Product Pathways - Adhesion
Ajuba Antibody #4897
PhosphoSitePlus® protein, site, and accession data: JUB
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP F | H M Mk B (R) | Endogenous | 55 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4897:
- Flow, Immunoprecipitation, Western Blotting
Specificity / Sensitivity
Ajuba Antibody detects endogenous levels of total Ajuba protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Ajuba. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from MEF -/- cells rescued with a construct overexpressing Ajuba (lane 1), MEF -/- cells (lane 2), MEF +/+ cells (lane 3) and P19 cells (lane 4), using Ajuba Antibody. (Kindly provided by Dr. Gregory D. Longmore, Washington University, St. Louis, Missouri.)
Western Blotting
Western blot analysis of extracts from P19 cells (lane 1) and COS-7 cells transfected with Ajuba (lane 2), using Ajuba Antibody.
Flow Cytometry
Flow cytometric analysis of COS cells, using Ajuba antibody (blue) compared to a non-specifc negative control antibody (red).
Background
Ajuba belongs to the Zyxin/Ajuba family, members of which include LIMD1, LPP, Trip6 and Zyxin. Three LIM domains at the carboxy-terminus characterize this family of proteins. Zyxin/Ajuba family members associate with the actin cytoskeleton, are components of both cell-cell junction adhesive complex and integrin-mediated adhesive complex, and shuttle in and out of the nucleus (1). Ajuba is involved in Rac activation during cell migration and activates Aurora A in order to commit to mitosis (2-3). Ajuba and its family members also have an amino-terminal proline-rich pre-LIM region, and Ajuba augments ERK activation in a Ras-dependent manner (4).
- Feng, Y. and Longmore, G.D. (2005) Mol. Cell Biol. 25, 4010-4022.
- Pratt, S. J. et al. (2005) J. Cell Biol. 168, 813-824.
- Hirota, T. et al. (2003) Cell 114, 585-598.
- Goyal, R. et al. (1999) Mol. Cell Biol. 19, 4379-4389.
Application References
- Montoya-Durango, D.E. et al. (2008) J Biol Chem 283, 32056-65. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.
