Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-Pyk2 (Tyr402) Antibody #3291
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M (R) | Endogenous | 116 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Pyk2 (Tyr402) Antibody detects endogenous levels of Pyk2 only when phosphorylated at tyrosine 402.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr402 of human Pyk2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Jurkat cells, untreated or treated with anti-CD3 and anti-CD28 antibodies (1 µg/ml and 3 µg/ml, respectively, for 10 minutes), using Phospho-Pyk2 (Tyr402) Antibody (upper) or Pyk2 Antibody #3292 (lower).
Background
Protein tyrosine kinase Pyk2, also called CAKbeta, RAFTK and CADTK, is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK) (1-4). Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G-protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration (5-7).
Pyk2 is tyrosine phosphorylated and activated upon ligation of TCR (8,9). Phosphorylated Tyr402 of Pyk2 is required for the phosphorylation of other tyrosines on Pyk2 and provides a binding site for Fyn SH2 in the T-cell activation (10).
- Avraham, S. et al. (1995) J. Biol. Chem. 270, 27742-27751.
- Lev, S. et al. (1995) Nature 376, 737-745.
- Sasaki, H. et al. (1995) J. Biol. Chem. 270, 21206-21219.
- Yu, H. et al. (1996) J. Biol. Chem. 271, 29993-29998.
- Duong, L. T. et al. (2001) J. Biol. Chem. 276, 7484-7492.
- Watson, J. M. et al. (2001) J. Biol. Chem. 276, 3536-3542.
- Tang, H. et al. (2002) J. Biol. Chem. 277, 5441-5447.
- Berg, N.N. and Ostergaard, H.L. (1997) J. Immunol. 159, 1753-1757.
- Ganju, R. K. et al. (1997) J. Exp. Med. 185, 1055-1063.
- Katagiri, T. et al. (2000) J. Biol. Chem. 275, 19645-19652.
Application References
- McMullen, M. et al. (2004) Vascular endothelial growth factor-mediated activation of p38 is dependent upon Src and RAFTK/Pyk2. Oncogene 23, 1275-1282. This article references the use of Phospho-Pyk2 (Tyr402) Antibody in the following applications: Western Blotting
- Ho, O.H. et al. (2004) J Neurochem 91, 1344-57. This article references the use of Phospho-Pyk2 (Tyr402) Antibody in the following applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3292 Pyk2 Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
