Product Pathways - Ca / cAMP / Lipid Signaling
ApoM (8F12) Mouse mAb #5709
PhosphoSitePlus® protein, site, and accession data: ApoM
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H (M) (R) | Endogenous | 21, 25 | Mouse IgG2a |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
ApoM (8F12) Mouse mAb recognizes endogenous levels of both unglycosylated and glycosylated ApoM protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a purified recombinant fragment of human ApoM protein.
Western Blotting
Western blot analysis of Hep G2 cell extracts and human plasma using ApoM (8F12) Mouse mAb.
Background
Apolipoproteins are plasma lipoproteins that function as transporters of lipids and cholesterol in the circulatory system. Chylomicrons are a fundamental class of apolipoproteins containing very low-density lipoproteins (VLDL), intermediate-density lipoproteins (IDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) (1,2).
Apolipoprotein M (ApoM) is a novel membrane bound apolipoprotein involved in lipid transport. ApoM primarily interacts with HDL and regulates its metabolism (3). It is highly expressed in hepatocytes and kidney proximal tubule cells. Recently, ApoM was shown to act as a vasculoprotective constituent of HDL by delivering S1P to the S1P receptor on endothelial cells (4). SNPs of ApoM have been associated with type 2 diabetes (5).
- Kwiterovich, P.O. (2000) Am J Cardiol 86, 5L-10L.
- Hussain, M.M. (2000) Atherosclerosis 148, 1-15.
- Xu, N. and Dahlbäck, B. (1999) J Biol Chem 274, 31286-90.
- Christoffersen, C. et al. (2011) Proc Natl Acad Sci U S A 108, 9613-8.
- Zhou, J.W. et al. (2011) PLoS One 6, e17324.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
