Product Pathways - Tyrosine Kinase / Adaptors
Phospho-Pyk2 (Tyr402) Antibody #3291
|W IP||H M (R)||Endogenous||116||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-Pyk2 (Tyr402) Antibody detects endogenous levels of Pyk2 only when phosphorylated at tyrosine 402. This antibody may cross-react with other phospho-tyrosine containing proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr402 of human Pyk2. Antibodies are purified by protein A and peptide affinity chromatography.
Protein tyrosine kinase Pyk2, also called CAKβ, RAFTK and CADTK, is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK) (1-4). Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G-protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration (5-7).
Pyk2 is tyrosine phosphorylated and activated upon ligation of TCR (8,9). Phosphorylated Tyr402 of Pyk2 is required for the phosphorylation of other tyrosines on Pyk2 and provides a binding site for Fyn SH2 in the T-cell activation (10).
- Avraham, S. et al. (1995) J. Biol. Chem. 270, 27742-27751.
- Lev, S. et al. (1995) Nature 376, 737-745.
- Sasaki, H. et al. (1995) J. Biol. Chem. 270, 21206-21219.
- Yu, H. et al. (1996) J. Biol. Chem. 271, 29993-29998.
- Duong, L. T. et al. (2001) J. Biol. Chem. 276, 7484-7492.
- Watson, J. M. et al. (2001) J. Biol. Chem. 276, 3536-3542.
- Tang, H. et al. (2002) J. Biol. Chem. 277, 5441-5447.
- Berg, N.N. and Ostergaard, H.L. (1997) J. Immunol. 159, 1753-1757.
- Ganju, R. K. et al. (1997) J. Exp. Med. 185, 1055-1063.
- Katagiri, T. et al. (2000) J. Biol. Chem. 275, 19645-19652.
- McMullen, M. et al. (2004) Vascular endothelial growth factor-mediated activation of p38 is dependent upon Src and RAFTK/Pyk2. Oncogene 23, 1275-1282. Applications: Western Blotting
- Ho, O.H. et al. (2004) J Neurochem 91, 1344-57. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.