Cat. # | Size | Qty. | Price |
---|---|---|---|
69824C | 1 Kit (96 assays) |
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When ordering five or more kits, please contact us for processing time and pricing.
Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.
REACTIVITY | H |
Product Includes | Volume (with Count) | Solution Color | |||
---|---|---|---|---|---|
FastScan™ ELISA Microwell Strip Plate, 96 Well 53257 | 1 x 96 tests | ||||
Syk Rabbit Capture mAb | 1 x 1 ea | Green (Lyophilized) | |||
Syk Mouse HRP-linked mAb | 1 x 1 ea | Red (Lyophilized) | |||
FastScan™ ELISA Capture Antibody Diluent | 1 x 3 ml | Green | |||
FastScan™ ELISA HRP Antibody Diluent | 1 x 3 ml | ||||
TMB Substrate 7004 | 1 x 11 ml | ||||
STOP Solution 7002 | 1 x 11 ml | ||||
Sealing Tape | 1 x 1 ea | ||||
ELISA Wash Buffer (20X) 9801 | 1 x 25 ml | ||||
FastScan™ ELISA Cell Extraction Buffer (5X) 69905 | 1 x 10 ml | ||||
FastScan™ ELISA Cell Extraction Enhancer Solution (50X) 25243 | 1 x 1 ml | ||||
FastScan™ ELISA Kit #69824 Positive Control Type 2 | 1 x 1 ea |
Product Information
NOTE: Prepare solutions with deionized/purified water or equivalent.
Prepare only as much reagent as needed on the day of the experiment.
*IMPORTANT: The provided FastScan™ ELISA Cell Extraction Enhancer Solution (50X) may precipitate when stored at 4°C. To dissolve, warm briefly at 37°C and mix gently. The FastScan™ ELISA Cell Extraction Enhancer Solution (50X) can be stored at room temperature to avoid precipitation.
NOTE: The 1X Cell Extraction Buffer contains phosphatase inhibitors. Protease inhibitors should be added to the 1X Cell Extraction Buffer immediately prior to lysing cells. Additional phosphatase inhibitors can also be added (e.g. Protease/Phosphatase Inhibitor Cocktail (100X) #5872, not supplied).
NOTE: A select number of FastScan™ ELISA kits do not contain a positive control, please refer to Product Includes table on the datasheet for a list of included reagents. Should you need support on how to generate a positive control for those kits, contact CST technical support at [email protected].
For adherent cells
For suspension cells
NOTE: Equilibrate all materials and prepared reagents to room temperature prior to running the assay.
*NOTE: Certain FastScan™ ELISA Kits may require additional washes at this step. Any requirements for additional washes will be specifically noted in the product “Description” of the kit’s datasheet.
NOTE: Initial color of positive reaction is blue, which changes to yellow upon addition of STOP Solution.
posted May 2018
revised November 2019
Protocol Id: 1924
Syk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells and investigators have indicated that Syk is a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation at Tyr323 provides a direct binding site for the TKB domain of Cbl (6,7). Tyr352 of Syk is involved in the association of PLCγ1 (8). Tyr525 and Tyr526 are located in the activation loop of the Syk kinase domain; phosphorylation at Tyr525/526 of human Syk (equivalent to Tyr519/520 of mouse Syk) is essential for Syk function (9).
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