Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Chromatin Regulation / Epigenetics

PHF20 (D96F6) XP® Rabbit mAb #3934

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M R Mk (B) (Hr) Endogenous 150 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine  Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PHF20 (D96F6) XP® Rabbit mAb detects endogenous levels of total PHF20 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the human PHF20 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HCT116 and F9 cells using PHF20 (D96F6) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cells using PHF20 (D96F6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Background

PHD finger protein 20 (PHF20) is a putative transcription factor protein. PHF20 contains a tudor domain, which facilitates binding to di-methylated histone H4 Lys20 (1). PHF20 may contribute to the development of cancers, including glioblastoma, lung cancer, colon cancer and ovarian cancer (2-5).

  1. Kim, J. et al. (2006) EMBO Rep 7, 397-403.
  2. Fischer, U. et al. (2001) Clin Exp Immunol 126, 206-13.
  3. Taniwaki, M. et al. (2006) Int J Oncol 29, 567-75.
  4. Bankovic, J. et al. (2009) Lung Cancer 67, 151-9 .
  5. Wang, Y. et al. (2002) J Immunol 169, 1102-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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