Cell Signaling Technology

Product Pathways - Development

Naked2 (C67C4) Rabbit mAb #2073

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 59, 61 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Naked2 (C67C4) Rabbit mAb detects endogenous level of Naked2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala217 of human Naked2.

Western blot analysis of extracts from various cell lines using Naked2 (C67C4) Rabbit mAb.

Background

Naked1 (Nkd1) and Naked2 (Nkd2) are homologs of Drosophila Naked cuticle, a negative regulator of Wnt/Wingless signaling pathway which functions through a feedback mechanism (1,2). Both Drosophila and vertebrate Naked proteins contain a putative calcium-binding EF-hand motif, however, Drosophila Naked binds to zinc instead of calcium (3). Naked inhibits the canonical Wnt/β-catenin pathway by binding to Dishevelled proteins and directs Dishevelled activity towards the planar cell polarity pathway (2,4). Naked1 is a direct target of Wnt signaling and is overexpressed in some colon tumors due to constitutive activation of Wnt/β-catenin pathway (5). Naked2 is myristoylated and is required for sorting of TGF-α to the basolateral plasma membrane of polarized epithelial cells (6).

  1. Zeng, W. et al. (2000) Nature 403, 789-795.
  2. Wharton, K.A. et al. (2001) Dev. Biol. 234, 93-106.
  3. Rousset, R. et al. (2002) J. Biol. Chem. 277, 49019-49026.
  4. Yan, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 3802-3807.
  5. Yan, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 14973-14978.
  6. Li, C. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 5571-5576.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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