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  3. PTMScan® Lys-C Protease

PTMScan® Lys-C Protease #39003

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Product Name

84748 PTMScan® LysC Protease

Inquiry Info. # 39003

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    Product Information

    Product Usage Information

    For PTMScan® protocols we recommend making a 5 mg/ml stock solution by resuspending 1.2 mg in 0.24 ml of 20 mM HEPES pH 8.0, aliquot for single use and store at -80°C. See the PTMScan® protocol for further details.

    Storage

    Store lyophilized Lys-C at -20°C. Once reconstituted, the enzyme can be aliquoted and stored at -80°C to help avoid damaging freeze thaw cycles. Lyophilized Lys-C has a shelf life of 1 year at -20°C and solutions are stable for 6 months at -80°C.

    Product Description

    Lysl-endopeptidase (Lys-C) hydrolyzes amide and peptide ester bonds on the carboxyl side of lysine residues and hydrolyzes S-aminoethylcysteine residues.

    Specificity / Sensitivity

    The activity of Lys-C is greater than or equal to 3 AU/mg.

    Source / Purification

    Lys-C was produced recombinantly in E. coli, purified and is provided lyophilized in 25 mM Tris-HCl and mannitol. Purity is >90% by SDS-PAGE. One amidase unit (AU) is the amount of enzyme that produces 1 micromole of Ac-Lys-pNA hydrochloride salt per minute at pH 9.5 at 30°C.

    Background

    Lysl-endopeptidase (Lys-C) cleaves proteins on the carboxyl side of lysine residues. This makes it a useful tool in protein sequencing and proteomic applications. The enzyme was originally purified from the Gram-negative soil bacteria Achromobacter lyticus (1). PTMScan® Lys-C is produced recombinantly in E. coli. It has been found to work optimally at the temperatures of 30-37°C and pH ranges from 9.0-9.5 but will undergo degradation at temperatures of 50°C and higher. Activity is maintained in 4M urea or 0.1% SDS solutions for up to 6 hours at 30°C. Inhibitors of the enzyme include PMSF, DFP, and TLCK.

    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not for Use in Diagnostic Procedures.
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    PTMScan is a registered trademark of Cell Signaling Technology, Inc.
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