Custom Antibody Conjugation & Labeling Services

CST has 2,000+ ready-to-ship antibody conjugates for flow cytometry, immunofluorescence, immunohistochemistry, high-content screening, and other immunoassays.

Can’t find the antibody conjugated to the label you need? Our direct conjugation experts can also build specific antibody-label combinations so that you can sidestep the time sink and pitfalls of do-it-yourself kits.

Broad Offering of Labels and Services for Antibody Conjugation

Our general conjugation offering includes fluorescent dyes, biotin, europium, horseradish peroxidase (HRP), beads, and oligonucleotides. See our tiered service options below for more details and additional labels.

Why Choose CST^® Antibody Conjugation and Labeling Services?

• Validation: Size exclusion chromatography ensures conjugated antibodies retain physical integrity and are free of contaminants, such as antibody aggregates or fragments, that may interfere with your experiments. Select application-specific testing using biologically relevant cell systems is also available.
• Optimization: Multiple conjugation chemistries (including site-specific chemistry), stringent purification to remove free dye/label and antibody, and optimal degree of labeling identification maximize signal-to-noise ratio and product performance.
• Flexibility: Multiple tiered service options, from basic antibody conjugation to full validation, to match your needs and budget.
• Guaranteed Quantity: Antibody loss occurs during both the conjugation and cleanup process. We account for varying labeling efficiencies, absorb the cost of excess antibody required to manufacture your conjugate, and ensure you receive exactly the amount of antibody you requested.
• Technical Support: Comprehensive support and consultation from the same scientists who produce and validate CST antibodies and conjugates.

Basic conjugations typically ship in 10-12 days, complex conjugations may take 3-4 weeks.

Request a Custom Conjugation

Superior Performance Over Do-it-Yourself (DIY) Antibody Conjugation

CST Custom Conjugation vs. Other Vendors: Analysis of FGF Receptor 1 (D8E4) XP^® Rabbit mAb #9740 conjugated using CST methods compared to DIY kits from two other vendors via Ultra-High-Performance Liquid Chromatography (UHPLC). The optimization of bio-conjugation chemistries and stringent purification to remove free dye and antibody performed by CST results in a higher quality product, compared to other vendors’ products that contain higher levels of free dye and debris which can interfere with assay results.

Optimization of Degree of Labeling (DOL) Ensures Specific Signal Intensity

DOL Service Option for Fluorophores: Conjugation to certain fluorescent dyes (e.g. Alexa Fluor^® and Pacific Blue™) is optimized by DOL testing to identify the optimal antibody:dye molecular ratio, resulting in conjugates with maximum specific signal intensity. In the data shown, the DOL of 4.28 provides the most optimal signal-to-noise compared to the higher DOLs.

Standard Antibody Conjugation Services Offered

If you don’t see the label you’re interested in, just ask us! In many cases, we can accommodate your request.

¹Standard validation application method is Flow Cytometry. Alternatives may increase timeline and pricing.

²Standard validation application method is Western Blot. Alternatives may increase timeline and pricing.

³Standard validation application method is Immunoprecipitation. Alternatives may increase timeline and pricing.

⁴ Europium conjugates can be paired with CST off-the-shelf or custom-made Alexa Fluor 647 and APC conjugates, providing both the donor and acceptor for TR-FRET assays.

⁵Oligonucleotide conjugation offerings may be limited for certain Intellectual Property (IP)-protected technologies.

_{Regarding Alexa Fluor conjugation, this product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or [email protected]. Alexa Fluor is a registered trademark and Pacific Blue is a trademark of Life Technologies Corporation. Cy and CyDye are registered trademarks of GE Healthcare. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.}

_{Visit cellsignal.com/trademarks for more information.}