APS Antibody #4832
- WB
- IHC
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 90 to 95 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
APS Antibody detects endogenous levels of total APS. This antibody does not cross-react with other adaptor/docking proteins.
Species Reactivity:
Human, Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human APS. Antibodies are purified by protein A and peptide affinity chromatography.
Background
APS is an SH2 and PH domain-containing adaptor protein closely related to Lnk and SH2-B (1). APS was identified as a substrate for many receptor tyrosine kinases including TrkA, insulin receptor, c-Kit and PDGF receptor (2). Tyrosine phosphorylation of APS provides docking sites for downstrean signaling components, mediating diverse signaling pathways. APS plays quite different roles in RTK signaling. Overexpression of APS has been shown to inhibit PDGF-induced mitogenicity, which may result from APS/c-Cbl-mediated PDGF receptor degradation (3). However, APS promotes enhanced mitogenicity in response to insulin stimulation (4). The striking difference in APS-mediated signaling between the different RTKs could lie in the mode of interaction with the respective receptor.
Limited Uses
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