Product Pathways - Adhesion
Integrin beta-5 (D24A5) Rabbit mAb #3629
|3629S||100 µl (10 western blots)||---||In Stock||---|
|3629||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||90||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Integrin beta-5 (D24A5) Rabbit mAb detects endogenous levels of total integrin beta-5 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys789 of human integrin beta-5 protein.
Western blot analysis of extracts from various cell lines using Integrin beta-5 (D24A5) Rabbit mAb.
Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins having distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with extracellular environment (inside-out signaling) (1,2).
The αVβ5 integrin is expressed in various tissues and cell types, including endothelia, epithelia and fibroblasts (4). It plays a role in matrix adhesion to VN, FN, SPARC and bone sialoprotein (5) and functions in the invasion of gliomas and metastatic carcinoma cells (6,7). αVβ5 integrin plays a major role in growth-factor-induced tumor angiogenesis, where cooperative signaling by the αVβ5 integrin and growth factors regulates endothelial cell proliferation and survival (8).
- Hood, J.D. and Cheresh, D.A. (2002) Nat. Rev. Cancer 2, 91-100.
- Liu, S. et al. (2000) J. Cell Sci. 113, 3563-3571.
- van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res. 305, 285-298.
- Pasqualini, R. et al. (1993) J. Cell Sci. 105, 101-111.
- Plow, E.F. et al. (2000) J. Biol. Chem. 275, 21785-21788.
- Sun, L.C. et al. (2007) Cancer Lett. 246, 157-166.
- Enns, A. et al. (2005) Eur. J. Cancer 41, 1065-1072.
- Alghisi, G.C. and Rüegg, C. (2006) Endothelium 13, 113-135.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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