AGPAT2 (D8W9B) Rabbit mAb #14937
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 27 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Protocol
Specificity / Sensitivity
AGPAT2 (D8W9B) Rabbit mAb recognizes endogenous levels of total AGPAT2 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly235 of human AGPAT2 protein.
Background
The 1-acylglycerol-3-phosphate-O-acyltransferase 2 enzyme (AGPAT2) catalyzes the acylation of lysophosphatidic acid (LPA) into phosphatidic acid (PA), which is a precursor for the synthesis of triacylglycerol and phospholipid (1,2). AGPAT2 is highly expressed in adipose tissues, liver, and skeletal muscle (3). The induced knockdown of AGPAT2 expression results in decreased expression of adipogenic proteins and delayed expression of adipogenic marker proteins, suggesting that AGPAT2 plays an important role in adipocyte growth and differentiation (4). Mutations in the corresponding AGPAT2 gene cause autosomal recessive congenital generalized lipodystrophy type 1 (CGL1), also described as Berardinelli-Seip syndrome. Patients with CGL1 are born without detectable white adipose tissue and tend to develop severe insulin resistance, hypertriglyceridemia, and type 2 diabetes during childhood (5,6).
Limited Uses
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