Aphidicolin #32774
Product Information
Product Usage Information
Aphidicolin is supplied as a lyophilized powder. For a 5 mM stock, reconstitute 1 mg of powder in 590 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.
Storage
Product Description
| Molecular Weight | 338.5 g/mol |
| Purity | >98% |
| Molecular Formula | C20H34O4 |
| CAS | 38966-21-1 |
| Solubility | Soluble in DMSO at 10 mg/ml or ethanol at 1 mg/ml. |
Background
Aphidicolin is a tetracyclic diterpenoid first produced by the fungus Cephalosporium aphidicola. This antibiotic is a potent inhibitor of cellular deoxyribonucleic acid (DNA) synthesis by targeting α, ε, and δ DNA polymerases in eukaryotic cells lines with little to no effect on β or γ DNA polymerases (1,2). Studies have shown that Aphidicolin specifically binds to α DNA polymerase, resulting in the formation of a pol α-DNA-aphidicolin ternary complex that blocks DNA replication. The uses for this compound include synchronized cells at the G1/S boundary and increased gene amplification (3-6).
- Longiaru, M. et al. (1979) Nucleic Acids Res 6, 3369-86.
- Syväoja, J. et al. (1990) Proc Natl Acad Sci U S A 87, 6664-8.
- Dinter-Gottlieb, G. and Kaufmann, G. (1983) J Biol Chem 258, 3809-12.
- Sheaff, R. et al. (1991) Biochemistry 30, 8590-7.
- Kota, K.P. et al. (2012) Viruses 4, 1865-77.
- Yin, D.X. and Schimke, R.T. (1996) Proc Natl Acad Sci U S A 93, 3394-8.
Limited Uses
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