Product Pathways - Tyrosine Kinase / Adaptors
Non-phospho-Src (Tyr416) (7G9) Mouse mAb #2102
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R (C) (X) | Endogenous | 60 | Mouse IgG2b |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
C=Chicken
X=Xenopus
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Non-phospho-Src (Tyr416) (7G9) Mouse mAb detects endogenous levels of Src only when dephosphorylated at tyrosine 416. The antibody cross-reacts with other Src family members (Lyn, Fyn, Lck, Yes and Hck) dephosphorylated at equivalent sites.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr416 of human Src.
Western Blotting
Western blot analysis of extracts from pervanadate-treated (1 mM for 5 minutes) NIH/3T3 cells that have been stably transfected with a constitutively active form of Src (in which the regulatory tyrosine 527 residue has been mutated to phenylalanine), A431 cells and HepG2 cells, using Non-phospho-Src (Tyr416) (7G9 ) Mouse mAb (top), v-Src antibody (middle) or Phospho-Src (Tyr416) Antibody #2101 (bottom).
Background
The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
- Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513-609.
- Hunter, T. (1987) Cell 49, 1-4.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2101 Phospho-Src Family (Tyr416) Antibody
- 2123 Src (32G6) Rabbit mAb
- 2105 Phospho-Src (Tyr527) Antibody
- 2107 Non-phospho-Src (Tyr527) Antibody
- 9935 Src Antibody Sampler Kit
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 2108 Src Antibody
- 2109 Src (36D10) Rabbit mAb
- 2110 Src (L4A1) Mouse mAb
For Research Use Only. Not For Use In Diagnostic Procedures.
