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150 Rabbit 

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Specificity / Sensitivity

Raptor (24C12) Rabbit mAb (Sepharose® Bead Conjugate) detects endogenous levels of total Raptor protein.

Source / Purification

Raptor (24C12) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Raptor.

Product Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose® beads. Raptor (24C12) Rabbit mAb (Sepharose® Bead Conjugate) is useful for immunoprecipitation assays. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Raptor (24C12) Rabbit mAb #2280.

The regulatory associated protein of mTOR (Raptor) was identified as an mTOR binding partner that mediates mTOR signaling to downstream targets (1,2). Raptor binds to mTOR substrates, including 4E-BP1 and p70 S6 kinase, through their TOR signaling (TOS) motifs and is required for mTOR-mediated phosphorylation of these substrates (3,4). Binding of the FKBP12-rapamycin complex to mTOR inhibits the mTOR-raptor interaction, suggesting a mechanism for rapamycin's specific inhibition of mTOR signaling (5). This mTOR-raptor interaction and its regulation by nutrients and/or rapamycin is dependent on a protein called GβL (6). GβL is also part of the rapamycin-insensitive complex between mTOR and rictor (rapamycin-insensitive companion of mTOR), and may mediate rictor-mTOR signaling to downstream targets including PKCα (7). Furthermore, the rictor-mTOR complex has been identified as the previously elusive PDK2 responsible for the phosphorylation of Akt/PKB on Ser473, facilitating phosphorylation of Akt/PKB on Thr308 by PDK1 and required for the full activation of Akt/PKB (8).

Recently raptor has been identified as a direct substrate of the AMP-activated protein kinase (AMPK) (9). AMPK phosphorylates raptor on Ser722/Ser792 (9). This phosphorylation is essential for inhibition of the raptor-containing mTOR complex 1 (mTORC1) and induces cell cycle arrest when cells are stressed for energy (9). These findings suggest that raptor is a critical switch that correlates cell cycle progression with energy status.

1.  Hara, K. et al. (2002) Cell 110, 177-189.

2.  Sarbassov, D.D. et al. (2004) Curr. Biol. 14, 1296-1302.

3.  Beugnet, A. et al. (2003) J. Biol. Chem. 278, 40717-40722.

4.  Nojima, H. et al. (2003) J. Biol. Chem. 278, 15461-15464.

5.  Gwinn, D.M. et al. (2008) Mol Cell 30, 214-26.

6.  Oshiro, N. et al. (2004) Genes Cells 9, 359-366.

7.  Sarbassov, D.D. et al. (2005) Science 307, 1098-101.

8.  Kim, D. H. et al. (2003) Mol. Cell 11, 895-904.

9.  Kim, D. H. et al. (2002) Cell 110, 163-175.

Entrez-Gene Id 57521
Swiss-Prot Acc. Q8N122

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.

Raptor (24C12) Rabbit mAb (Sepharose® Bead Conjugate)