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AFAP1L2/XB130 (D1B5) Rabbit Monoclonal Antibody #12684

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  • WB
  • IP

    Product Specifications

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 130
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    AFAP1L2/XB130 (D1B5) Rabbit Monoclonal Antibody recognizes endogenous levels of total AFAP1L2/XB130 protein. In some cell extracts this antibody recognizes an 80 kDa background protein of unknown origin.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly638 of human AFAP1L2/XB130 protein.

    Background

    The actin-filament associated protein (AFAP) family consists of AFAP1, AFAP1L1, and AFAP1L2/XB130, a group of structurally similar proteins that play distinct roles in the regulation of cytoskeletal dynamics and signal transduction. Actin filament-associated protein 1-like 2 (AFAP1L2, XB130) is an adaptor protein that regulates signaling downstream of multiple kinases, including Src, Akt, and the thyroid specific kinase RET/PTC (1-3). Through these pathways, AFAP1L2/XB130 mediates transcriptional regulation, cell proliferation, motility, and microRNA expression (4,5). Research has shown that AFAP1L2/XB130 is involved in the proliferation and survival of thyroid tumor cells (6), and may have value in gastric cancer prognosis (7).

    Alternate Names

    actin filament associated protein 1 like 2; actin filament associated protein 1-like 2; Actin filament-associated protein 1-like 2; AF1L2; AFAP1-like protein 2; AFAP1L2; CTB-1144G6.4; CTB-1144G6.6; FLJ14564; KIAA1914; XB130

    For Research Use Only. Not for Use in Diagnostic Procedures.
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