to optimize the SignalStar™ Multiplex IHC Kits & Reagents for the type of tissue I’m using?
The SignalStar Multiplex IHC kits and reagents have been optimized with respect to fluorophore pairing and order of antibodies. As tissues vary in quality and expression level of b...
Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??
Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&a...
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with mouse samples. These are:
- Cleaved Caspase-3 (Asp175) Antibody #9...
What is the solution formulation of the PTMScan Control peptides?
The solution formulation of the PTMScan Control peptides is 25% acetonitrile, 74.9% water, 0.1% trifluoroacetic acid (V:V).<...
Does this antibody work for my application (western blot, immunoprecipitation, immunofluorescence, immunohistochemistry, ELISA, flow cytometry, chromatin immunoprecipitation, etc.)?
CST routinely tests antibodies in relevant applications. Those that pass rigorous application-specific validation standards are recommended. We only recommend applications that hav...
Why is the concentration of my antibody so low?
At CST, our antibodies are formulated on the basis of their reactivity and performance for each approved application at a recommended dilution. For antibodies with high affinit...
What controls should I include when performing a Simple Western™ experiment?
In addition to a known positive control sample, we recommend the following negative controls for your Simple Western™ experiment.
1. A no lysate control. This includes the p...
Should digitonin be included in my wash buffers for my entire CUT&Tag experiment?
Yes, digitonin needs to be present in all wash buffers during the entire CUT&Tag experiment. This is because cell membrane permeabilization caused by digitonin is reversible. R...
What positive and negative controls can I use to demonstrate that my CUT&Tag experiment is successful?
We recommend using a CUT&Tag-validated positive control antibody, like our Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751, in your experiment to confirm that your CUT&am...
Which cleaved PARP antibody do you suggest for immunofluorescent immunocytochemistry (IF-IC) in human samples?
We have several antibodies for detecting cleaved PARP that are validated for IF-IC with human samples. These are Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625 and Cleaved-PAR...
Will the Non-phospho (Active) YAP (Ser127) (E6U8Z) Rabbit mAb #29495 cross-react with TAZ?
YAP and TAZ are co-expressed abundantly in every cell line that has been shown to be positive for either protein, and likewise, they are negative/low in the same cell lines (essent...
What types of Ras are recognized by your G12D and G12V mutant specific antibodies?
Our Ras (G12D Mutant Specific) (D8H7) Rabbit mAb #14429 and Ras (G12V Mutant Specific) (D2H12) Rabbit mAb #14412 will detect all three types of Ras (H-Ras, K-Ras, and N-Ras) if the...
Why isn't my bottle of Cell Lysis Buffer (10X) #9803 frozen?
Occasionally, Cell Lysis Buffer (10X) #9803 stored at -20C may not appear frozen, which is not a reason for concern. This phenomenon is known as nucleation, or the initial formatio...
Why do I see high background by western blot when probing for phospho-AMPK (Thr172)?
If CST recommended buffers are being used for western blotting and high background or additional bands persist when probing for phospho-AMPK (Thr172), the issue could be related to...
Does your Insulin (C27C9) Rabbit mAb #3014 detect proinsulin?
Our Insulin (C27C9) Rabbit mAb #3014 was produced with an antigenic peptide corresponding to residues within the insulin B chain of the human insulin protein (UniProt ID #P01308; h...
What is the difference between your SimpleChIP® ChIP-seq Single Index Primers #29580 and SimpleChIP® ChIP-seq Dual Index Primers #47538?
Both SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Single Index Primers) #29580 and SimpleChIP® ChIP-seq Multiplex Oli...
What PD-L2 antibodies do you currently offer?
We currently offer three products targeting PD-L2:
Our PD-L2 (D7U8C) XP® Rabbit mAb #82723 recognizes endogenous levels of total human PD-L2 protein and was produced by immu...
What are the differences between the BrdU Cell Proliferation Chemiluminescent Assay Kit #5492 and the BrdU Cell Proliferation Assay Kit #6813? Which should I choose?
In general, the BrdU Cell Proliferation Chemiluminescent Assay Kit #5492 offers a broader dynamic range and higher sensitivity compared to the BrdU Cell Proliferation Assay Kit #68...
Are the Protein A Magnetic Beads #73778 and the Protein G Magnetic Beads #70024 hydrophilic or hydrophobic?
The base particle of the Protein A Magnetic Beads #73778 and the Protein G Magnetic Beads #70024 is somewhat hydrophilic. It is difficult to predict the hydrophilicity or hydrophob...
How should I resuspend and aliquot the PTMScan® LysC Protease #84748?
To resuspend the PTMScan® LysC Protease #84748, we recommend allowing the vial to equilibrate at room temperature for a few minutes after removing from the -20C freezer. Next, ...
Can you provide data that confirms the specificity of your Raptor (24C12) Rabbit mAb #2280?
The specificity of our Raptor (24C12) Rabbit mAb #2280 has been confirmed by siRNA in the following papers:
PMID: 28648777 (https://pubmed.ncbi.nlm.nih.gov/28648777/) Figure...
How stable is the Senescence β-Galactosidase Staining Kit #9860 after multiple freeze/thaws?
We are confident that the Senescence β-Galactosidase Staining Kit #9860 will continue to perform as expected up to at least 3 freeze/thaw cycles. We recommend aliquoting your reage...
How are the SignalStar™ Multiplex IHC Kits & Reagents validated?
CST thoroughly validates each antibody available in the CST antibodies undergo thorough validation processes to ensure reliable performance for every approved application. This includes testing different protocol variations to identify ... We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include:
Can I modify the CST-recommended protocol for a given application?
Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?
Is the VersicanG1-Fc (Rabbit IgG1) #15933 product homologous with all species?
The VersicanG1-Fc (Rabbit IgG1) #15933 contained in the Hyaluronan Complete Tissue Staining Kit (HRP) #38822 and Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721 h...
Can I order a custom formulation of an antibody?
For custom (carrier-free) orders, many antibodies are available pre-formulated in PBS without BSA or glycerol. This carrier-free formulation is amenable to conjugation and can be c...
In terms of cytokine activity, what is the difference between International Units (IU) and specific activity?
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International Units (IU) and specific activity are two differe... Can I reuse PTMScan® antibody beads?The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic e...
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