Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Pyk2 (Tyr402) Antibody #3291

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M (R) Endogenous 116 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Pyk2 (Tyr402) Antibody detects endogenous levels of Pyk2 only when phosphorylated at tyrosine 402. This antibody may cross-react with other phospho-tyrosine containing proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr402 of human Pyk2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Immunoprecipitation of Pyk2 from Ramos cells, untreated, H2O2-treated, or Pervanadate-treated, using Pyk2 Antibody #3292, followed by Western blot with Phospho-Pyk2 (Tyr402) Antibody (upper), or Pyk2 Antibody #3292 (lower).

Background

Protein tyrosine kinase Pyk2, also called CAKβ, RAFTK and CADTK, is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK) (1-4). Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G-protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration (5-7).

Pyk2 is tyrosine phosphorylated and activated upon ligation of TCR (8,9). Phosphorylated Tyr402 of Pyk2 is required for the phosphorylation of other tyrosines on Pyk2 and provides a binding site for Fyn SH2 in the T-cell activation (10).

  1. Avraham, S. et al. (1995) J. Biol. Chem. 270, 27742-27751.
  2. Lev, S. et al. (1995) Nature 376, 737-745.
  3. Sasaki, H. et al. (1995) J. Biol. Chem. 270, 21206-21219.
  4. Yu, H. et al. (1996) J. Biol. Chem. 271, 29993-29998.
  5. Duong, L. T. et al. (2001) J. Biol. Chem. 276, 7484-7492.
  6. Watson, J. M. et al. (2001) J. Biol. Chem. 276, 3536-3542.
  7. Tang, H. et al. (2002) J. Biol. Chem. 277, 5441-5447.
  8. Berg, N.N. and Ostergaard, H.L. (1997) J. Immunol. 159, 1753-1757.
  9. Ganju, R. K. et al. (1997) J. Exp. Med. 185, 1055-1063.
  10. Katagiri, T. et al. (2000) J. Biol. Chem. 275, 19645-19652.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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