Product Pathways - Glucose Metabolism
HSL Antibody #4107
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC | M (H) | Endogenous | 81,83 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
HSL Antibody detects endogenous levels of total HSL protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human HSL. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NIH/3T3 and 3T3-L1 cells using HSL Antibody.
IF-IC
Confocal immunofluorescent analysis of 3T3-L1 adipocytes using HSL Antibody (red). Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
HSL (hormone-sensitive lipase) catalyzes the hydrolysis of triacylglycerol, the rate-limiting step in lipolysis. Lipolytic stimuli activate adenylyl cyclase and thus increase intracellular cAMP levels, which in turn activate protein kinase A (PKA). PKA phosphorylates HSL at Ser563, Ser659 and Ser660, which stimulates HSL activity (1,2). In contrast, AMPK phosphorylates HSL at Ser565, which reduces HSL phosphorylation at Ser563 by PKA and inhibits HSL activity (2,3). Recent work indicates that phosphorylation at Ser600 by p44/42 MAPKs also enhances the enzymatic activity of HSL (4).
- Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533-537.
- Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215-221.
- Garton, A.J. and Yeaman, S.J. (1990) Eur. J. Biochem. 191, 245-250.
- Greenberg, A.S. et al. (2001) J. Biol. Chem. 276, 45456-45461.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
