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  1. Products /
  2. Primary Antibodies /
  3. AHCYL1/IRBIT (D3A5G) Rabbit mAb
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

AHCYL1/IRBIT (D3A5G) Rabbit mAb #94248

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  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 61
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    AHCYL1/IRBIT (D3A5G) Rabbit mAb recognizes endogenous levels of total AHCYL1/IRBIT protein. This antibody does not cross-react with AHCYL2/IRBIT2 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu50 of human AHCYL1/IRBIT protein.

    Background

    S-adenosylhomocysteine hydrolase-like protein 1 (AHCYL1) is a member of S-adenosylhomocysteine hydrolase family, which participates in the metabolism of S-adenosyl-L-homocysteine (1). Two Drosophila homologs of S-adenosylhomocysteine hydrolase-like proteins, dAhcyL1 and dAhcyL2, were identified as novel components of methionine metabolism (2). dAhcyL1 and dAhcyL2 function as dominant-negative regulators of S-adenosylhomocysteine hydrolase (2). Global down-regulation of both dAhcyL1 and dAhcyL2 extended life span (2). In addition, brain-specific down regulation of dAhcyL1 extended life span (2). AHCYL1 is also known as inositol 1,4,5-trisphosphate receptor (IP3R) binding protein released with IP3 (IRBIT) (1, 3). This protein binds to the endoplasmic reticulum calcium release channel IP3R and represses its acitivity (1, 3). As a multifunctional regulator, AHCYL1/IRBIT can also form a complex with and suppress the activity of ribonucleotide reductase, thereby influencing the balance of deoxynucleotide triphosphates essential for DNA replication and genomic integrity (4).

    Alternate Names

    1-C; 1C; adenosylhomocysteinase-like 1; AdoHcyase 2; AHCYL1; DC-expressed AHCY-like molecule; DCAL; dendritic cell expressed AHCY-like protein; inositol 145-trisphosphate receptor-binding protein; IRBIT; PRO0233; Putative adenosylhomocysteinase 2; S-adenosyl homocysteine hydrolase homolog; S-adenosyl-L-homocysteine hydrolase 2; S-adenosylhomocysteine hydrolase-like 1; S-adenosylhomocysteine hydrolase-like protein 1; SAHH2; XPVKONA

    Database Links

    UniProt ID: O43865


    Entrez-Gene Id: 10768


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