News from the Bench

Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.

Subscribe

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

To Purchase # 5946S

5946S 1 ml (40 immunoprecipitations) $129.00
$ 0. 00

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Image

Immunoprecipitation of COS-7 cell extracts, untransfected or transfected with a construct overexpressing TrCP1-GST using GST (26H1) Mouse mAb #2624. Extracts were incubated with either Anti-Mouse IgG (H+L), F(ab')2 Fragment (Sepharose® Bead Conjugate) (lane 1 and 2) or Protein G Beads (lane 3). The western blot was probed using GST (91G1) Rabbit mAb #2625.

Learn more about how we get our images

Immunoprecipitation of NIH/3T3 cell extracts using Akt (pan)(40D4) Mouse mAb #2920. Extracts were incubated with either Anti-Mouse IgG (H+L), F(ab')2 Fragment (Sepharose® Bead Conjugate) (lane 1) or Protein G Beads (lane 2). The western blot was probed using Akt (pan) (C67E7) Rabbit mAb #4691.

Learn more about how we get our images
Image
Image
Image
Image
Image

Product Usage Information

Add 10-20 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.


Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Product Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Anti-mouse IgG (H+L), F(ab')2 Fragment (Sepharose Bead Conjugate) is useful for the immunoprecipitation of antibodies raised in mice.


Specificity / Sensitivity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, mouse IgG and mouse serum. No reaction was observed against anti-pepsin, anti-goat IgG Fc, or human serum proteins.


Species Reactivity: Mouse

Source / Purification

The Anti-mouse IgG (H+L), F(ab')2 Fragment is produced by immunizing goats with mouse IgG whole molecules. The Anti-mouse IgG (H+L), F(ab')2 Fragment is prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities, pepsin digestion, and chromatographic separation.

Anti-mouse IgG (H+L), F(ab')2 Fragment (Sepharose Bead Conjugate) is ideal for immunoprecipitation. This secondary antibody will detect mouse antibodies. Since this secondary antibody is made with F(ab')2 fragments, non-specific binding through Fc receptors present on cells will be eliminated.



For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.