Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Na,K-ATPase α1 (Tyr10) Antibody #3060

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M (R) (Mk) (B) (Pg) Endogenous 100 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Na,K-ATPase α1 (Tyr10) Antibody recognizes endogenous levels of Na,K-ATPase α1 only when phosphorylated at Tyr10. The antibody cross-reacts with an induced 75-80 kDa doublet of unknown origin.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr10 of rat Na,K-ATPase α1. Antibodies are purified using protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or vanadate-treated, and A431 cells, untreated or EGF-treated, using Phospho-Na,K-ATPase α1 (Tyr10) Antibody (upper) or total Na,K-ATPase α1 Antibody #3010 (lower).

Background

The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients. It is composed of a catalytic α subunit and a β subunit (reviewed in 1). Several phosphorylation sites have been identified for the α1 subunit. Tyr10 is phosphorylated by an as yet undetermined kinase (2), Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA (3-5). All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na,K-ATPase. Altered phosphorylation in response to angiotensin II stimulates activity in the rat proximal tubule (6). Na,K-ATPase is also involved in other signal transduction pathways. Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1/2 phosphorylation of the α subunit (7). Na,K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors (8,9).

  1. Therien, A.G. and Blostein, R. (2000) Am. J. Physiol. Cell Physiol. 279, C541-566.
  2. Féraille, E. et al. (1999) Mol. Biol. Cell 10, 2847-2859.
  3. Fisone, G. et al. (1994) J. Biol. Chem. 269, 9368-9373.
  4. Feschenko, M.S. and Sweadner, K.J. (1995) J. Biol. Chem. 270, 14072-14077.
  5. Beguin, P. et al. (1994) J. Biol. Chem. 269, 24437-24445.
  6. Yingst, D.R. et al. (2004) Am. J. Physiol. Renal Physiol. 287, F713-F721.
  7. Al-Khalili, L. et al. (2004) J. Biol. Chem. 279, 25211-25218.
  8. Tian, J. et al. (2006) Mol. Biol. Cell 17, 317-326.
  9. Liang, M. et al. (2006) J. Biol. Chem. 281, 19709-19719.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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