IMPORTANT: Please refer to the APPLICATIONS section on the front page of product datasheet or product webpage to determine if this product is validated and approved for use in Flow Cytometry (F). This protocol is provided as a general approach for combined staining in whole blood, but CST does not validate all Flow approved antibodies for use with this protocol. Please see the product-specific Flow protocol on the product webpage for appropriate fixation and permeabilization conditions, and recommended antibody dilution.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Account for isotype-matched controls for monoclonal antibodies or species matched IgG for polyclonal antibodies.
Reference: Chow S, Hedley D, Grom P, Magari R, Jacobberger JW, Shankey TV (2005) Whole blood fixation and permeabilization protocol with red blood cell lysis for flow cytometry of intracellular phosphorylated epitopes in leukocyte subpopulations. Cytometry A 67(1), 4–17.
posted November 2008
revised July 2017