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Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (Sepharose® Bead Conjugate) #4088
This product is discontinued
Gallery: Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (Sepharose® Bead Conjugate) #4088
Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (Sepharose® Bead Conjugate) detects endogenous levels of IκBα only when phosphorylated at Ser32/36.Species predicted to react based on 100% sequence homology: Bovine, Dog, Pig, Guinea Pig
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser32/36 of human IκBα.
This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose® beads. Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (Sepharose® Bead Conjugate) is useful for the immunoprecipitation of phosphorylated IκBα.
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
Protein Specific References
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.