Cell Signaling Technology

Product Pathways - Neuroscience

Neuropilin-1 (D62C6) Rabbit mAb #3725

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M Endogenous 120, 80 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Neuropilin-1 (D39A5) Rabbit mAb detects endogenous levels of total neuropilin-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST-fusion protein corresponding to residues of mouse neuropilin-1.

Western Blotting

Western Blotting

Western blot analysis of extracts from U-87 MG and PC3 cells using Neuropilin-1 (D62C6) Rabbit mAb.

Background

Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single pass, transmembrane signaling proteins encompassing Plexin A1, A2, A3 and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3).

Neuropilin-1, a single pass transmembrane glycoprotein, was originally identified as a semaphorin receptor mediating axon growth cone collapse. In addition, neuropilin-1 is involved in axonal fasciculation, neuronal migration, dendritic guidance and repair of the adult nervous system (4). Neuropilin-1 is also an isoform-specific VEGF receptor on tumor and endothelial cells. Loss of neuropilin-1 function causes vascular remodeling and branching defects, which is compounded by additional loss of neuropilin-2 function (4, 5).

  1. Pasterkamp, R.J. and Kolodkin, A.L. (2003) Curr Opin Neurobiol 13, 79-89.
  2. Takahashi, T. and Strittmatter, S.M. (2001) Neuron 29, 429-39.
  3. Zanata, S.M. et al. (2002) J Neurosci 22, 471-7.
  4. He, Z. et al. (2002) Sci STKE 2002, RE1.
  5. Kawasaki, T. et al. (1999) Development 126, 4895-902.
  6. Takashima, S. et al. (2002) Proc Natl Acad Sci USA 99, 3657-62.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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