Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

ATRIP Antibody #2737

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC H Endogenous 82 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

ATRIP Antibody detects endogenous levels of total ATRIP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to amino acids surrounding Gly267 of human ATRIP. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and HEL cells using ATRIP Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using ATRIP Antibody (green). Actin filaments have been labeled with Alexa Fluor®555 phalloidin (red).

Background

In response to genomic stress, the ATR interacting protein (ATRIP) binds and is phosphorylated by the DNA damage-and checkpoint-activated kinase ATR (ataxia-telangiectasia mutated and rad3-related). Both ATR and ATRIP are integral for checkpoint signaling and are critical in the DNA repair response (1-3). Direct interaction between ATRIP and replication protein A (RPA) at RPA-coated, single-stranded DNA results in the recruitment of phosphorylated ATR/ATRIP to stalled replication forks and sites of DNA damage (3). ATR/ATRIP coordinate DNA repair and cell cycle progression in conjunction with key regulatory proteins, such as Rad17 and the 9-1-1 complex (4). ATR associated with ATRIP can also be stimulated by topoisomerase II binding protein (TOPBP1), suggesting that ATRIP may regulate both ATR localization and activity (5).

  1. Cortez, D. et al. (2001) Science 294, 1713-1716.
  2. Itakura, E. et al. (2004) Biochem. Biophys. Res. Commun. 323, 1197-1202.
  3. Zou, L. and Elledge, S.J. (2003) Science 300, 1542-1548.
  4. Yang, X.H. and Zou, L. (2006) Methods Enzymol. 409, 118-131.
  5. Ball, H.L. et al. (2007) Mol. Cell. Biol. [Epub ahead of print].

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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