Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Phospho-BLNK (Tyr96) Antibody #3601

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 68, 70 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-BLNK (Tyr96) Antibody detects endogenous levels of BLNK only when phosphorylated at tyrosine 96.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Tyr96 of human BLNK. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of SDS extracts from control or anti-human IgM treated (12 µg/ml for 2 minutes) Ramos cells using Phospho-BLNK (Tyr96) Antibody.

Background

B cell linker protein (BLNK), also known as SLP-65 or BASH, is an adaptor molecule that plays key roles in B cell activation and B cell antigen receptor (BCR) engagement. BLNK acts at the interface between BCR-associated Syk and downstream signaling cascades (1,2). BLNK has multiple SH2 binding motifs (YXXP) at its amino terminus and an SH2 domain at its carboxy terminus. After BCR ligation, BLNK is phosphorylated by Syk at multiple YXXP motifs including Tyr72, Tyr84, Tyr96, and Tyr178 (1). These phosphorylated motifs provide docking sites for signaling molecules, such as Btk, PLCγ, and Vav. These signaling molecules bind to BLNK through their SH2 domains and together activate downstream signaling pathways (3,4). Through its SH2 domain, BLNK can also interact with tyrosine-phosphorylated targets, such as HPK1, thereby recruiting them to the BCR complex for signaling (5).

  1. Kurosaki, T. and Tsukada, S. (2000) Immunity 12, 1-5.
  2. Fu, C. et al. (1998) Immunity 9, 93-103.
  3. Ishiai, M. et al. (1999) Immunity 10, 117-125.
  4. Baba, Y. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 2582-2586.
  5. Tsuji, S. et al. (2001) J. Exp. Med. 194, 529-539.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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