Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

BCL6 (D65C10) Rabbit mAb #5650

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M Endogenous 87-98 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

BCL6 (D65C10) Rabbit mAb recognizes endogenous levels of total BCL6 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to human BCL6 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using BCL6 (D65C10) Rabbit mAb.

Background

Chromosomal translocations result in misregulation of the proto-oncogene BCL6 in patients with B cell-derived non-Hodgkins lymphoma (1). The BCL6 gene is selectively expressed in mature B cells and encodes a nuclear phosphoprotein that belongs to the BTB/POZ zinc finger family of transcription factors (2,3). BCL6 protein can bind to target DNA sequences of Stat6 and, analogous to Stat6, modulate the expression of interleukin-4-induced genes (4). Furthermore, BCL6 restrains p53-dependent senescence, making BCL6-active tumors functionally p53-negative (5). The mitogen-activated protein kinases, Erk1 and Erk2, but not JNK, phosphorylate BCL6 at multiple sites. Phosphorylation of BCL6 at Ser333 and Ser343 results in degradation of BCL6 by the ubiquitin/proteasome pathway in B cells (6,7). In addition, BCL6 is acetylated and its transcriptional repressor function is inhibited by the transcriptional co-activator p300 (8).

  1. Ye, B. H. et al. (1993) Science 262, 747-750.
  2. Onizuka, T. et al. (1995) Blood 86, 28-37.
  3. Zollman, S. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 10717-10721.
  4. Harris, M. B. et al. (1999) Mol. Cell. Biol. 19, 7264-7275.
  5. Shvarts, A. et al. (2002) Genes Dev. 16, 681-686.
  6. Moriyama, M. et al. (1997) Oncogene 14, 2465-2474.
  7. Niu, H. et al. (1998) Genes Dev. 12, 1953-1961.
  8. Bereshchenko, O. R. et al. (2002) Nat. Genet. 32, 606-613.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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