Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

cdc2 (POH1) Mouse mAb #9116

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-IC H Mk Endogenous 34 Mouse IgG2a

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

cdc2 (POH1) Mouse mAb detects endogenous levels of total cdc2 protein. The antibody does not cross-react with other cdks.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human cdc2 fusion protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells synchronized at various stages of the cell cycle, using cdc2 (POH1) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and slight cytoplasmic localization, using cdc2 (POH1) Mouse mAb.

IF-IC

IF-IC

Immunofluorescent analysis of proliferating HeLa cells, using cdc2 (POH1) Mouse mAb.


Background

The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).

  1. Atherton-Fessler, S. et al. (1994) Mol. Biol. Cell. 5, 989-1001.
  2. Norbury, C. et al. (1991) EMBO. J. 10, 3321-3329.
  3. McGowan, C.H. and Russell, P. (1993) EMBO J. 12, 75-85.
  4. Wells, N.J. et al. (1999) J. Cell. Sci. 112, 3361-3371.
  5. Hunter, T. (1995) Cell 80, 225-236.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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