Product Pathways - Cell Cycle / Checkpoint
cdc2 (POH1) Mouse mAb #9116
|9116S||100 µl (20 western blots)||---||In Stock||---|
|9116||carrier free and custom formulation / quantity||email request|
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|W||1:2000||Human, Monkey||Endogenous||34||Mouse IgG2a|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
cdc2 (POH1) Mouse mAb detects endogenous levels of total cdc2 protein. The antibody does not cross-react with other cdks.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant human cdc2 fusion protein.
Western blot analysis of extracts from HeLa cells synchronized at various stages of the cell cycle, using cdc2 (POH1) Mouse mAb.
The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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