Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Jak2 (Tyr1007/1008) Antibody #3771

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R (B) Endogenous 125 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

Phospho-Jak2 (Tyr1007/1008) Antibody detects endogenous levels of Jak2 only when phosphorylated at Tyr1007/1008. This antibody may cross-react with phospho-Jak1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1007/1008 of human Jak2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from TF-1 cells, starved for 18 hours and treated with GM-CSF (25 ng/ml for 10 minutes), using Phospho-Jak2 (Tyr1007/1008) Antibody (upper) or control Jak2 antibody (lower).

Background

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

The tyrosine residues 1007/1008 of Jak2 in the putative activation loop are the homologous tyrosine residues 1054/1055 in Tyk2, which are important in the regulation of Tyk2 kinase activity (6).

  1. Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
  2. Darnell, J.E. (1997) Science 277, 1630-1635.
  3. VanderKuur, J. et al. (1995) J. Biol. Chem. 270, 7587-7593.
  4. Argetsinger, L.S. et al. (1995) J. Biol. Chem. 270, 14685-14692.
  5. Zhu, T. et al. (1998) J. Biol. Chem. 273, 10682-10689.
  6. Gauzzi, M.C. et al. (1996) J. Biol. Chem. 271, 20494-20500.

Application References

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Companion Products

This product is sold under license from Chemicon, Inc., U.S. Patent No. 5,658,791.

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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