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71633
MRP4/ABCC4 (D1Z3W) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody
R
Recombinant

MRP4/ABCC4 (D1Z3W) Rabbit mAb (BSA and Azide Free) #71633

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  1. WB
  2. IHC
Western Blotting Image 1: MRP4/ABCC4 (D1Z3W) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from membrane fractions of SW620, LNCaP, and HT-29 cells using MRP4/ABCC4 (D1Z3W) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: MRP4/ABCC4 (D1Z3W) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using MRP4/ABCC4 (D1Z3W) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: MRP4/ABCC4 (D1Z3W) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded cell pellets, HT-29 (left) and U-2 OS (right), using MRP4/ABCC4 (D1Z3W) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: MRP4/ABCC4 (D1Z3W) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using MRP4/ABCC4 (D1Z3W) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). Data were generated using the standard formulation of this product.
To Purchase # 71633
Cat. # Size Qty. Price
71633SF
100 µg
$ 758

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 140-200
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #12857. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #12857

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

MRP4/ABCC4 (D1Z3W) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total ABCC4 protein. This antibody cross-reacts with a nonspecific band at 47 kDa in some cell lines.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly192 of human ABCC4 protein.

Background

ABCC4 is a member of the ATP-binding Cassette (ABC) transporter family. ABC proteins transport various molecules across cellular membranes by utilizing the energy generated from ATP hydrolysis. There are seven subfamilies of ABC proteins: ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White (1). ABCC4 belongs to the MRP subfamily, which is involved in multi-drug resistance, hence it is also named MRP4. ABCC4 is widely expressed in tissues including prostate, kidney proximal tubules, astrocytes and capillary endothelial cells of the brain, platelets, and many cancer cell lines (2-4). ABCC4 mediates efflux transport of a wide variety of endogenous and xenobiotic organic anionic compounds (5). The diversity of substrates determines the biological functions of ABCC4. It regulates cAMP levels in human leukemia cells, thereby controlling the proliferation and differentiation of leukemia cells (6). ABCC4 also enables COX deficient pancreatic cancer cells to obtain exogenous prostaglandins (7). Research studies have shown that ABCC4 expression is elevated in drug resistant cancer cells, which makes it a potential target for cancer therapy (8,9). ABCC4 localizes to both plasma membrane and intracellular membranous structures (10). Investigators have also implicated ABCC4 in the pathogenesis of Kawasaki disease, a childhood genetic disorder characterized by vasculitis (11).
  1. Nakanishi, T. Cancer Genomics Proteomics 4, 241-54.
  2. Kool, M. et al. (1997) Cancer Res 57, 3537-47.
  3. Lee, K. et al. (1998) Cancer Res 58, 2741-7.
  4. Nies, A.T. et al. (2004) Neuroscience 129, 349-60.
  5. Giacomini, K.M. et al. (2010) Nat Rev Drug Discov 9, 215-36.
  6. Copsel, S. et al. (2011) J Biol Chem 286, 6979-88.
  7. Omura, N. et al. (2010) Mol Cancer Res 8, 821-32.
  8. Bronger, H. et al. (2005) Cancer Res 65, 11419-28.
  9. Hagmann, W. et al. (2009) Pancreatology 9, 136-44.
  10. Rius, M. et al. (2008) J Pharmacol Exp Ther 324, 86-94.
  11. Khor, C.C. et al. (2011) J Med Genet 48, 467-72.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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