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H Endogenous 59, 61 Rabbit 

Western blot analysis of extracts from various cell lines using Naked2 (C67C4) Rabbit mAb.

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Product Usage Information

Application Dilutions
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Naked2 (C67C4) Rabbit mAb detects endogenous level of Naked2 protein.

Species Reactivity: Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala217 of human Naked2.

Naked1 (Nkd1) and Naked2 (Nkd2) are homologs of Drosophila Naked cuticle, a negative regulator of Wnt/Wingless signaling pathway which functions through a feedback mechanism (1,2). Both Drosophila and vertebrate Naked proteins contain a putative calcium-binding EF-hand motif, however, Drosophila Naked binds to zinc instead of calcium (3). Naked inhibits the canonical Wnt/β-catenin pathway by binding to Dishevelled proteins and directs Dishevelled activity towards the planar cell polarity pathway (2,4). Naked1 is a direct target of Wnt signaling and is overexpressed in some colon tumors due to constitutive activation of Wnt/β-catenin pathway (5). Naked2 is myristoylated and is required for sorting of TGF-α to the basolateral plasma membrane of polarized epithelial cells (6).

1.  Zeng, W. et al. (2000) Nature 403, 789-795.

2.  Rousset, R. et al. (2002) J. Biol. Chem. 277, 49019-49026.

3.  Yan, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 14973-14978.

4.  Li, C. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 5571-5576.

5.  Wharton, K.A. et al. (2001) Dev Biol 234, 93-106.

6.  Yan, D. et al. (2001) Proc Natl Acad Sci U S A 98, 3802-7.

Entrez-Gene Id 85409
Swiss-Prot Acc. Q969F2

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.