Cell Signaling Technology

Product Pathways - Development

Axin2 (D48G4) Rabbit mAb #5863

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 95, 98 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Axin2 (D48G4) Rabbit mAb detects endogenous levels of total Axin2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro566 of human Axin2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HCT-15 and SW620 cells using Axin2 (D48G4) Rabbit mAb.

Background

Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 interacts with APC, GSK-3β, Dvl, and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice (6). Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9).

  1. Luo, W. and Lin, S.C. Neurosignals 13, 99-113.
  2. Salahshor, S. and Woodgett, J.R. (2005) J. Clin. Pathol. 58, 225-236.
  3. Zeng, X. et al. (2005) Nature 438, 873-877.
  4. Davidson, G. et al. (2005) Nature 438, 867-872.
  5. Zhang, Y. et al. (1999) J. Biol. Chem. 274, 35247-35254.
  6. Chia, I.V. and Costantini, F. (2005) Mol. Cell Biol. 25, 4371-4376.
  7. Jho, E.H. et al. (2002) Mol. Cell Biol. 22, 1172-1183.
  8. Lustig, B. et al. (2002) Mol. Cell Biol. 22, 1184-1193.
  9. Leung, J.Y. et al. (2002) J. Biol. Chem. 277, 21657-21665.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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