Product Pathways - Cytoskeletal Signaling
Caldesmon-1 (D5C8D) XP® Rabbit mAb #12503
|12503S||100 µl (10 western blots)||---||In Stock||---|
|12503P||40 µl (4 western blots)||---||In Stock||---|
|12503||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||70-80 nonmuscle, 120-150 smooth muscle||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Caldesmon-1 (D5C8D) XP® Rabbit mAb recognizes endogenous levels of total caldesmon-1 protein. Based on sequence homology, the antibody is expected to cross-react with both the smooth muscle and nonmuscle isoforms.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human caldesmon-1 protein.
Western blot analysis of extracts from various cell lines using Caldesmon-1 (D5C8D) XP® Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Immunoprecipitation of caldesmon-1 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Caldesmon-1 (D5C8D) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Caldesmon-1 (D5C8D) XP® Rabbit mAb.
Confocal immunofluorescent analysis of HeLa (left) and MCF7 (right) cells using Caldesmon-1 (D5C8D) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Caldesmon-1 is an actin filament stabilizing protein involved in the regulation of cell contraction. Binding of caldesmon-1 to actin is weakened by phosphorylation and by calmodulin in the presence of calcium. Caldesmon-1 is encoded by a single gene, which is spliced to generate a widely distributed low molecular weight form and a smooth muscle specific high molecular weight form (1,2). Caldesmon-1 is phosphorylated by the cyclin dependent kinase cdc2 and Erk1/2 MAP kinase, both of which prevent the activity of caldesmon-1 (3-5). Phosphorylation of caldesmon-1 by cdc2 is required for passage of cells through mitosis (6). Phosphorylation by Erk1/2 is important in regulating smooth muscle contraction (7). Caldesmon-1 activity may play a role in the formation of podosomes, adhesion complexes associated with the secretion of matrix metalloproteases, invasion, and metastasis (reviewed in 5).
- Hayashi, K. et al. (1992) Proc. Natl. Acad. Sci. USA 89, 12122-12126.
- Humphrey, M.B. et al. (1992) Gene 112, 197-204.
- Yamashiro, S. et al. (1991) Nature 349, 169-172.
- Mak, A.S. et al. (1991) J. Biol. Chem. 266, 6678-6681.
- Hai, C.M. and Gu, Z. (2006) Eur. J. Cell Biol. 85, 305-309.
- Yamashiro, S. et al. (2001) Mol. Biol. Cell 12, 239-250.
- Hedges, J.C. et al. (2000) Am. J. Physiol. Cell Physiol. 278, C718-C7126.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.