Cell Signaling Technology

Product Pathways - MAPK Signaling

p38γ MAP Kinase Antibody #2307

Applications Reactivity MW (kDa) Source
W IP H M R Mk 46 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

p38γ MAP Kinase Antibody detects endogenous levels of total p38γ MAP kinase protein. This antibody does not cross-react with the other p38 MAP kinase isoforms p38α, -β or -δ.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy-terminal residues of human p38γ MAP kinase. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of purified recombinant full-length p38 MAP kinase GST fusion protein, using p38 MAP kinase pan antibody (upper) or p38γ MAP Kinase Antibody (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from HUVEC and U937 cells, using p38γ MAP Kinase Antibody.

Background

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase which participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as ERK6 or SAPK3) and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3, MKK6 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).

Although there are many similarities between the four p38 isoforms, there are also some important differences that suggest that the various members may regulate specific functions, and the presence of multiple p38s may provide a mechanism for the generation of tissue-specific or stimulus-specific responses to the activation of the p38 signal transduction pathway (9,10).

  1. Rouse, J. et al. (1994) Cell 78, 1027-1037.
  2. Han, J. et al. (1994) Science 265, 808-811.
  3. Lee, J.C. et al. (1994) Nature 372, 739-746.
  4. Freshney, N.W. et al. (1994) Cell 78, 1039-1049.
  5. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
  6. Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.
  7. Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
  8. Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.
  9. Fearns, C. et al. (2000) J. Leukoc. Biol. 67, 705-711.
  10. Hale, K.K. et al. (1999) J. Immunol. 162, 4246-4252.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

Product Pathways

Drug Discovery Tools

Featured Technologies

Protein Classes