Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

Slug (C19G7) Rabbit mAb #9585

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-IC H M Endogenous 30 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Slug (C19G7) Rabbit mAb detects endogenous levels of total Slug protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human Slug protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from A204, SKMEL5, and NIH/3T3 cells using Slug (C19G7) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Slug (C19G7) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded A204 cells (positive) (left) and HCT15 cells (negative) (right) using Slug (C19G7) Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of A204 cells (left) and PANC-1 cells (right) using Slug (C19G7) Rabbit mAb (green). Actin filaments have been labeled with DY554 phalloidin (red).

Background

Slug (SNAI2) is a widely expressed transcriptional repressor and member of the Snail family of zinc finger transcription factors (1). Similar to the related Snail protein, Slug binds to the E-cadherin promoter region to repress transcription during development (2). The binding of Slug to integrin promoter sequences represses integrin expression and results in reduced cell adhesion (3). Down regulation of E-cadherin expression occurs during the epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells (4,5). The tumor suppressor protein p53 induces Slug expression in γ-irradiated cells; Slug protects damaged cells from apoptosis by repressing p53-induced transcription of the proapoptotic Bcl-2 family protein Puma (6). Deletion mutations in the corresponding Slug gene are associated with the pigmentation disorders Waardenburg Syndrome and Piebaldism, while a genetic duplication resulting in Slug overexpression is associated with a collection of congenital heart defects termed tetralogy of Fallot (7).

  1. Inukai, T. et al. (1999) Mol Cell 4, 343-52.
  2. Bolos, V. et al. (2003) J Cell Sci 116, 499-511.
  3. Turner, F.E. et al. (2006) J Biol Chem 281, 21321-31.
  4. Barrallo-Gimeno, A. and Nieto, M.A. (2005) Development 132, 3151-61.
  5. Castro Alves, C. et al. (2007) J Pathol 211, 507-15.
  6. Wu, W.S. et al. (2005) Cell 123, 641-53.
  7. Perez-Mancera, P.A. et al. (2006) Cytogenet Genome Res 114, 24-9.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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