Cat. # | Size | Qty. | Price |
---|---|---|---|
9946T | 1 Kit (8 x 20 microliters) |
|
Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
---|---|---|---|---|---|
Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody 9464 | 20 µl |
|
H M R Mk | 78 to 82, 95 | Rabbit |
Phospho-FoxO1 (Ser256) Antibody 9461 | 20 µl |
|
H M R Mk | 82 | Rabbit |
Phospho-FoxO1 (Thr24)/FoxO3a (Thr32)/FoxO4 (Thr28) (4G6) Rabbit mAb 2599 | 20 µl |
|
H M Mk | 65, 78 to 82, 95 | Rabbit |
FoxO1 (C29H4) Rabbit mAb 2880 | 20 µl |
|
H M R Mk | 78 to 82 | Rabbit IgG |
Phospho-FoxO3a (Ser253) (D18H8) Rabbit mAb 13129 | 20 µl |
|
H M R Mk | 97 | Rabbit IgG |
Phospho-FoxO3a (Ser318/321) Antibody 9465 | 20 µl |
|
H M R Mk | 97 | Rabbit |
FoxO4 Antibody 9472 | 20 µl |
|
H M R Mk | 65 | Rabbit |
FoxO3a (D19A7) Rabbit mAb 12829 | 20 µl |
|
H M R | 82 to 97 | Rabbit IgG |
Anti-rabbit IgG, HRP-linked Antibody 7074 | 100 µl |
|
Goat |
Product Information
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr28 of human Fox04, with a synthetic phosphopeptide corresponding to residues around Ser256 of human Fox01, or with a synthetic peptide corresponding to residues of human Fox04. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with recombinant protein specific to the carboxy terminus of human FoxO3 protein, with a GST-fusion protein corresponding to carboxy-terminal residues of human FoxO1, with a synthetic phosphopeptide corresponding to the sequence of human Fox03a, or with a synthetic phosphopeptide corresponding to residues surrounding Thr28 of human Fox04.
The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).
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