Cell Signaling Technology

Product Pathways - Metabolism

Fetuin A (F180) Antibody #5258

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 45-50 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Fetuin A (F180) Antibody detects endogenous levels of total fetuin A protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe180 of human fetuin A protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of human serum using Fetuin A (F180) Antibody. Human serum (0.1 μl) was analyzed.

Background

Fetuin A is produced by liver and secreted to plasma, and selectively concentrated in bone matrix. It is synthesized as a precursor and processed to generate 2 chains that are held together by a disulfide bond. It is a systemically acting calcification inhibitor (1). Reduced level of fetuin A in serum is associated with increased cardiovascular mortality in dialysis patients (2,3). Fetuin A can inhibit insulin-induced tyrosine phosphorylation of the insulin receptor tyrosine kinase and insulin receptor substrate-1 (4). Fetuin A deficient mice show high insulin sensitivity and high levels of serum fetuin A are associated with insulin resistance in humans (5,6).

  1. Schafer, C. et al. (2003) J Clin Invest 112, 357-66.
  2. Westenfeld, R. et al. (2007) Trends Cardiovasc Med 17, 124-8.
  3. Westenfeld, R. et al. (2009) J Am Soc Nephrol 20, 1264-74.
  4. Auberger, P. et al. (1989) Cell 58, 631-40.
  5. Mathews, S.T. et al. (2002) Diabetes 51, 2450-8.
  6. Stefan, N. et al. (2006) Diabetes Care 29, 853-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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