Product Pathways - Neuroscience
NCS1 (D12D2) XP® Rabbit mAb #8237
PhosphoSitePlus® protein, site, and accession data: FREQ
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-IC | H M R Hm Mk | Endogenous | 21 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Hm=Hamster
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
NCS1 (D12D2) XP® Rabbit mAb recognizes endogenous levels of total NCS1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human NCS1 protein.
Western Blotting
Western blot analysis of extracts from mouse brain, rat brain, and human cerebellum using NCS1 (D12D2) XP® Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of COS-7 cells using NCS1 (D12D2) XP® Rabbit mAb (green). Red = Propidium Iodide/RNase (fluorescent DNA dye).
Background
According to affinity and function, calcium-binding proteins are separated into two classes: calcium buffers and calcium sensors. Calmodulin is a well-studied calcium sensor with well-established roles in synaptic plasticity. Neuronal calcium-sensor 1 (NCS1) is also a member of the calcium sensor family, however, its role in synaptic plasticity remains under investigation. NCS1 contains multiple EF-hand calcium-binding motifs and an amino-terminal myristoyl group (1). NCS1 has a large number of binding partners. Most of these protein interactions are calcium-dependent (e.g. dopamine D2 receptor), although some are calcium-independent (e.g. IP3 receptor) (2). In murine dentate gyrus, NCS1 promotes synaptic plasticity and rapid acquisition of spatial memory (3).
- Amici, M. et al. (2009) Biochem Soc Trans 37, 1359-63.
- Burgoyne, R.D. (2007) Nat Rev Neurosci 8, 182-93.
- Saab, B.J. et al. (2009) Neuron 63, 643-56.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
