Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

ApoA4 (1D6B6) Mouse mAb #5700

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 45 Mouse IgG2a

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ApoA4 (1D6B6) Mouse mAb recognizes endogenous levels of total ApoA4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human ApoA4 protein.

Western Blotting

Western Blotting

Western blot analysis of Hep G2 cell extracts and human plasma using ApoA4 (1D6B6) Mouse mAb.

Background

Apolipoproteins are plasma lipoproteins that function as transporters of lipids and cholesterol in the circulatory system. Chylomicrons are a fundamental class of apolipoproteins containing very low-density lipoproteins (VLDL), intermediate-density lipoproteins (IDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) (1,2).

Apolipoprotein A-IV (ApoA4) is a glycoprotein that exists as a free apolipoprotein in human plasma (3,4). ApoA4 activates the lecithin cholesterol acyl transferase (5-7) to modulate the rate of lipid transfer protein (LTP) activity (7). Additional evidence suggests that ApoA4 may also act as an antioxidant to inhibit LDL oxidation in the atherosclerosis model (8).

  1. Kwiterovich, P.O. (2000) Am J Cardiol 86, 5L-10L.
  2. Hussain, M.M. (2000) Atherosclerosis 148, 1-15.
  3. Utermann, G. and Beisiegel, U. (1979) Eur J Biochem 99, 333-43.
  4. Hayashi, H. et al. (1990) J Lipid Res 31, 1613-25.
  5. Steinmetz, A. and Utermann, G. (1985) J Biol Chem 260, 2258-64.
  6. Chen, C.H. and Albers, J.J. (1985) Biochim Biophys Acta 836, 279-85.
  7. Main, L.A. et al. (1996) Biochim Biophys Acta 1300, 17-24.
  8. Wong, W.M. et al. (2007) Atherosclerosis 192, 266-74.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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