Product Pathways - Translational Control
Miwi (D92B7) XP® Rabbit mAb #6915
PhosphoSitePlus® protein, site, and accession data: PIWIL1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-F | M (H) (R) | Endogenous | 90 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Miwi (D92B7) XP® Rabbit mAb recognizes endogenous levels of total Miwi protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly81 of mouse Miwi protein.
Western Blotting
Western blot analysis of extracts from mouse testis and mouse brain using Miwi (D92B7) XP® Rabbit mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower).
IF-F
Confocal immunofluorescent analysis of mouse testis using Miwi (D92B7) XP® Rabbit mAb. Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
The Drosophila piwi gene was identified as being required for the self-renewal of germline stem cells (1). Piwi homologs are well conserved among various species including Arabidopsis, C. elegans, and Homo sapiens (1). Both Miwi and Mili proteins are mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mice (3-5).
- Cox, D.N. et al. (1998) Genes Dev 12, 3715-27.
- Kuramochi-Miyagawa, S. et al. (2001) Mech Dev 108, 121-33.
- Aravin, A. et al. (2006) Nature 442, 203-7.
- Grivna, S.T. et al. (2006) Proc Natl Acad Sci USA 103, 13415-20.
- Grivna, S.T. et al. (2006) Genes Dev 20, 1709-14.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
