Product Pathways - Tyrosine Kinase/ Adaptors
Non-phospho-Src (Tyr416) (7G9) Mouse mAb #2102
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP | H M R (C) (X) | 60 | Mouse | IgG2b |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
C=Chicken
X=Xenopus
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Non-phospho-Src (Tyr416) (7G9) Mouse mAb detects endogenous levels of Src only when dephosphorylated at tyrosine 416. The antibody cross-reacts with other Src family members (Lyn, Fyn, Lck, Yes and Hck) dephosphorylated at equivalent sites.
Source / Purification
Monoclonal antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Tyr416 of human Src.
Western Blotting
Western blot analysis of extracts from pervanadate-treated (1 mM for 5 minutes) NIH/3T3 cells that have been stably transfected with a constitutively active form of Src (in which the regulatory tyrosine 527 residue has been mutated to phenylalanine), A431 cells and HepG2 cells, using Non-phospho-Src (Tyr416) (7G9 ) Mouse mAb (top), v-Src antibody (middle) or Phospho-Src (Tyr416) Antibody #2101 (bottom).
Background
The Src family of protein tyrosine kinases (including Src, Lyn, Fyn, Yes, Lck, Blk and Hck) are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain by Csk upregulates enzyme activity, whereas phosphorylation of Tyr527 in the carboxy-terminal tail renders the enzyme less active (2).
- Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513-609.
- Hunter, T. (1987) Cell 49, 1-4.
Application References
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