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M R Endogenous 165 Rabbit

Western blot analysis of extracts from the indicated rat brain synaptic fractions using SHANK2 Antibody. Separation of the different synaptic fractions was confirmed using PSD95 (D27E11) XP® Rabbit mAb #3450 and Syntaxin 6 (C34B2) Rabbit mAb #2869. Equal loading of each fraction was assessed using β-Tubulin (9F3) Rabbit mAb #2128. Fractionation of the different synaptic compartments was carried out as described by Phillips, G.R. et al. (2001) Neuron 32, 63-77. PAZ, Pre-synaptic active zone.

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Western blot analysis of extracts from mouse and rat brain tissue using SHANK2 Antibody.

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Product Usage Information

Application Dilutions
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

SHANK2 Antibody recognizes endogenous levels of total SHANK2 protein.

Species Reactivity: Mouse, Rat
Species predicted to react based on 100% sequence homology: Human, Monkey, Bovine

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val537 of human SHANK2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

The SHANK family proteins, also known as proline-rich synapse-associated proteins, consist of SHANK1, SHANK2, and SHANK3. SHANK proteins act as scaffolds at the neuronal post-synaptic density (PSD) (1), where they play a critical role in PSD assembly of excitatory synapses during development (2). While recruitment of SHANK proteins to the synapse is independent of their interaction with Homer (3), proper synaptic targeting of SHANK1 is mediated by interactions between its PDZ domain and PSD proteins (4). At the synapse, SHANK proteins interact with NMDA receptors and metabotropic glutamate receptor complexes (5). Research studies have proposed the involvement of SHANK proteins in autism and neurodegenerative diseases (6).

1.  Grabrucker, A.M. et al. (2011) Trends Cell Biol 21, 594-603.

2.  Grabrucker, A.M. et al. (2011) Trends Cell Biol 21, 594-603.

3.  Boeckers, T.M. et al. (1999) J Neurosci 19, 6506-18.

4.  Boeckers, T.M. et al. (2005) J Neurochem 92, 519-24.

5.  Boeckers, T.M. et al. (2002) J Neurochem 81, 903-10.

6.  Sala, C. et al. (2001) Neuron 31, 115-30.

Entrez-Gene Id 22941
Swiss-Prot Acc. Q9UPX8-4

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SHANK2 Antibody