Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

JMJD1B (6A1-1F5) Mouse mAb #5377

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M R Mk Endogenous 220 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

JMJD1B (6A1-1F5) Mouse mAb detects endogenous levels of total JMJD1B protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to the sequence of human JMJD1B.

Western Blotting

Western Blotting

Western blot analysis of extracts from NCCIT cells using JMJD1B (6A1-1F5) Mouse mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using JMJD1B (6A1-1F5) Mouse mAb (green). Actin filaments were labeled using DY-554 phalloidin (red).

Background

The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain of several proteins has been shown to catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into seven separate families (3). The JMJD1 (Jumonji domain-containing protein 1) family, also known as JHDM2 (JmjC domain-containing histone demethylation protein 2) family, contains four members: hairless (HR), JMJD1A/JHDM2A, JMJD1B/JHDM2B, and JMJD1C/JHDM2C. Hairless is expressed in the skin and brain and acts as a co-repressor of the thyroid hormone receptor (4-6). Mutations in the hairless gene cause alopecia in both mice and humans (4,5). JMJD1A is expressed in meiotic and post-meiotic male germ cells, contributes to androgen receptor-mediated gene regulation, and is required for spermatogenesis (7-9). It has also been identified as a downstream target of OCT4 and STAT3 and is critical for the regulation of self-renewal in embryonic stem cells (10,11). JMJD1B is a more widely expressed family member and is frequently deleted in myeloid leukemia (12). JMJD1C (also known as TRIP8) is a co-factor of both the androgen and thyroid receptors and has a potential link to autism (13-15). Members of the JMJD1/JHDM2 family have been shown to demethylate mono-methyl and di-methyl histone H3 (Lys9) (3,8).

  1. Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop , 1-27.
  2. Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42.
  3. Klose, R.J. et al. (2006) Nat Rev Genet 7, 715-27.
  4. Cachon-Gonzalez, M.B. et al. (1994) Proc Natl Acad Sci USA 91, 7717-21.
  5. Ahmad, W. et al. (1998) Science 279, 720-4.
  6. Potter, G.B. et al. (2001) Genes Dev 15, 2687-701.
  7. Höög, C. et al. (1991) Mol Reprod Dev 30, 173-81.
  8. Yamane, K. et al. (2006) Cell 125, 483-95.
  9. Okada, Y. et al. (2007) Nature 450, 119-23.
  10. Loh, Y.H. et al. (2007) Genes Dev 21, 2545-57.
  11. Ko, S.Y. et al. (2006) Cell Struct Funct 31, 53-62.
  12. Hu, Z. et al. (2001) Oncogene 20, 6946-54.
  13. Lee, J.W. et al. (1995) Mol Endocrinol 9, 243-54.
  14. Wolf, S.S. et al. (2007) Arch Biochem Biophys 460, 56-66.
  15. Castermans, D. et al. (2007) Eur J Hum Genet 15, 422-31.

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